基于萝卜高密度遗传图谱的抽薹和开花性状的QTL定位

doi:10.16420/j.issn.0513-353x.2021-0201

摘要/Abstract

摘要：

对萝卜（Raphanus sativus L.）抽薹和开花性状的基因进行了定位研究,挖掘了调控抽薹开花的候选基因。主要结果如下：利用已构建的高密度遗传图谱,通过对F_2︰3家系的抽薹和开花性状的初步定位,检测到两个QTL位点与萝卜的抽薹开花相关。通过萝卜基因注释分析和亲本间的基因序列比对分析,检测出5个基因与萝卜抽薹和开花性状相关,其具体作用还需进一步验证。

关键词: 萝卜, 抽薹, 开花, QTL, 候选基因

Abstract:

The late-bolting genes in radish were localized,and the candidate genes regulating flowering were explored. The main results are listed below：a high-density genetic linkage map was constructed and two QTL loci related to flowering and bolting were detected. Based on radish gene annotation and gene sequence alignment analysis,we detected five genes with the sequence variation between the parental lines,indicated those genes associated with bolting and flowering in radish. However,the detailed functions of the candidate gene during the radish bolting and flowering need further studies.

Key words: Raphanus sativus, bolting, flowering, QTL, candidate gene

中图分类号:

S631.1

甘彩霞, 崔磊, 庞文星, 汪爱华, 於校青, 邓晓辉, 宋莉萍, 朴钟云. 基于萝卜高密度遗传图谱的抽薹和开花性状的QTL定位[J]. 园艺学报, 2021, 48(7): 1273-1281.

GAN Caixia, CUI Lei, PANG Wenxing, WANG Aihua, YU Xiaoqing, DENG Xiaohui, SONG Liping, PIAO Zhongyun. QTL Mapping of Bolting and Flowering Traits Based on High Density Genetic Map of Radish[J]. Acta Horticulturae Sinica, 2021, 48(7): 1273-1281.

图/表 7

参考文献 28

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播种年份/季节 Year/Season	世代 Generation	植株数量 Number of plants	抽薹时间/d Bolting time	开花时间/d Flowering time
2015/冬 2015/Winter	P₁	30	205	214
	P₂	30	138	155
	F₁	30	170	187
	F₂	129	180（150 ~ 193）	188（169 ~ 202）
2016/秋 2016/Autumn	P₁	30	207	220
	P₂	30	154	172
	F₁	30	167	184
	F_2︰3	97	171 ~ 195	185 ~ 207
2016/冬 2016/Winter	P₁	30	185	195
	P₂	30	140	147
	F₁	30	160	171
	F_2︰3	97	153 ~ 172	158 ~ 182

播种年份/季节 Year/Season	世代 Generation	植株数量 Number of plants	抽薹时间/d Bolting time	开花时间/d Flowering time
2015/冬 2015/Winter	P₁	30	205	214
	P₂	30	138	155
	F₁	30	170	187
	F₂	129	180（150 ~ 193）	188（169 ~ 202）
2016/秋 2016/Autumn	P₁	30	207	220
	P₂	30	154	172
	F₁	30	167	184
	F_2︰3	97	171 ~ 195	185 ~ 207
2016/冬 2016/Winter	P₁	30	185	195
	P₂	30	140	147
	F₁	30	160	171
	F_2︰3	97	153 ~ 172	158 ~ 182

连锁群 LG	QTL	季节 Season	置信区间/cM Confidence interval	标记区间 Marker interval	LOD		加性效应 Additive	显性效应 Dominance	R²/%
R01	qBT1	秋Autumn	3.61 ~ 11.51	R01_48351323 ~ R01_45976870	2.83		-0.20	-8.09	0.02
R09	qBT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	9.62		-12.04	11.67	0.60
R09	qBT2	冬Winter	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	13.37		-32.37	44.39	0.62
R01	qFT1	秋Autumn	6.67 ~ 8.52	R01_47574115 ~ R01_47530802	3.67	-0.07		-12.14	0.03
R09	qFT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	11.90	-18.05		12.08	0.86
		冬Winter			12.66	-38.79		41.38	0.59

连锁群 LG	QTL	季节 Season	置信区间/cM Confidence interval	标记区间 Marker interval	LOD		加性效应 Additive	显性效应 Dominance	R²/%
R01	qBT1	秋Autumn	3.61 ~ 11.51	R01_48351323 ~ R01_45976870	2.83		-0.20	-8.09	0.02
R09	qBT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	9.62		-12.04	11.67	0.60
R09	qBT2	冬Winter	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	13.37		-32.37	44.39	0.62
R01	qFT1	秋Autumn	6.67 ~ 8.52	R01_47574115 ~ R01_47530802	3.67	-0.07		-12.14	0.03
R09	qFT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	11.90	-18.05		12.08	0.86
		冬Winter			12.66	-38.79		41.38	0.59