园艺学报 ›› 2018, Vol. 45 ›› Issue (3): 409-420.doi: 10.16420/j.issn.0513-353x.2017-0686

• 研究论文 •    下一篇


孙天骅1,李 佳1,王 涛1,牛 宁2,徐继忠1,*   

  1. (1河北农业大学园艺学院,河北保定 071001;2石家庄市农林科学研究院,石家庄 050041)
  • 出版日期:2018-03-25 发布日期:2018-03-25

Difference in Protein Expression of Apple Leaves with Different Resistance Response to Botryosphaeria berengeriana f. sp. piricola Infection

SUN Tianhua1,LI Jia1,WANG Tao1,NIU Ning2,and XU Jizhong1,*   

  1. (1College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China;2 Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041,China)
  • Online:2018-03-25 Published:2018-03-25

摘要: 从‘鸡冠’与‘富士’苹果的杂交后代中选择对轮纹病抗病的材料1-1-24和感病材料1-2-34的叶片为试材,在叶片正面主脉两侧各针刺一处,分别接种长有轮纹病菌(Botryosphaeria berengeriana f. sp. piricola)菌丝和蘸有无菌蒸馏水的PDA培养基饼。通过对接菌前和接菌后24 h的叶片总蛋白进行2-DE分离筛选和质谱检测鉴定,共获得27个差异蛋白,其中9个为胁迫应答蛋白,1个与防御反应相关,17个参与叶绿体光合作用、细胞代谢、核糖体、羧酸等相关合成或未知反应。对β–1,3–葡聚糖酶、酸性内切几丁质酶和AP15基因进行实时定量PCR分析,结果表明:β–1,3–葡聚糖酶和酸性内切几丁质酶是苹果叶片应答轮纹病胁迫的关键蛋白,抗病和感病的苹果叶片在接菌前关键蛋白含量的显著差异可能是导致抗病性不同的原因之一。

关键词: 苹果, 轮纹病, 蛋白质组, 关键蛋白

Abstract: Two apple test materials 1-1-24 and 1-2-34 generated from hybrids of‘Jiguan’&‘Fuji’ were chosen as experimental materials. 1-1-24 was resistant to ring rot disease while 1-2-34 was highly susceptible. Each needle was stabbed on both sides of main vein of the blade. Culture medium cakes covered with Botryosphaeria berengeriana f. sp. piricola and sterile distilled water were separately inoculated to different blades. Total protein of apple leaves before treatment and after 24 h of treatment were suitable for the analysis of 2-DE and identified by mass spectrometry. There were 27 proteins successfully identified. During the process of apple leaves response to the ring rot pathogen stress,multiple pathways were pulsed on to achieve to stress related,defense related,cellular metabolism,synthesis related,photosynthesis and unknown. Combined with real-time quantitative PCR analysis of Glucanendo-1,3-beta-glucosidase,AP15 and acidic endochitinase,we came to the conclusion that Glucanendo-1,3-beta-glucosidase and acidic endochitinase played an important role in resistance to B.berengeriana f. sp. piricola and the key proteins’ extremely significant difference in content without pathogen infection probably was one of the reasons for the resistance difference.

Key words: apple, Botryosphaeria berengeriana f. sp. Piricola, proteomic, key protein