关键词: 越橘, 转色期果实, 紫外光, 花青苷, 酚类物质, 酶活性, 基因表达

Abstract:

This experiment was based on the changes between the content of anthocyanins and the activities of biosynthetic enzymes in blueberry during the development，which aimed at further testing accumulations of anthocyanins and other phenolic compounds in blueberry and the enzyme activities，transcriptional level of anthocyanin biosynthetic gene under the environmental stimulus of UV-A，UV-B，UV-C with the veraison fruit of 3-year old blueberry‘Northland’. The results indicated that three types of UV irradiation both significantly induced the accumulation of anthocyanins in blueberry，especially UV-C，increased by 236% after irradiation. In addition，the activities of PAL，UFGT and the expression of VcPAL，VcUFGT mRNA were induced by UV irradiation，but the enzyme activity，transcriptional level of VcDFR were suppressed. Among them，PAL，UFGT enzyme activities were significantly positively correlated with the accumulation of anthocyanin（r = 0.807^**，r = 0.894^**），while DFR was the opposite（r =–0.854^**）. The results suggest that UV irradiation can induced some key genes，which responded to UV treatment，transcriptional enhancement（such as VcPAL，VcUFGT）or inhibition（such as VcDFR），and affected the changes of the corresponding enzyme activities，which resulted in the accumulation of anthocyanin and other phenolic compounds.

Key words: blueberry, anthocyanin；UV, veraison fruit, phenolic compound, enzyme activity, gene expression