Cloning and Function Analysis of ELF4 Homolog Genes in Dimocarpus longan

doi:10.16420/j.issn.0513-353x.2017-0647

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (5): 875-886.doi: 10.16420/j.issn.0513-353x.2017-0647

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Cloning and Function Analysis of ELF4 Homolog Genes in Dimocarpus longan

FU Zhiyuan，JIA Tianqi，PENG Yuan，Saquib Weheed，and ZENG Lihui*

（College of Horticulture，Fujian Agriculture and Forestry University，Fuzhou 350002，China）

Online:2018-05-25 Published:2018-05-25

Abstract

Abstract:

The full length of cDNA sequences of two ELF4 homolog genes were cloned from Dimocarpus longan‘Red Seed’，named DlELF4-1 and DlELF4-2. The functions of these two genes were analyzed by bioinformatics analysis and real-time quantitative PCR. To further study the function of the DlELF4 genes，DlELF4-1 and DlELF4-2 were overexpressed in Arabidopsis thaliana. Sequence analysis and cluster analysis showed that DlELF4-2 was similar to AtELF4-L1，while DlELF4-1 and AtELF4 showed higher similarity. Overexpression of two DlELF4 genes separately in Arabidopsis thaliana resulted in delaying flowering and adventitious roots phenotypes，suggesting that DlELF4 genes may be repressors of flowering and promoting auxin synthesis. However，expression patterns of DlELF4-1 and DlELF4-2 genes were different during bud differentiation of longan，indicating that the two genes may have different functions in longan.

Key words: longan, ELF4, flowering bud differentiation, gene cloning, gene expression

CLC Number:

S 667.2

FU Zhiyuan，JIA Tianqi，PENG Yuan，Saquib Weheed，and ZENG Lihui*. Cloning and Function Analysis of ELF4 Homolog Genes in Dimocarpus longan[J]. ACTA HORTICULTURAE SINICA, 2018, 45(5): 875-886.

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Cloning and Function Analysis of ELF4 Homolog Genes in Dimocarpus longan

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