4种固化剂对若干草坪草愈伤组织诱导的影响

园艺学报 ›› 2006, Vol. 33 ›› Issue (2): 437-440.

4种固化剂对若干草坪草愈伤组织诱导的影响

王贺飞;柴明良^*

(浙江大学园艺系, 农业部园艺植物生长发育与生物技术重点开放实验室, 杭州310029)

收稿日期:2005-05-23 修回日期:2005-08-19 出版日期:2006-04-25 发布日期:2006-04-25

Effect of Four Brands of Gelling Agents on Callus Induction from Mature Seeds of Several Turfgrass Species

Wang Hefei;Chai Mingliang^*

(Department of Horticulture, Zhejiang University, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Hangzhou 310029, China)

Received:2005-05-23 Revised:2005-08-19 Online:2006-04-25 Published:2006-04-25

摘要/Abstract

摘要： 比较了Phytagel、Canageenen (卡拉胶) 、Agar A 和Agar powder对匍匐翦股颖(Agrostis palustris Huds. ) 品种‘L293’; 草地早熟禾(Poa pratensis L. ) 品种‘佛特纳’; 高羊茅( Festuca arundinacea Schreb) 品种‘新秀’、‘翠碧’; 结缕草( Zoysia japonica Steud. ) 品种‘青岛结缕草’、‘Zenith’和‘松南结缕草’及普通狗牙根(Cynodon dactylon L. ) 品种‘佳宝’成熟种子愈伤组织诱导和胚性愈伤组织发生的影响。结果表明, 就匍匐翦股颖、草地早熟禾而言, 无论是愈伤组织诱导率还是胚性愈伤组织发生率, 均以在Phytagel固化的培养基中最高。高羊茅在Agar powder上愈伤组织诱导率最高, 但就胚性愈伤发生而言固化剂之间无显著差异。虽然‘Zenith’和‘松南结缕草’在愈伤组织诱导上4种固化剂无显著差异, 但就胚性愈伤组织发生而言, ‘青岛结缕草’、‘Zenith’和‘松南结缕草’依次在Phytagel、Canageenen和Agar powder为佳。只有在Phytagel固化的培养基上普通狗牙根形成了胚性愈伤组织。

关键词: 草坪草, 固化剂, 愈伤组织, 胚性愈伤组织, 种子

Abstract: The effects of Phytagel, Canageenen, Agar A and Agar powder on callus induction and embryogenic callus development from the mature seeds of Agrostis palustris‘L293’, Poa pratensis ‘Fortuna’,Festuca arundinacea‘Starlet’and ‘Triple’, Zoysia japonica‘Qingdao’, ‘Zenith’and ‘Songnan’and Cynodon dactylon ‘Jackpot’were systematically compared. The results indicated that both the percentages of callus induction and the percentages of embryogenic callus development were the highest for Agrostis palustris and Poa pratensis on the medium solidified with Phytagel. Although the highest percentage of callus induction was obtained on the Agar powder solidified medium for Festuca arundinacea, no significant differences among
the four gelling agents were found on the development of emryogenic callus. Although the differences were not significant among the four gelling agents on callus induction from Zoysia japonica ‘Zenith’and ‘Songnan’,the development of embryogenic callus was highest on Phytagel, Canageenen and Agar power for‘Qingdao’,‘Zenith’and ‘Songnan’, respectively. For Cynodon dactylon, embryogenic callus was formed only on the medium solidified with Phytagel.

Key words: Turfgrass, Gelling agent, Callus, Embryogenic callus, Seed

王贺飞;柴明良. 4种固化剂对若干草坪草愈伤组织诱导的影响[J]. 园艺学报, 2006, 33(2): 437-440.

Wang Hefei;Chai Mingliang. Effect of Four Brands of Gelling Agents on Callus Induction from Mature Seeds of Several Turfgrass Species[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 437-440.

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