羊蹄甲属3种园艺树种分子鉴定及亲缘关系的ISSR分析

园艺学报 ›› 2006, Vol. 33 ›› Issue (2): 433-436.

羊蹄甲属3种园艺树种分子鉴定及亲缘关系的ISSR分析

罗瑜萍¹;龚　维¹;邱英雄^1*;Yuk Sing Gibert Chan^2*

(¹ 浙江大学生命科学学院, 杭州310029; ² 香港理工大学应用生物与化学系, 香港)

收稿日期:2005-06-01 修回日期:2005-08-09 出版日期:2006-04-25 发布日期:2006-04-25

Dentification of Three Species in Bauhinia and Hybrid Origin of Bauhinia blakeana Using ISSR Markers

Luo Yuping¹;GongWei¹;Qiu Yingxiong^1*;Yuk Sing Gibert Chan^2*

(¹Laboratory of Systematic and Evolutionary Botany and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou 310029, China; ²Department of Applied Biology and Chemical Technology, the Hongkong Polytechnic University, Hung Hom, Hongkong, China)

Received:2005-06-01 Revised:2005-08-09 Online:2006-04-25 Published:2006-04-25

摘要/Abstract

摘要： 利用ISSR-PCR方法对香港羊蹄甲属(Bauhinia) 中最常见3种园艺树种: 红花羊蹄甲(B. purpurea) 、宫粉羊蹄甲(B. variegate) 和洋紫荆(B. blakeana) 的遗传变异进行了研究, 从74个ISSR引物中筛选出9个多态性引物用于正式扩增, 共扩增出80条DNA带, 平均每个引物扩增的DNA带的数目为8.88条, 多态性条带数目为55条, 占总条带数的68.8%。其中, 有8个引物扩增出差异条带和特异条带,并分别能准确地鉴定羊蹄甲属3个园艺树种。本研究的结果表明, ISSR - PCR的DNA电泳谱带在检测香港羊蹄甲(B. blakeana) 品种的真实性方面非常有用。同时, 通过对3个种扩增条带的叠加性进行分析, 证实了洋紫荆杂交起源的父母本为红花羊蹄甲和宫粉羊蹄甲。

关键词: 羊蹄甲属, 洋紫荆, 红花羊蹄甲, 宫粉羊蹄甲, ISSR, 指纹图谱

Abstract: In this study, inter-simple sequence repeat (ISSR) was evaluated for its potential use in the identification of Bauhinia purpurea, B. blakeana and B. variegate. Of the 74 ISSR primers subjected to screening, 9 produced reproducible, polymorphic and discrepant fragments. Using these primers, 80 discernible DNA fragmentswere generated with 55 (68.8% ) being polymorphic bands. The average number of DNA bands amplified by each primer was 8.88. DNA profiles based on 8 primers have revealed potential diagnostic fingerprints which could contribute to the identification of 3 species in Bauhinia. Meanwhile, ISSR - PCR analysis revealed that the amplified fragments respectively unique to B. purpurea and B. variegate showed additivity in B. blakeana. The molecular evidence indicated that B. blakeana was a hybrid origin from B. purpurea and B. variegate.

Key words: Bauhinia, B. blakeana, B. purpurea, B. variegate, ISSR, DNA fingerprint

罗瑜萍;龚　维;邱英雄;Yuk Sing Gibert Chan. 羊蹄甲属3种园艺树种分子鉴定及亲缘关系的ISSR分析[J]. 园艺学报, 2006, 33(2): 433-436.

Luo Yuping;GongWei;Qiu Yingxiong;Yuk Sing Gibert Chan. Dentification of Three Species in Bauhinia and Hybrid Origin of Bauhinia blakeana Using ISSR Markers[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 433-436.

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