梨栽培品种SSR鉴定及遗传多样性

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 305-310.

梨栽培品种SSR鉴定及遗传多样性

曹玉芬¹;刘凤之¹;高　源¹; 姜立杰2;王　昆¹;马智勇¹; 张开春^2*

(¹中国农业科学院果树研究所, 辽宁兴城125100; ²北京市农林科学院林业果树研究所, 北京100093)

收稿日期:2006-07-11 修回日期:2006-12-04 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 张开春

SSR Analysis of Genetic Diversity of Pear Cultivars

CAO Yu-fen¹, L IU Feng-zhi¹, GAO Yuan¹, JIANG L i-jie², WANG Kun¹, MA Zhi-yong¹, and ZHANG Kai-chun^2*

(¹Research Institute of Pom ology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China; ²Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China)

Received:2006-07-11 Revised:2006-12-04 Online:2007-04-25 Published:2007-04-25
Contact: ZHANG Kai-chun

摘要/Abstract

摘要： 应用SSR技术对梨41个栽培品种进行遗传多样性分析, 12对SSR引物扩增出114个等位基
因, 平均每个位点915个。位点杂合度在0.1517～0.7079之间, 遗传多样性指数为0.4630。用两对引物(BGT23b和CHO2D11) 即可区分除芽变品种之外的全部供试品种。系统聚类分析将41个梨品种分为2大类, 即西洋梨和东方梨。原产中国的秋子梨、白梨和部分砂梨品种归类相互交错; 库尔勒香梨和其他新疆梨聚在一起; 我国砂梨品种宝珠梨、德胜香, 日本砂梨品种新世纪、丰水以及韩国砂梨品种黄金梨聚在一起。秋白与蜜梨, 南果梨与满园香、秋子、八里香相似系数高, 分别属于同一品种群。金花梨与金川雪、苍溪雪起源演化相关。

关键词: 梨, 栽培品种, 遗传多样性, SSR

Abstract: Genetic diversity of 41 pear cultivars was analyzed by using SSR markers. Twelve SSR markers amp lified 114 alleles, with an average of 915 alleles per locus. Locus heterozygosity was 0.1517-0.7079, and the genetic diversity index was 014630. Two pairs of p rimer (BGT23b and CHO2D11) could
distinguish all of the pear cultivars except the mutant cultivars. UPGMA cluster analysis showed that the cultivars could be classified into two groups, the occidental pear and oriental pear. Pyrus bretschneideri Rehd. , P. ussuriensis Maxim. , and some P. pyrifolia (Burm. f. ) Nakai cultivars originated in China
interveined each other; Kuerlexiang and other P. sinkiangensis Yüvarieties could be clustered together. Chinese P. py rifolia Baozhu and Deshengxiang, Japanese P. pyrifolia Shinseiki and Housui, Korean P. py rifolia Whangkeumbae were clustered together in the dendrogram. The coefficient between Qiubai andMiliwas high,and they belonged to the same population, so as Nanguo, Manyuanxiang, Qiuzi and Balixiang. Jinhua originated correlatively with J inchuanxue and Cangxixue.

Key words: Pear, Cultivar, Genetic diversity, SSR

中图分类号:

S 661.2

曹玉芬;刘凤之;高　源;姜立杰;王　昆;马智勇;张开春. 梨栽培品种SSR鉴定及遗传多样性[J]. 园艺学报, 2007, 34(2): 305-310.

CAO Yu-fen;L IU Feng-zhi;GAO Yuan;JIANG L i-jie;WANG Kun;MA Zhi-yong;and ZHANG Kai-chun. SSR Analysis of Genetic Diversity of Pear Cultivars[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 305-310.

[1]	王瑞, 洪文娟, 罗华, 赵丽娜, 陈颖, 王君, . 石榴品种SSR指纹图谱构建及杂种父本鉴定[J]. 园艺学报, 2023, 50(2): 265-278.
[2]	王梦梦, 孙德岭, 陈锐, 杨迎霞, 张冠, 吕明杰, 王倩, 谢添羽, 牛国保, 单晓政, 谭津, 姚星伟, . 花椰菜核心种质的构建与评价[J]. 园艺学报, 2023, 50(2): 421-431.
[3]	刘艺平, 倪梦辉, 吴芳芳, 刘红利, 贺丹, 孔德政. 荷花花器官性状与SSR标记的关联分析[J]. 园艺学报, 2023, 50(1): 103-115.
[4]	宋健坤, 杨英杰, 李鼎立, 马春晖, 王彩虹, 王然. 梨新品种‘鲁秀’[J]. 园艺学报, 2022, 49(S2): 3-4.
[5]	董星光, 曹玉芬, 张莹, 田路明, 霍宏亮, 齐丹, 徐家玉, 刘超, 王立东. 抗寒脆肉梨新品种‘玉翠香’[J]. 园艺学报, 2022, 49(S2): 5-6.
[6]	欧春青, 姜淑苓, 王斐, 马力, 张艳杰, 刘振杰. 早熟梨新品种‘兴梨蜜水’[J]. 园艺学报, 2022, 49(S2): 7-8.
[7]	张艳杰, 王斐, 欧春青, 马力, 姜淑苓, 刘振杰. 梨新品种‘中梨玉脆3’[J]. 园艺学报, 2022, 49(S2): 9-10.
[8]	范净, 陈启亮, 张靖国, 杨晓平, 杜威, 田瑞, 周德平, 胡红菊, . 中熟红皮砂梨新品种‘金彤’[J]. 园艺学报, 2022, 49(S2): 11-12.
[9]	王苏珂, 李秀根, 杨健, 王龙, 苏艳丽, 张向展, 薛华柏. 红皮梨新品种‘丹霞红’[J]. 园艺学报, 2022, 49(S2): 13-14.
[10]	王斐, 欧春青, 张艳杰, 马力, 姜淑苓. 晚熟耐贮梨新品种‘华秋’[J]. 园艺学报, 2022, 49(S1): 9-10.
[11]	宋健坤, 李鼎立, 杨英杰, 马春晖, 王彩虹, 王然. 梨新品种‘琴岛红’[J]. 园艺学报, 2022, 49(S1): 11-12.
[12]	郭伟珍, 赵京献, 李莹. 中早熟梨新品种‘美玉’[J]. 园艺学报, 2022, 49(9): 2051-2052.
[13]	刘金明, 郭彩华, 袁星, 亢超, 全绍文, 牛建新. 梨Dof家族基因鉴定及其在宿存与脱落萼片中的表达分析[J]. 园艺学报, 2022, 49(8): 1637-1649.
[14]	陶鑫, 朱荣香, 贡鑫, 吴磊, 张绍铃, 赵建荣, 张虎平. 梨果糖激酶基因PpyFRK5在果实蔗糖积累中的作用[J]. 园艺学报, 2022, 49(7): 1429-1440.
[15]	张秋悦, 刘昌来, 于晓晶, 杨甲定, 封超年. 盐胁迫条件下杜梨叶片差异表达基因qRT-PCR内参基因筛选[J]. 园艺学报, 2022, 49(7): 1557-1570.