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园艺学报 ›› 2019, Vol. 46 ›› Issue (10): 1882-1896.doi: 10.16420/j.issn.0513-353x.2018-0842

• 研究论文 • 上一篇    下一篇

葡萄MADS-box转录因子家族全基因组鉴定及表达分析

黄晓婧1,张 珺1,夏 惠1,2,邓群仙1,王 进1,2,吕秀兰1,2,梁 东1,2,*   

  1. 1四川农业大学园艺学院,成都 611130;2四川农业大学果蔬研究所,成都 611130
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    四川省科技厅应用基础项目(2017JY0169);四川省科技基础条件平台项目(18PTDJ0140,2019JDPT0009)

Genome-wide Identification and Expression Analysis of the MADS-box Gene Family in Vitis vinifera

HUANG Xiaojing1,ZHANG Jun1,XIA Hui1,2,DENG Qunxian1,WANG Jin1,2,Lü Xiulan1,2,and LIANG Dong1,2,*   

  1. 1College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;2Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China
  • Online:2019-10-25 Published:2019-10-25

摘要: 从葡萄基因组中共鉴定出54个MADS-box基因,其中Type-Ⅰ型10个,Type-Ⅱ型44个,分布于15条染色体中,编码的蛋白质序列为62 ~ 596个氨基酸,外显子数1 ~ 16个。启动子区含有大量光信号、植物激素、胁迫和分生组织等相关功能域。根据实时荧光定量RT-PCR的结果发现,SEP、FUL、FLC、SVP、SOC1和ANR1亚类基因随叶片生长表达水平逐步升高,在成熟叶中最高,衰老叶片中下降;果实第1次膨大期和果实转色期是SEP、SOC1和ANR1亚类基因的两个表达高峰期;单氰胺处理诱导SVP、AGL15、SOC1、AP3/PI、AGL12和FLC亚类基因在休眠过程中表达量持续上升,在休眠解除过程表达量下降。

关键词: 葡萄, MADS-box家族, 基因表达, 休眠, 单氰胺

Abstract: A total of 54 MADS-box genes were identified from the grape genome,including 10 Type-Ⅰand 44 Type-Ⅱgenes. All of them distributed on 15 chromosomes,encoded protein sequence was 62–596 amino acids,and the number of exons 1–16 pieces. The promoter region contains a large number of related functional domains such as light signal,plant hormone,stress and meristem. According to the results of qRT-PCR,the expression level of SEP,FUL,FLC,SVP,SOC1 and ANR1 gradually increased with the leaves growth,reaching the highest in mature leaves,then decreased in old leaves;the first fruit expansion period and fruit color change period are the two peak expression stages of SEP,SOC1 and ANR1 subclass genes;hydrogen cyanamide treatment induced the expression of SVP,AGL15,SOC1,AP3/PI,AGL12 and FLC subclass genes to increase continuously during dormancy and decrease during dormancy release.

Key words: grape, MADS-box gene family, gene expression, dormancy, hydrogen cyanamide

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