关键词: 葡萄, NAC转录因子, 矮化, 卷叶

Abstract: In this study，a NAC gene，VvDRL1，was isolated from Vitis vinifera‘Yatomi Rosa’. The length of full VvDRL1 was 840 bp，encoding 280 amino acids，which shared a 100% similarity in cRNA and 6 single nucleotide mutation in DNA with‘Pinot Noir’. Subcellular localization showed that VvDRL1 is a nuclear protein. Transgenic tobacco with VvDRL1 was obtained by Agrobacterium-mediated method. Compared with the wild type，the growth of T2-generation transgenic tobacco presented delayed with the plant height，leaf area and mesophyll cells area of about 60%，34% and 31% in comparison with wild tobacco plants. In addition，the transgenic tobacco showed curly leaves. Through paraffin sections observation，it is found that the upper epidermis in main leaf veins of transgenic tobacco lacked 2–3 layers sclerenchyma cells and had significantly more space in the spongy tissue compared with the wild type. Based on the above study，it is inferred that VvDRL1 gene play an important role in the regulation of plant morphological development.

Key words: Vitis vinifera, NAC transcription factor, dwarf, curly leaf