关键词: 柑橘, CitERF9, CitAP2-7, 基因表达, 激素, 逆境胁迫

Abstract:

Two AP2/ERF genes，CitERF9 and CitAP2-7 were analyzed in Citrus junos‘Ziyang’. The
data showed that CitERF9 genomic sequence contained a single exon of 735 bp long，which could encode
a protein of 244 amino acid residues. In contrast，CitAP2-7 genomic sequence possessed 5 introns and its 6
exons could encode a protein of 360 amino acid residues. Sequence alignment showed that CitERF9 and
CitAP2-7 shared 81%–84% amino acid identities with corresponding AP2/ERF members from soybean，
castor，peach and alfalfa. Real time quantitative analysis revealed that the expression of CitAP2-7 and
CitERF9 in different tissues exhibited different patterns. In roots，CitERF9 was induced by all hormone
and stress treatments，whereas CitAP2-7 was mainly modulated by NaCl，ABA and dehydration. In leaves，
CitERF9 was up-regulated by all treatments，similar to that in roots. But CitAP2-7 was suppressed by
ABA，ACC，MeJA，SA and NaCl treatments. The results suggested the involvement of both CitERF9 andCitAP2-7 in the responses of citrus to stresses and relevant hormones.

Key words: citrus, CitERF9, CitAP2-7, gene expression, hormone, stress