关键词: 青花菜, 耐贮藏, 叶绿素降解基因, 基因表达

Abstract: Two different storage properties broccoli‘8554’and‘90196’were used for the study. In this work，the changes of chlorophyll content and the relative expression levels of chlorophyll degrading genes in the two high-generation inbred lines were analyzed. The results showed that the chlorophyll contents of the two materials showed decreased during the storage，while‘8554’declined slowly and remained higher compared with‘90196’. The expression of BoCLH1（chlorophyllase 1）showed the samebehavior in the two materials，it was detected a higher expression before storage，then decreased to a low level during the storage. The expression of BoCLH2（chlorophyllase 2）changed slightly in‘8554’，however it changed largely in‘90196’，and the expression level was higher than‘8554’.The expression of BoPPH（pheophytinase）declined in‘8554’during the whole storage，but it had a evident increase at the 14th day in‘90196’. The expression of BoPaO（pheide a oxygenase）had a sharp increase in both of them，but the day appeared in‘8554’was later than‘90196’. The obvious expression peak of BoRCCR（red chlorophyll catabolite reductase）appeared in‘90196’，but it did not appear in‘8554’. Those results indicated that the mechanism of chlorophyll degradation was different in different storage properties broccoli，the changes of chlorophyll content were mainly regulated by the expression levels of BoCLH2，BoPPH，BoPaO and BoRCCR in the senescence，and BoCLH1 was responsible for the degradation of chlorophyll in the initial stage.

Key words: broccoli, storage, chlorophyll degrading gene, gene expression