关键词: 梨, 地方品种, SSR, cpDNA, 遗传多样性, 亲缘关系

Abstract: A non-coding chloroplast region，accD-psaI and 17 microsatellite markers were used to assay genetic diversity of 49 pear accessions from Fujian Province. A total of 49 sequences were collected and five haplotypes（Hap1–Hap5）were recognized containing four variation sites. Nucleotide diversity（Pi）and haplotype diversity were 0.00139 and 0.660. The TCS Network indicated that Hap5 was the ancestral haplotype，only detected in two individuals. Two hundred and eleven different alleles were detected among seventeen SSR loci，ranged from 5 to 23 with an average of 12.41 alleles per locus. The mean observed and expected heterozygosity values were 0.5802 and 0.7549，respectively. The Shannon’s information index for each locus was from 0.5830 to 2.7683 with a mean value of 1.8661. The Neighbor-Joining（NJ）tree grouped 49 pear accessions to nine major groups，which are generally congruent to haplotype diversity. Chloroplast haplotypes and microsatellite locus polymorphism information demonstrated a high level of genetic diversity of pear landraces from Fujian，China.

Key words: pear, landraces, SSR, cpDNA, genetic diversity, genetic relationship