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园艺学报 ›› 2011, Vol. 38 ›› Issue (6): 1045-1051.

• 果树 • 上一篇    下一篇

苹果砧木枝条电阻抗参数与其抗寒性的关系

金明丽,徐继忠*,张 钢   

  1. (河北农业大学园艺学院,河北保定 071001)   
  • 收稿日期:2011-04-08 修回日期:2011-06-01 出版日期:2011-06-25 发布日期:2011-06-25
  • 通讯作者: 徐继忠

Relation Between Electrical Impedance Spectroscopy Parameters and Frost Hardiness in Shoots of Apple Rootstocks

JIN Ming-li,XU Ji-zhong*,and ZHANG Gang   

  1. (College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China)   
  • Received:2011-04-08 Revised:2011-06-01 Online:2011-06-25 Published:2011-06-25
  • Contact: XU Ji-zhong

摘要: 以18株2年生苹果砧木西府海棠(Malus micromalus Makino)实生后代单株为试材,研究了不经冷冻处理的枝条的电阻抗(EIS)参数与其抗寒性(电导法测定枝条冷冻半致死温度)的关系。结果表明,3个时期(3月、7月、11月)枝条的EIS参数高频电阻率r和低频电阻率r1、胞外电阻率re、弛豫时间τ与抗寒性呈显著负相关(r =﹣0.737** ~﹣0.877**),在11月时胞内电阻率ri与抗寒性也呈显著负相关(r =﹣0.857**),表明在非冷冻条件下可采用枝条的电阻率r和r1、胞外电阻率re、弛豫时间τ估测苹果砧木实生后代枝条的抗寒性。通过比较3个时期各参数的最终通径系数,得出re为估测枝条抗寒性最佳的参数。   

关键词: 苹果, 砧木, 实生后代, 电阻抗图谱, 抗寒性, 电阻, 弛豫时间

Abstract: The 18 two-year-old seedlings of the apple rootstocks(Malus micromalus Makino)were used to analyse the relation between non-frost-exposed electrical impedance spectroscopy(EIS)parameters and frost hardiness(LT50 measured by the electrolyte leakage)in shoots of apple rootstocks. Results showed that the high-frequency resistances(r)and low-frequency resistances(r1),extracellular resistance(re),as well as relaxation time(τ)significantly correlated with the frost hardiness(r from﹣0.737** to﹣0.877**)in three different periods(March,July and November),and intracellular resistance(r i)had significant correlation with the frost hardiness(r =﹣0.857**)in November. The results suggested that these EIS parameters could be used to evaluate the frost hardiness of apple seedling rootstocks shoots without exposure to artificial freezing and according to path coefficient of every parameter in the three periods,re was the best parameter.  

Key words: apple, rootstock, seedling plant, electrical impedance spectroscopy(EIS), frost hardiness, resistance, relaxation time

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