关键词: 葡萄, 过氧化氢, VvIPK2, 低温

Abstract: An inositol polyphosphate kinase gene named VvIPK2 from Vitis vinifera was cloned and its biomatics was analyzed，the role of H2O2 and the interaction between H2O2 and VvIPK2 in response to cold stress of‘F-242’leaves were investigated by real-time PCR，combined with physiological and pharmacological methods. The results showed that the deduced translation product（VvIPK2）was completely the same with the sequence in database which shares 64% and 62% identity with the inositol polyphosphate kinase（LuIPK2）in Linum usitatissimum and that in Arabidopsis（AtIPK2）. Low temperature induced H2O2 accumulation and enhanced VvIPK2 transcription level. Exogenous H2O2 could enhance expression of VvIPK2 and H2O2 cleaner（ascorbic acid，AsA）could reduce its expression. At the same time，exogenous H2O2 could enhance the activities of superoxide dismutase（SOD）and Cu/Zn SOD expression，reduce the content of malondialdehyde（MDA）and the relative permeability of cell membrane under cold stress. But LY-294002 had no effect on cold-induced H2O2 production. Moreover，LY-294002 and AsA reversed the changes of SOD，MDA and the relative permeability of cell membrane，implying that VvIPK2 was assumed to be the downstream of H2O2 in grape response to low temperature stress. 　

Key words: Vitis vinifera, hydrogen peroxide, VvIPK2, low temperature stress