园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 1-8.

• 果树 •    下一篇


姚玉新1;李明1; 由春香1 ;刘志2;王冬梅2; 郝玉金1*   

  1. (1 山东农业大学园艺科学与工程学院, 国家苹果工程技术研究中心, 作物生物学国家重点实验室, 山东泰安271018;2 辽宁省果树科学研究所, 辽宁熊岳115009)
  • 收稿日期:2009-07-28 修回日期:2009-11-04 出版日期:2010-01-25 发布日期:2010-01-25
  • 通讯作者: 郝玉金

Relationship Between Malic Acid Metabolism-related Key Enzymes and Accumulation of Malic Acid as Well as the Soluble Sugars in Apple Fruit

YAO Yu-xin1; LI Ming1; YOU Chun-xiang1;LIU Zhi2 ;WANG Dong-mei2;HAO Yu-jin1*   

  1. (1College of Horticultural Science and Engineering, Shandong Agricultural University, National Research Center for Apple Engineering and Technology, State Key Laboratory of Crop Biology, Tai'an, Shandong 271018, China; 2Liaoning Institute of Fruit Tree Science, Xiongyue, Liaoning 115009, China)
  • Received:2009-07-28 Revised:2009-11-04 Online:2010-01-25 Published:2010-01-25
  • Contact: HAO Yu-jin

摘要: 以‘东光’ ב富士’苹果杂交后代为试材, 研究苹果果实中苹果酸代谢关键酶与苹果酸和可溶性糖积累的关系。结果表明, 负责苹果酸合成的磷酸烯醇式丙酮酸羧化酶( PEPC) 、细胞质NAD依赖的苹果酸脱氢酶( cyMDH) 和负责苹果酸跨膜运输的H+-焦磷酸酶(VHP) 在高、低酸苹果果实成熟过程中活性没有明显差异, 而负责苹果酸跨膜运输的H + -ATPase酶(VHA) 在高酸果实中有较高的活性, 负责苹果酸降解的细胞质NADP依赖的苹果酸酶( cyME) 和ATP依赖的磷酸烯醇式丙酮酸羧化激酶( PEP-CK) 则在低酸果实中有较高的活性。还从苹果酸向可溶性糖转化的角度探讨了苹果酸代谢关键酶对可溶性糖积累的影响。

关键词: 苹果, 苹果酸代谢关键酶, 糖, 酸积累

Abstract: The relationship between activity of malic acid metabolism-related key enzymes and accumulation of malic acid aswell as the soluble sugars was investigated by the hybrid from the crossing between apple cultivar‘Dongguang’and ‘Fuji’. The results showed that cytosolic phosphoenolpyruvate carboxylase ( PEPC) and NAD-dependentmalate dehydrogenase (cyMDH) , implicated in malic acid (MA) synthesis in the cytosol, and vacuolar H+-pyrophosphatase (VHP) , implicated in MA storage in the vacuole, exhibited no significant difference between the high- and low-acid apple fruits. Whereas, vacuolar H+-ATPase (VHA) , involved in MA storage in the vacuole, had much higher enzyme activity in the high-acid fruit, while MA degradation-related cytosolic NADP-dependent malic enzyme ( cyME) and ATP-dependent phosphoenolpyruvate carboxylase kinase ( PEPCK) exhibited high enzyme activities in the low-acid fruit. In addition, the effects of these key enzymes on the accumulation of soluble sugarswere discussed in terms of the transformation of malic acid into sugars.

Key words: apple, key enzymes involved in malic acid metabolism, accumulations of malic acid and the soluble sugars