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园艺学报 ›› 2020, Vol. 47 ›› Issue (7): 1277-1288.doi: 10.16420/j.issn.0513-353x.2019-0995

• 研究论文 • 上一篇    下一篇

葡萄LIM家族基因特征及表达模式分析

毛 娟,卢世雄,王 萍,马宗桓,刘 涛,何红红,乃国洁,张志强,陈佰鸿*   

  1. 甘肃农业大学园艺学院,兰州 730070
  • 出版日期:2020-07-25 发布日期:2020-07-25
  • 基金资助:
    甘肃农业大学青年导师基金项目(GAU-QDFC-2019-05);甘肃省陇原创新团队项目(LQ20180918-1);甘肃省葡萄酒产业发展专项;甘肃省科技重大专项(18ZD2NA006)

Analysis of Gene Characteristic and Expression Pattern of LIM Family in Grape

MAO Juan,LU Shixiong,WANG Ping,MA Zonghuan,LIU Tao,HE Honghong,NAI Guojie,ZHANG Zhiqiang,and CHEN Baihong*   

  1. College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China
  • Online:2020-07-25 Published:2020-07-25

摘要: 为了明确LIM家族基因在葡萄非生物胁迫下的表达特性,在生物信息分析的基础上,以‘黑比诺’葡萄(Vitis vinifera‘Pinot Noir’)试管苗为试验材料,利用qRT-PCR技术检测了不同逆境处理对葡萄LIM家族基因成员表达的影响。结果显示,葡萄LIM基因家族有14个成员,可分为4个亚族,各亚族编码蛋白氨基酸数量差异较大,但亚族内差异较小;分析葡萄基因和拟南芥基因间的选择压发现,二者存在钝化选择关系;多序列比对发现,该家族基因成员含有1或2个锌指结构的保守结构域;保守基序分析结果表明,所有基因均包含motif2;基因结构显示VvLIM含有4 ~ 15个外显子;14个基因中有11个编码蛋白存在互作关系。该家族基因存在大量的光诱导元件和水杨酸等逆境胁迫相关元件。基因芯片表达数据表明,高表达的基因主要存在生长旺盛的组织中。qRT-PCR分析发现,VvLIM1在100 mmol ? L-1 NaCl、10% PEG和50 mg ? L-1 SA胁迫下表达量与对照相比显著上调,且随处理时间的延长表达量显著增加。

关键词: 葡萄, LIM基因家族, 生物信息学分析, qRT-PCR, 非生物胁迫

Abstract: In order to clarify the expression characteristics of LIM gene family in grape,the bioinformatics analysis was carried out,and then the gene expression levels of LIM family under different stress were detected by qRT-PCR using Vitis vinifera‘Pinot Noir’plantlets as experimental material. The results showed that the LIM gene family of grape had 14 members and could be divided into four subgroups. Through the analysis of the selective pressure between grape genes and Arabidopsis genes,there was a passivation selection relationship between the two. Multiple sequence alignments revealed that the members of this family contained 1–2 conserved domains of zinc finger structure. The conserved motif results showed that all genes contained motif2. The analysis of gene structure showed that VvLIM contains 4–15 exons. Of the 14 genes,11 interacted with encoded protein. There were a large number of photoinduced elements and stress related elements such as salicylic acid in this gene family. The microarray data showed that the highly expressed genes mainly existed in the growing tissues. Compared with the control,the expression levels of VvLIM1 under 100 mmol ? L-1NaCl,10% PEG,and 50 mg ? L-1 SA stresses were significantly up-regulated by qRT-PCR analysis,and kept increasing with the treatment time.

Key words: grape, gene family of LIM, bioinformatics analysis, qRT-PCR, abiotic stress

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