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园艺学报 ›› 2017, Vol. 44 ›› Issue (6): 1073-1084.doi: 10.16420/j.issn.0513-353x.2016-0693

• 研究论文 • 上一篇    下一篇

毛欧杂种葡萄‘NW196’花色苷组成与相关基因表达分析

成 果1,周思泓2,张 劲1,张 瑛1,管敬喜1,杨 莹1,谢林君1,文仁德1,*   

  1. (1广西农业科学院葡萄与葡萄酒研究所,南宁 530007;2广西作物遗传改良生物技术重点开放实验室,南宁 530007)
  • 出版日期:2017-06-25 发布日期:2017-06-25

Analysis of Anthocyanin Composition and Genes Expression Patterns of Vitis quinquangularis × V. vinifera‘NW196’

CHENG Guo1,ZHOU Sihong2,ZHANG Jin1,ZHANG Ying1,GUAN Jingxi1,YANG Ying1,XIE Linjun1,and WEN Rende1,*   

  1. (1Grape and Wine Reaserch Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;2Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Nanning 530007,China)
  • Online:2017-06-25 Published:2017-06-25

摘要:

以不同发育期毛欧杂种葡萄‘NW196’为试验材料,利用HPLC–MS检测其果皮中花色苷的组成及含量,Real-time PCR检测花色苷合成相关基因表达水平,并与欧亚种‘赤霞珠’比较。结果表明,成熟后期‘NW196’果皮中花色苷总量显著高于‘赤霞珠’;采收期‘NW196’葡萄果皮中共检测到23种花色苷,成熟后期单糖苷所占比例高于双糖苷,其中含量最高的是花翠素–3–O–葡萄糖苷,3’5’–羟基取代花色苷比例高于3’–羟基取代花色苷;与欧亚种‘赤霞珠’相比,‘NW196’果皮中酰化和甲基化花色苷比例较低。结论:毛欧杂种‘NW196’花色苷组成与欧亚种‘赤霞珠’差异明显,这与其品种特性有关,并受到类黄酮代谢路径相关基因的调控;此外,花色素双糖苷主要在转色初期合成。

关键词: 葡萄, 果皮, 花色苷, 基因表达, HPLC-MS

Abstract:

The composition and content of anthocyanins were surveyed by HPLC–MS in berry skin of‘NW196’(Vitis quinquangularis × V. vinifera),transcript levels of both structural and regulatory genes involved in anthocyanin synthesis confirmed by Real-time PCR,which were compared with V. vinifera L.‘Cabernet Sauvignon’. The results indicated that the‘NW196’berry skin had significantly higher total anthocyanin content than‘Cabernet Sauvignon’berry skin during late-ripening. At harvest,23 anthocyanins were detected in‘NW196’berry skins,and the berry skins of ‘NW196’contained higher proportions of monoglucoside anthocyanins than diglucoside anthocyanins during late-ripening. Delphinidin- 3-O-diglucoside was the most abundant anthocyanins in berry skin of‘NW196’,and 3’5’-substituted showed higher proportion than 3’-substituted anthocyanins. When compared with ‘Cabernet Sauvignon’,lower proportions of acylated and methylated anthocyanins detected in‘NW196’berry skins. These results suggested that the composition and content of anthocyanins in berry skins of‘NW196’and‘Cabernet Sauvignon’showed great differences,which related to the variety’s own characteristics and regulated by genes in flavonoid metabolic pathways. In addition,diglucoside anthocyanins were synthesized at the beginning of veraison.

Key words: grape, berry skins, anthocyanin, gene expression, HPLC–MS

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