Construction of Molecular Linkage Map in Grape Using RAPD Markers and F1 Population of Interspecific Cross

ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (1): 68-70.

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Construction of Molecular Linkage Map in Grape Using RAPD Markers and F₁ Population of Interspecific Cross

Luo Sulan^1,2, He Puchao², Zhou Peng³, and Zheng Xueqin³

(¹Department of Agronomy , Hainan University , Haikou 570228; ²Department of Horticulture , Northwestern Agricultural University , Yangling 712100;³ The National Key Biotechnology Laboratory for Tropical Crops , Haikou 571101)

Received:1900-01-01 Revised:1900-01-01 Online:2001-02-25 Published:2001-02-25

Abstract

Abstract: A set of 280 randomoligonucleotide perimers was screened for random amplified polymorphic DNAs (RAPD) fragments within a sample of 60 F₁hybrids to generate RAPD markers and construct the molecular linkage map fromtwo parents 8324296 (V. quinquangularis) and Muscat Rose (V. vinifera) . 89 repeatable RAPD markers as well as four morphological markers (flower type , grape downy mildew2resistance , berry color and juice color) were obtained. The map consisted of 11 linkage groups , it covered a total genetic distance of over 1 033 cM (centimorgan) , the average distance between markers was 17.8 cM. It supplied a linkage framework for a more saturated linkage map of grape.

Key words: RAPD, Molecular marker, Linkage map, Grape

CLC Number:

Luo Sulan;He Puchao;Zhou Peng;and Zheng Xueqin. Construction of Molecular Linkage Map in Grape Using RAPD Markers and F₁ Population of Interspecific Cross[J]. ACTA HORTICULTURAE SINICA, 2001, 28(1): 68-70.

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Construction of Molecular Linkage Map in Grape Using RAPD Markers and F₁ Population of Interspecific Cross

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