Establishment of Efficient Shoot Regeneration System of Chinese Cabbage (Brassica campestris ssp. pekinensis) Inbred Line‘AB-81’and Studies of Transient Expression of gus A Gene

ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (1): 74-76.

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Establishment of Efficient Shoot Regeneration System of Chinese Cabbage (Brassica campestris ssp. pekinensis) Inbred Line‘AB-81’and Studies of Transient Expression of gus A Gene

Wang Huoxu¹, Wang Guanlin¹, Wang Xiaoyan², Fang Hongjun¹, Jia Shirong³, Tang Yixiong³, and Wei Yutang⁴

(¹ Bioengineering Institute of Liaoning Normal University, Dalian 116029;²Dalian seed company, Dalian 116033;³Biotechnology center of the Chinese Agriculture Academy of Science, Beijing 100081;⁴ Department of Horticulture, Shenyang Agricultural University, Shenyang 110161)

Received:1900-01-01 Revised:1900-01-01 Online:2001-02-25 Published:2001-02-25

Abstract

Abstract: An efficient shoot regeneration systemwas established using cotyledon and leave cuts of the inbred line‘AB-81’as explants. On the MS medium supplemented with 10 mg/L AgNO₃ , 0. 2 mg/L TDZ, 2 mg/L NAA and 0.25mg/L ABA , the regeneration frequency of the cotyledon and leave explants was up to 93. 3% and 90% respectively. The mean number and the most number of shoots per explant was 3 - 6 and 21 respectively. Cotyledons of AB281 were inoculated with Agrobacterium tumefaciens strain EHA105 containing pMOG410 carrying gus A-intron gene. The optimum conditions for the transient expression of gus A gene were as follows : preculture time of 2 - 3 days , bacterium density of OD 0. 3 - 0.5 , infection time of 2 - 10 min , and coculture time of 2 - 3 days.

Key words: Chinese cabbage 〔Brassica campestris L. ssp. pekinensis (Lour) Olsson〕, Cotyledons, Leaves, Regeneration, Gus A gene

CLC Number:

Q 786
S 634

Wang Huoxu;Wang Guanlin;Wang Xiaoyan;Fang Hongjun;Jia Shirong;Tang Yixiong;and Wei Yutang. Establishment of Efficient Shoot Regeneration System of Chinese Cabbage (Brassica campestris ssp. pekinensis) Inbred Line‘AB-81’and Studies of Transient Expression of gus A Gene[J]. ACTA HORTICULTURAE SINICA, 2001, 28(1): 74-76.

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Establishment of Efficient Shoot Regeneration System of Chinese Cabbage (Brassica campestris ssp. pekinensis) Inbred Line‘AB-81’and Studies of Transient Expression of gus A Gene

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