Effect of Four Brands of Gelling Agents on Callus Induction from Mature Seeds of Several Turfgrass Species

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (2): 437-440.

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Effect of Four Brands of Gelling Agents on Callus Induction from Mature Seeds of Several Turfgrass Species

Wang Hefei;Chai Mingliang^*

(Department of Horticulture, Zhejiang University, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Hangzhou 310029, China)

Received:2005-05-23 Revised:2005-08-19 Online:2006-04-25 Published:2006-04-25

Abstract

Abstract: The effects of Phytagel, Canageenen, Agar A and Agar powder on callus induction and embryogenic callus development from the mature seeds of Agrostis palustris‘L293’, Poa pratensis ‘Fortuna’,Festuca arundinacea‘Starlet’and ‘Triple’, Zoysia japonica‘Qingdao’, ‘Zenith’and ‘Songnan’and Cynodon dactylon ‘Jackpot’were systematically compared. The results indicated that both the percentages of callus induction and the percentages of embryogenic callus development were the highest for Agrostis palustris and Poa pratensis on the medium solidified with Phytagel. Although the highest percentage of callus induction was obtained on the Agar powder solidified medium for Festuca arundinacea, no significant differences among
the four gelling agents were found on the development of emryogenic callus. Although the differences were not significant among the four gelling agents on callus induction from Zoysia japonica ‘Zenith’and ‘Songnan’,the development of embryogenic callus was highest on Phytagel, Canageenen and Agar power for‘Qingdao’,‘Zenith’and ‘Songnan’, respectively. For Cynodon dactylon, embryogenic callus was formed only on the medium solidified with Phytagel.

Key words: Turfgrass, Gelling agent, Callus, Embryogenic callus, Seed

Wang Hefei;Chai Mingliang. Effect of Four Brands of Gelling Agents on Callus Induction from Mature Seeds of Several Turfgrass Species[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 437-440.

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Effect of Four Brands of Gelling Agents on Callus Induction from Mature Seeds of Several Turfgrass Species

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