ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (10): 1993-2001.doi: 10.16420/j.issn.0513-353x.2016-0889

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Studies on Genetic Diversity of Cymbidium kanran Populations from the Main Mountains in Jiangxi Province Based on ISSR Marker

JIANG Yawen,SUN Xiaoqin,LUO Huolin,YANG Boyun*,and XIONG Dongjin*   

  1. (School of Life Sciences,Nanchang University,Key Laboratory of Plant Resources in Jiangxi Province,Nanchang 330031,China)
  • Online:2017-10-25 Published:2017-10-25

Abstract: This study used ISSR molecular marker to analyze the genetic diversity and genetic structure of 12 Cymbidium kanran populations from the main mountains in Jiangxi Province. The results showed that 123 bands were amplified from 185 individuals from 12 primers,of which 97 bands were polymorphic,the percentage of polymorphic bands was 78.9%. The genetic diversity of 12 populations at species level was higher(PPB = 78.9%,Na = 1.7967,Ne = 1.4461,He = 0.2649,I = 0.3995),while the genetic diversity at population level was lower(PPB = 43.97%,Na = 1.4397,Ne = 1.2478,He = 0.1462,I = 0.2204). The genetic differentiation coefficient(GST)was 0.4415,gene flow(Nm)was 0.6325,these indicated that the genetic differentiation in the populations was higher than the genetic differentiation among the populations.Based on cluster analysis by UPGMA,12 C. kanran populations were classified into two groups,the first group included nine populations of ZX,WYS,WYSX,CYX,JA,YF,JGS,SW and SWX,the second group included three populations of SC,AY and CY,the result of cluster analysis showed no obvious correlation with the distribution of C. kanran populations according to the mountains. Mantel test showed that there was no significant correlation between genetic distance and geographical distance(r = 0.3791).

Key words: Cymbidium kanran, ISSR, genetic diversity, genetic structure

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