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ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (7): 1385-1392.doi: 10.16420/j.issn.0513-353x.2014-1146

• Research Notes • Previous Articles     Next Articles

Cloning and Expression Analysis of Thaumatin-like Protein Gene from Litchi chinenesis

WANG Shu-Jun, FENG Chao, WANG Ling-Yun, LI Huan-Ling, LIU Bao-Hua, WANG Jia-Bao   

  1. 1Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;2 College of Agriculture,Hainan University,Haikou 570228,China
  • Online:2015-07-25 Published:2015-07-25

Abstract: The full length of cDNA sequence and the genome sequence corresponding to the open reading frame(ORF)of a thaumatin-like protein gene(LcTLP,Accession No:JF682821)were obtained from‘Feizixiao’litchi(Litchi chinensis Sonn.‘Feizixaio’)by PCR method. The cDNA sequence was contained an ORF with 672 bp in length,encoding a polypeptide with 223 amino acid residues. There was no intron in LcTLP genome sequence. Quantitative real-time PCR results showed that the expression of LcTLP was the highest in the flower,followed by in the pericarp,and the lowest in the pulp. During the fruit development,the expression of LcTLP in the pericarp increased firstly,and then decreased. The expression of the gene in the pericarp of postharvested fruit was higher than that of developed fruit. The expression of LcTLP in the pericarp could be induced by Colletotrichum infection;However,it could be inhibited by water loss and low temperature.

Key words: litchi, thaumatin-like protein, gene cloning, gene expression

CLC Number: