Abstract: A novel member of class A1 heat shock transcription factors（Hsfs）in full length with 1 587 bp coding sequence encoding a protein of 528 amino acid residues with a molecular weight of 59.056 kD was isolated from the leaves of Lilium longiforum‘White Heaven’via RACE method. Deduced amino acid sequence analysis and phylogenic tree showed the gene named LlHsfA1 that had five critical domains and motifs，belonging to HsfA1 family and most closed to OsHsfA1a，was obviously different from each HsfA1 from other species. Moreover，it was constitutively expressed in roots，bulbs and leaves by qRT-PCR analysis under normal conditions. After 42 ℃ heat shock，LlHsfA1 expression level showed an alternate trend of‘rising and falling’，remarkably up-regulated by heat. The LlHsfA1-GFP fusion proteinswere observed to be located in the nucleus and cytoplasm.

Key words: Lilium longiforum, heat shock transcription factor, gene expression, subcellular location