Abstract: To understand gene response to fertilizer application，quantitative PCR and semiquantitative
PCR were used to analyze the expression of five genes（VvGHD，VvNiR，VvNR，VvGS and
VvAS）of‘Fujiminori’grape at different times with the fertilizer application. The results show that the
expression levels of five genes exhibit significant differences at different times after treatment，the overall
trend was first increased and then decreased；The highest expression level of VvGDH was in 48 h，VvNR
and VvNiR the highest expression level in 24 h，VvGS the highest expression level in 6 h，VvAS the highest
expression level in 2 h. The highest expression levels of five genes after ground fertilization in leaves were
delayed longer than those after foliar fertilizer application，and the overall expression level lower than
foliar fertilizer application；Response of five genes after ground fertilization in leaves was delayed longer
than the root，but the response intensity was significantly higher than corresponding genes in the root.

Key words: grapevine, nitrogen metabolism, gene clone, gene expression