Abstract: By isolated microspore culture techniques and artificial self-crossing method，DH population with 176 lines separately and F₂ population with 176 individuals were derived from a broccoli combination of two high-generation self-bred lines which were obviously different in phenotype. Average value，coefficient of variation（CV）and segregation region of 13 main agronomic characters were studied by analyzing the separation of agronomic characters and the genetic diversity. The 13 main agronomic traitswere generally controlled by multiple genes and inherited as quantitative trait loci（QTLs）. The positive and negative transgress genotypes could be generated in DH population and F₂ population.The average CV and genetic diversity index were similar between DH population and F₂ population.Therefore，the DH population could be used as breeding material to obtain breeding resources with wider segregation of character and accelerated the progress of parental purification and enhanced the breeding efficiency.

Key words: broccoli, DH population, F₂ population, character segregation, genetic diversity