Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 533-547.doi: 10.16420/j.issn.0513-353x.2021-0100

• Research Papers • Previous Articles     Next Articles

Creation of Fertility Restorer Materials for Ogura CMS Broccoli and the Study of Its Genetic Background

HUANG Jingjing1, LIU Wei2, LIU Yumei1, HAN Fengqing1, FANG Zhiyuan1, YANG Limei1, ZHUANG Mu1, ZHANG Yangyong1, LÜ Honghao1, WANG Yong1, JI Jialei1, LI Zhansheng1,*()   

  1. 1Key Laboratory of Horticultural Crop Biology and Germplasm Creation,Ministry of Agriculture,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
    2China Vegetable Biotechnology Shouguang Co.,Ltd,Shouguang,Shandong 262700,China
  • Received:2021-03-08 Revised:2021-11-08 Online:2022-03-25 Published:2022-03-25
  • Contact: LI Zhansheng


The Chinese kale material(14Y12)containing the Ogura CMS fertility restorer gene Rfo was used to introduce the Rfo restorer gene into six excellent Ogura CMS broccoli through cross breeding,and revealed its change characteristics and genetic background from the aspects of agronomic traits,cell level and genetic diversity. The results showed that the agronomic traits of Ogura CMS broccoli hybrids(F1 generation)were biased towards broccoli and genetic segregation was obvious. At the cellular level,there was a significant difference in the pollen viability of Rfo-transformed offsprings(P < 0.05),and the range was between 70% and 85%. There were abnormal phenomena of unequal division of chromosomes and loss of chromosomes in the late stage of meiosis. The pollen tubes of some Rfo-transformed offspring could be formed normally,while the pollen tube development of some Rfo-transformed offspring was blocked or abnormal. The ploidy identification showed that there were 12 diploids in Rfo-transformed offspring,accounting for 54.55% of the total,and 10 heterozygous ploidy between triploid and tetraploid,accounting for 45.45% of the total. The results of genetic analysis showed that at the genetic similarity coefficient of 0.50. Chinese kale 14Y12,broccoli 17B1253 and their Rfo-transformed offspring could be divided into two categories:A subgroup had 10 copies,with a genetic background biased toward Chinese kale;B subgroup had 22 copies,all of which are broccoli. The genetic similarity coefficient between Rfo-transformed offspring and Chinese kale was from 0.24 to 0.88,among which 19B809-8 had the farthest genetic background to Chinese kale,and the genetic similarity coefficient was 0.24,which could be used as the parent material for the fertility restoration of Ogura broccoli.

Key words: broccoli, Ogura CMS, fertility restorer, genetic background

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