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ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (1): 62-64.

• 研究报告 • Previous Articles     Next Articles

Study on Cryopreservation of in Vitro Cultured Cabernet France Shoot Tips

Zhao Yanhua and Wu Yongjie
  

  1. (Changli Institute of Pomology , Hebei Acadimic of Agriculture and Forestry Sciences Institute , Changli 066600)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-02-25 Published:2001-02-25

Abstract: Bud location , dehydration , prefreezing and subculture time were studied on grapevine Cabernet France shoot tip cryopreservation by the encapsulation-dehydration process. Best result (40 %) was obtained in the following
procedare : 70 select lateral buds after four months’subculture as explants , preculture them for 3 days , dehdrate for five hours then cool them in two steps and finally subculture them on standard culture medium.

Key words: Grape, Cryopreservation, Shoot tips

CLC Number: