Genetic Diversity of Crataegus pinnatifida Bge. as Evaluated by ISSR and SSR Markers

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (8): 1117-1124.

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Genetic Diversity of Crataegus pinnatifida Bge. as Evaluated by ISSR and SSR Markers

DAI hong-yan^1,2, GUO Xiu-wu^1,2, ZHANG Ye¹, LI Yuan-yuan^1,2, LI He¹, ZHOU Chuan-sheng², and ZHANG Zhi-hong^1,2*

（¹Team of Biotechnology and Genetic Improvement of Fruit Trees, Shenyang Agricultural University, Shenyang 110161, China; ²College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China）

Received:2008-02-03 Revised:2008-06-11 Online:2008-08-25 Published:2008-08-25
Contact: ZHANG Zhi-hong

Abstract

Abstract:

The DNA polymorphism of thirty-five hawthorn accessions (Crataegus pinnatifida Bge.) was analyzed using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers in the present study. Twelve RAPD primers generated a total of 110 clear and reproducible bands, of which 89 were polymorphic, with an average of 7.4 polymorphic fragments per primer. Thirteen ISSR primers generated a total of 110 clear and reproducible bands, of which 94 were polymorphic, with an average of 7.2 polymorphic fragments per primer. The dendrogram based on RAPD and ISSR data were constructed separately by using UPGMA analysis. The distance coefficient of the analyzed materials ranged from 0 to 0.62 and 0 to 0.64 for RAPD and ISSR markers, respectively. Our results indicated that the C. pinnatifida species has high level of genetic diversity.

Key words: Crataegus pinnatifida Bge., RAPD, ISSR, genetic diversity

CLC Number:

S 661.5

DAI hong-yan;GUO Xiu-wu;ZHANG Ye;LI Yuan-yuan;LI He;ZHOU Chuan-sheng;and ZHANG Zhi-hong;. Genetic Diversity of Crataegus pinnatifida Bge. as Evaluated by ISSR and SSR Markers[J]. ACTA HORTICULTURAE SINICA, 2008, 35(8): 1117-1124.

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Genetic Diversity of Crataegus pinnatifida Bge. as Evaluated by ISSR and SSR Markers

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