Genetic Variation and Fingerprinting of Torreya grandis Cultivars Detected by ISSR Markers

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (8): 1125-1130.

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Genetic Variation and Fingerprinting of Torreya grandis Cultivars Detected by ISSR Markers

DAI Zheng ^1,2, CHEN Li-geng ^1*, and TONG Pin-zhang³

(¹Department of Horticulture, Agriculture and Bio-technology College, Zhejiang University, Hangzhou 310029, China;²College of Horticulture, Qingdao Agricultural University,Qingdao,Shandong 266109, China; ³Forestry Bureau of Zhuji City, Zhejiang Province, Zhuji,Zhejiang 311800 ,China)

Received:2008-04-08 Revised:2008-06-11 Online:2008-08-25 Published:2008-08-25
Contact: CHEN Li-geng

Abstract

Abstract:

Inter-simple sequence repeat (ISSR) was used to determine the genomic DNA variations and relationships in Torreya grandis Fort.. Seventeen individuals including 9 cultivars and 8 elite clones were used in ISSR analysis. Of the 50 ISSR primers subjected to screening, 12 produced reproducible, polymorphic and disc- repant fragments. Using these primers, 104 DNA bands were amplified, of which 84.6% were polymorphic. The average number of DNA bands amplified by each primer was 11. DNA profiles based on ISSRs have revealed potential diagnostic for various individual accessions. A dendrogram generated using the Unweighted Pair Group Method with Arithmetic Average based on Nei's distance showed that the Torreya grandis cultivars were clustered into two groups. The first group include Dayuanfei, Mifei, Xuanwenfei, Liaoyafei, Zhimafei and Luoshuangfei, the second group include Xiangyafei, Xiaoyuanfei, Qiefei and the clone varieties of Torreya grandis 'Merrillii'.

Key words: Torreya grandis Fort., ISSR, genetic variation, genetic relationships

CLC Number:

S 664.5

DAI Zheng; CHEN Li-geng; and TONG Pin-zhang. Genetic Variation and Fingerprinting of Torreya grandis Cultivars Detected by ISSR Markers [J]. ACTA HORTICULTURAE SINICA, 2008, 35(8): 1125-1130.

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Genetic Variation and Fingerprinting of Torreya grandis Cultivars Detected by ISSR Markers

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