Cloning and Sequence Analysis of F3H Gene cDNA from Longan Embryo

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (11): 1581-1586.

• 果树 • Previous Articles Next Articles

Cloning and Sequence Analysis of F3H Gene cDNA from Longan Embryo

ZHANG Xi-juan^1,3;XU Hong-chuan^1,2;YOU Xiang-rong^1,2;LI Yan^1,2;CHEN Qing-xi^1,3;CHEN Wei^1,2*

（1Research Center for Proteomics, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China）

Received:2008-08-12 Revised:2008-10-13 Online:2008-11-25 Published:2008-11-25
Contact: CHEN Wei

Abstract

Abstract:

The protein of 'Lidongben' longan zygotic embryos during different development stages were separated and compared by IEF-SDS-PAGE technique. Flavanone 3-hydroxylase was one of differential proteins identified by MALDI-TOF-TOF/MS. Then F3H gene cDNA sequence was cloned from longan zygotic embryos using RT-PCR and RACE technique. A 1 404 bp full-length cDNA sequence was obtained. Analysis of F3H gene cDNA indicated that it encoded a peptide containing 365 amino acids. The nucleotide sequence comparison with the F3H gene of Citrus sinensis, Gossypium hirsutum and Pyrus communi showed that identity was all higher than 80%. The sequence was accepted and released by GenBank (Accession number:EF468104)。

Key words: longan, embryo, flavanone 3-hydroxylase, gene cloning, sequence analysis

CLC Number:

S 667.2

ZHANG Xi-juan;XU Hong-chuan;YOU Xiang-rong;LI Yan;CHEN Qing-xi;CHEN Wei;. Cloning and Sequence Analysis of F3H Gene cDNA from Longan Embryo[J]. ACTA HORTICULTURAE SINICA, 2008, 35(11): 1581-1586.

[1]	DENG Chaojun, XU Qizhi, JIANG Jimou, HU Wenshun, ZHENG Shaoquan, CHEN Xiuping, JIANG Fan, XU Jiahui, SU Wenbing, ZHANG Yaling, and HUANG Jingfeng. A New Longan Cultivar‘Chunxiang’with Rich Aroma [J]. Acta Horticulturae Sinica, 2022, 49(S2): 75-76.
[2]	DENG Chaojun, CHEN Xiuping, XU Qizhi, JIANG Jimou, ZHENG Shaoquan, HU Wenshun, JIANG Fan, XU Jiahui, SU Wenbing, ZHANG Yaling, and HUANG Jingfeng. A New Longan Cultivar‘Fuxiang’with Rich Aroma [J]. Acta Horticulturae Sinica, 2022, 49(S2): 77-78.
[3]	XU Xiaoping, CAO Qingying, CAI Roudi, GUAN Qingxu, ZHANG Zihao, CHEN Yukun, XU HAN, LIN Yuling, LAI Zhongxiong. Gene Cloning and Expression Analysis of miR408 and Its Target DlLAC12 in Globular Embryo Development and Abiotic Stress in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(9): 1866-1882.
[4]	WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe. Cloning and Functional Analysis of PgMYB111 Related to Anthocyanin Synthesis in Pomegranate [J]. Acta Horticulturae Sinica, 2022, 49(9): 1883-1894.
[5]	ZHANG Wanqing, ZHANG Hongxiao, LIAN Xiaofang, LI Yuying, GUO Lili, HOU Xiaogai. Analysis of DNA Methylation Related to Callus Differentiation and Rooting Induction of Paeonia ostii‘Fengdan’ [J]. Acta Horticulturae Sinica, 2022, 49(8): 1735-1746.
[6]	QIU Ziwen, LIU Linmin, LIN Yongsheng, LIN Xiaojie, LI Yongyu, WU Shaohua, YANG Chao. Cloning and Functional Analysis of the MbEGS Gene from Melaleuca bracteata [J]. Acta Horticulturae Sinica, 2022, 49(8): 1747-1760.
[7]	ZHENG Lin, WANG Shuai, LIU Yunuo, DU Meixia, PENG Aihong, HE Yongrui, CHEN Shanchun, ZOU Xiuping. Gene Cloning and Expression Analysis of NAC Gene in Citrus in Response to Huanglongbing [J]. Acta Horticulturae Sinica, 2022, 49(7): 1441-1457.
[8]	LIU Yaoyao, WU Yanyan, Shi Yan, MAO Tianyu, BAO Manzhu, ZHANG Junwei, ZHANG Jie. Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume [J]. Acta Horticulturae Sinica, 2022, 49(6): 1327-1338.
[9]	XIAO Xuechen, LIU Mengyu, JIANG Mengqi, CHEN Yan, XUE Xiaodong, ZHOU Chengzhe, WU Xingjian, WU Junnan, GUO Yinsheng, YEH Kaiwen, LAI Zhongxiong, LIN Yuling. Whole-genome Identification and Expression Analysis of SNAT,ASMT and COMT Families of Melatonin Synthesis Pathway in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(5): 1031-1046.
[10]	LI Shasha, YU Saisai, FU Yuheng, LUO Qiangwei, XU Yan, WANG Yuejin. The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes [J]. Acta Horticulturae Sinica, 2022, 49(4): 723-738.
[11]	JIA Jin, XU Yunlong, ZHOU Jiale, YANG Xiaofen, ZHONG Yonghui, ZENG Jiwu, HONG Ni, WANG Guoping, PENG Shu’ang, DING Fang. Detection and Identification of Huanglongbing Bacteria in‘Cocktail’Grapefruit [J]. Acta Horticulturae Sinica, 2022, 49(3): 590-596.
[12]	LIU Mengyu, JIANG Mengqi, CHEN Yan, ZHANG Shuting, XUE Xiaodong, XIAO Xuechen, LAI Zhongxiong, LIN Yuling. Genome-wide Identification and Expression Analysis of GDSL Esterase/Lipase Genes in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(3): 597-612.
[13]	SONG Mengfei, ZHA Gaohui, CHEN Jinfeng, LOU Qunfeng. Research Progress on Molecular Basis of Plant Architecture Related Traits in Cucumber [J]. Acta Horticulturae Sinica, 2022, 49(12): 2683-2702.
[14]	SHEN Xu, CHEN Xiaohui, ZHANG Jing, CHEN Rongzhu, XU Xiaoping, LI Xiaofei, JIANG Mengqi, LIU Pudong, NI Shanshan, LIN Yuling, LAI Zhongxiong. Evolutionary Dynamics Investigation and the Expression Analysis of Chromatin Remodeling Factor Snf2 Gene Family During Early Somatic Embryogenesis in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(1): 41-61.
[15]	LI Maofu, YANG Yuan, WANG Hua, FAN Youwei, SUN Pei, JIN Wanmei. Identification and Analysis of Self Incompatibility S-RNase in Rose [J]. Acta Horticulturae Sinica, 2022, 49(1): 157-165.

Cloning and Sequence Analysis of F3H Gene cDNA from Longan Embryo

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments