Abstract:

Improving keeping quality of strawberry fruit is a difficult problem. Applying antisense gene strategy to control ethylene receptor is a new feasible method. The antigene FaEtr2 was introduced into leaves of stawberry(Fragaria ananassa Duch.)with Agrobacterium tumefaciens，and 26 transgenic plants were selected under kanamycin. It was proved that anti- FaEtr2 was integrated with chromosome of transgenic plant，according to the results of PCR and Southern blot. Northern blot analysis indicated that FaEtr2 mRNA abundance was decresased in antisense strawberry plants. The ethylene production in transgenic strawberry significantly decreased. The results suggest that there is some intricate relation between FaEtr2 and the ripening of strawberry fruits. The anti-transgenic strawberry provide precious materials for the further research.

Key words: strawberry, ethylene receptors, genetic transformation