Cloning, Expression and Production of Polyclonal Antibodies of Peach PpLFY

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (11): 1573-1580.

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Cloning, Expression and Production of Polyclonal Antibodies of Peach PpLFY

AN Li-jun and LI Tian-hong*

（College of Agronomy and Biotechnology, China Agricultural University, Lab of Stress Physiology and Molecular Biology for Tree Fruits, A key Lab of Beijing Municipality, Beijing 100094, China）

Received:2008-07-01 Revised:2008-09-01 Online:2008-11-25 Published:2008-11-25
Contact: LI Tian-hong

Abstract

Abstract:

A FLO/LFY homologous gene named PpLEAFY（PpLFY）was cloned from 'Bayuecui' peach [Prunus persica (L.) Batsch.] axillary bud sampled in the flower induction period. (GenBank accession No. EF175869). The sequence of complete PpLFY cDNA gene was 1 314 bp, with an ORF 1 248 bp encoding 416 amino acids, which shared highly homology between 74.22% to 84.69% with other FLO/LFY protein from other species in the database. RT-PCR analysis showed that PpLFY gene expressed mainly in the flower induction time and early period of morphology differentiation and also the leaf and petal of peach. The prokaryotic expression systems was constructed and induced. The recombinant protein was purified to immunize the rabbit. SDS-PAGE analysis showed that the rPpLFY molecular weight was about 36 kD, EILSA analysis showed that the titer of polyclonal antibody was about 1:10 000.

Key words: peach, flowering gene, cloning, expression, polyclonal antibody

CLC Number:

S 662.1

AN Li-jun and LI Tian-hong. Cloning, Expression and Production of Polyclonal Antibodies of Peach PpLFY[J]. ACTA HORTICULTURAE SINICA, 2008, 35(11): 1573-1580.

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Cloning, Expression and Production of Polyclonal Antibodies of Peach PpLFY

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