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ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (1): 77-78.

• 研究报告 • Previous Articles     Next Articles

Effect of Phenolic Compounds on Efficiency of Genetic Transformation in Grape

Wu Di;Zhou Changmei;Zhu Yanming   

  1. (Life Science College , Northeast Agricultural University , Harbin 150030 , China)
  • Received:2002-01-21 Revised:2002-05-13 Online:2003-02-25 Published:2003-02-25

Abstract: In order to find out more efficient and cheaper phenolic compounds insteading of 3 , 5-dimetho xy4-hydro xyaceto phenone (AS) to improve efficiency of Agrobacterium-mediated transformation into grape , this research utilized Agrobacterium strain LBA4404 carrying pBI1301 with GUS and NPTII to transfer embryogenic callus of grape. The results showed that , compared with AS , trans-ferulic acid was better than AS , with the best concentration of 150 mg/ L ; 4-Hydroxy-3 , 5-dimethoxy-benzoesaure and erucic acid also had a significant efficiency of genetic transformation than control II (phenolic compound free) .

Key words: Grape, Agrobacterium, Efficiency of transformation, Phenolic compounds

CLC Number: