Radish Chromosome-specific ( dp ) RAPD Markers Screened from Raperadish Addition Lines

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (3): 767-770.

• 研究简报 • Previous Articles Next Articles

Radish Chromosome-specific ( dp ) RAPD Markers Screened from Raperadish Addition Lines

DING Yun-hua^1*
; BUDAHN Holger² ;JIAN Yuan-cai¹;PETERKA Herbert²

(¹ National Engineering Research Centre for Vegetable, Beijing 100097, China; ² Federal Centre for Breeding Research on Cultivated Plants, Quedlinburg D206484, Germany)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

Abstract

Abstract: In previous researches, a series of rape2radish addition lines with known additional radish
chromosomes and a linkage map of oil radish consisting of 9 group s have been developed. To study the assign
ment between the linkage group s and the chromosomes of radish, this work was carried out to screen radish
chromosome-specific molecularmarkers from rape-radish addition lines bymeans of ( dp) RAPD technology so
as to integrate radish chromosome markers into the linkage groups in further research. 576 decamer primers
and pair-primers were tested to identify molecularmarkers for radish chromosomes (A - I). As a result, 413
primers and pair-primerswere effectual to amp lify radish chromosome2specific ( dp) RAPD markers, the effec
tual primers (pair-primers) occup ied averagely 71.7%. This ratio in p rotocol of RAPD was 67.7% , and that
in dpRAPD was 73.2%. Altogether 899 ( dp) RAPD markers for all 9 chromosomes of radish were obtained,
including 354 RAPD markers and 545 dpRAPD markers. The number of markers for individual chromosome
varied from 41 for chromosome I to 160 for chromosome C.

Key words: Radish, Rape, Addition line, Chromosome, ( dp) RAPD

DING Yun-hua;BUDAHN Holger;JIAN Yuan-cai;PETERKA Herbert. Radish Chromosome-specific ( dp ) RAPD Markers Screened from Raperadish Addition Lines[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 767-770.

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Radish Chromosome-specific ( dp ) RAPD Markers Screened from Raperadish Addition Lines

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