Characterization of CmMYC2 in Formation of Green Color in Ray Florets of Chrysanthemum

doi:10.16420/j.issn.0513-353x.2021-0524

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2377-2387.doi: 10.16420/j.issn.0513-353x.2021-0524

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Characterization of CmMYC2 in Formation of Green Color in Ray Florets of Chrysanthemum

CHEN Sijia, WANG Huan, LI Ruirui, WANG Zhuoyi, LUO Jing(), WANG Caiyun

Key Laboratory for Biology of Horticultural Plants，Ministry of Education，College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China

Received:2022-03-09 Revised:2022-05-09 Online:2022-11-25 Published:2022-11-25
Contact: LUO Jing E-mail:ljcau@mail.hzau.edu.cn

Abstract

Abstract:

White Chrysanthemum vestitum and light green C. morifolium‘Chunxiao’，dark green C. morifolium‘Green anna’，as well as cut flower variety C. morifolium‘Lükou’were used to investigate the expression pattern and gene function of chlorophyll synthesis-related bHLH transcription factor gene CmMYC2. In addition，the regulation of chlorophyll synthesis-related genes by CmMYC2 was also studied. The results showed that the expression of CmMYC2 was gradually increased in ray florets and leaves of C. vestitum，C. morifolium‘Chunxiao’and‘Green Anna’，which was highly correlated with chlorophyll accumulation in these materials. Abscisic acid（ABA）treatment inhibited the accumulation of chlorophyll in ray florets and leaves of‘Lükou’and the expression of CmMYC2 and chlorophyll synthesis-related genes. The cluster heat map showed that there was a significant correlation between the expression pattern of CmMYC2 and chlorophyll synthesis-related genes in ray florets and leaves of ‘Lükou’under ABA treatment and in leaves of C. vestitum，C. morifolium‘Chunxiao’and ‘Green Anna’. Transient over-expression of CmMYC2 in leaves of‘Lükou’significantly promoted the accumulation of chlorophyll，and the chlorophyll synthesis-related genes，including HEMA1，CRD1，CHLG1 and PORA1 were notably induced by CmMYC2. The transient trans-activation assay in leaves of tobacco（Nicotiana benthamiana）showed the promoter activity of HEMA1 and CHLI1 was significantly enhanced by CmMYC2.

Key words: Chrysanthemum, flower color, chlorophyll, transcription factor, CmMYC2

CLC Number:

S682.11

CHEN Sijia, WANG Huan, LI Ruirui, WANG Zhuoyi, LUO Jing, WANG Caiyun. Characterization of CmMYC2 in Formation of Green Color in Ray Florets of Chrysanthemum[J]. Acta Horticulturae Sinica, 2022, 49(11): 2377-2387.

Figures/Tables 6

Fig. 1 Expression analysis of CmMYC2 and content of chlorophyll in Chrysanthemum vestitum and C. morifolium‘Chunxiao’and‘Green anna’ Expression of CmMYC2 in ray florets in C. vestitum and C. morifolium ‘Chunxiao’and‘Green anna’（A）;Content of chlorophyll（B）and expression of CmMYC2（C）in leaves of C. vestitum and C. morifolium‘Chunxiao’and‘Green anna’.

Fig. 2 Effects of ABA treatment on Chrysanthemum morifolium‘Lükou’phenotype（A，B），chlorophyll content（C），CmMYC2 and chlorophyll synthesis related gene expression（D）

Fig. 3 Phenotype（A），chlorophyll content（B）and relative expression of CmMYC2（C）in CmMYC2 overexpression and Super1300 control samples of Chrysanthemum morifolium‘Lükou’

Fig. 4 Expression of chlorophyll synthesis related genes in CmMYC2 overexpression and Super1300 control samples of Chrysanthemum morifolium‘Lükou’

Fig. 5 Analysis of chlorophyll cycling- and degradation-related gene expression in CmMYC2 overexpression and Super1300 control samples of Chrysanthemum morifolium‘Lükou’

Fig. 6 Regulation of promoter activity of chlorophyll synthesis gene by CmMYC2 A. Transient transactivation assays of HEMA1，CHLI1 and PORA1 promoters by CmMYC2;B. MYC binding sites in the promoters of HEMA1，CHLI1 and PORA1. Rectangles and triangles represent the binding sites on the + or - chains，respectively.

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Characterization of CmMYC2 in Formation of Green Color in Ray Florets of Chrysanthemum

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