Genome-wide Identification of Grape GRF Family and Expression Analysis

doi:10.16420/j.issn.0513-353x.2021-0533

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 995-1007.doi: 10.16420/j.issn.0513-353x.2021-0533

• Research Papers • Previous Articles Next Articles

Genome-wide Identification of Grape GRF Family and Expression Analysis

LIANG Chen, SUN Ruyi, XIANG Rui, SUN Yimeng, SHI Xiaoxin, DU Guoqiang(), WANG Li()

College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China

Received:2022-01-27 Revised:2022-03-28 Online:2022-05-25 Published:2022-05-25
Contact: DU Guoqiang,WANG Li E-mail:gdu@hebau.edu.cn;vivi@hebau.edu.cn

Abstract

Abstract:

The gene structure analysis,protein conserved motif identification,subcellular localization prediction,synteny analysis,phylogenetic analysis,and cis-acting elements analysis of Growth-regulating factor（GRF）family members were performed. Expression patterns of grape GRF genes in different plant parts and against different hormone applications were analyzed. Further expression patterns were compared during different stages of seed development in seeded and seedless cultivars. Eight grape GRF genes were identified and divided into four groups（A-D）. Seven of the genes were unevenly distributed on six chromosomes having and the number of amino acids was 213-604. All grape GRF proteins were predicted in the nucleus. Moreover,VvGRFs in the same clade showed conserved exon-intron structure and motif distribution patterns. All GRF proteins contained QLQ and WRC domains in the N-terminal region and at least one TQL or FFD domain in the C-terminal region. Synteny analysis showed that VvGRF3 and VvGRF4 were segmentally duplicated. Various cis-acting elements related to growth and development,hormones response,and stresses were found in the promoter region of VvGRF genes. Expression analysis illustrated that most of the VvGRFs were highly expressed in vegetative organs i.e. leaves. However,VvGRF2 was highly expressed in reproductive organs like flowers and fruits. The expression of VvGRF3 and VvGRF6 was significantly higher during seed development in seedless grapes as compared to seeded grapes,while VvGRF8 showed high expression in seeded grapes. The expression of VvGRF genes was induced against GA₃ and IAA treatments,and most of the VvGRFs were down-regulated at 0.5 or 1 h.

Key words: grape, GRF, family evolution, seed development, GA₃, IAA, expression analysis

CLC Number:

S663.1

LIANG Chen, SUN Ruyi, XIANG Rui, SUN Yimeng, SHI Xiaoxin, DU Guoqiang, WANG Li. Genome-wide Identification of Grape GRF Family and Expression Analysis[J]. Acta Horticulturae Sinica, 2022, 49(5): 995-1007.

Figures/Tables 11

References 41

[1]	Ai G, Zhang D, Huang R, Zhang S, Li W, Ahiakpa J K, Zhang J. 2020. Genome-wide identification and molecular characterization of the growth-regulating factors-interacting factor gene family in tomato. Genes, 11 (12):1435. doi: 10.3390/genes11121435 URL
[2]	Bailey T L, Boden M, Buske F A, Frith M, Grant C E, Clementi L, Ren J, Li W W, Noble W S. 2009. MEME SUITE:tools for motif discovery and searching. Nucleic Acids Research, 37 (s2):W202-W208.
[3]	Cao Y, Han Y, Jin Q, Lin Y, Cai Y. 2016. Comparative genomic analysis of the GRF genes in Chinese pear(Pyrus bretschneideri Rehd),poplar(Populous),grape(Vitis vinifera),Arabidopsis and rice(Oryza sativa). Frontiers in Plant Science, 7:1750.
[4]	Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools:an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13 (8):1194-1202. doi: 10.1016/j.molp.2020.06.009 URL
[5]	Choi D, Kim J H, Kende H. 2004. Whole genome analysis of the OsGRF gene family encoding plant-specific putative transcription activators in rice(Oryza sativa L.). Plant and Cell Physiology, 45 (7):897-904. doi: 10.1093/pcp/pch098 URL
[6]	Horiguchi G, Kim G T, Tsukaya H. 2005. The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana. Plant Journal, 43 (1):68-78. pmid: 15960617
[7]	Howe E A, Sinha R, Schlauch D, Quackenbush J. 2011. RNA-Seq analysis in MeV. Bioinformatics, 27 (22):3209-3210. doi: 10.1093/bioinformatics/btr490 URL
[8]	Hu B, Jin J, Guo A Y, Zhang H, Luo J, Gao G. 2015. GSDS 2.0:an upgraded gene feature visualization server. Bioinformatics, 31 (8):1296-1297. doi: 10.1093/bioinformatics/btu817 URL
[9]	Khatun K, Robin A H K, Park J I, Nath U K, Kim C K, Lim K B, Nou I S, Chung M Y. 2017. Molecular characterization and expression profiling of tomato GRF transcription factor family genes in response to abiotic stresses and phytohormones. International Journal of Molecular Sciences, 18 (5):1056. doi: 10.3390/ijms18051056 URL
[10]	Kim J H, Choi D, Kende H. 2003. The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis. Plant Journal, 36 (1):94-104. doi: 10.1046/j.1365-313X.2003.01862.x URL
[11]	Kim J H, Kende H. 2004. A transcriptional coactivator,AtGIF1,is involved in regulating leaf growth and morphology in Arabidopsis. Proceedings of the National Academy of Sciences, 101 (36):13374-13379.
[12]	Kim J H, Lee B H. 2006. Growth-regulating factor 4 of Arabidopsis thaliana is required for development of leaves,cotyledons,and shoot apical meristem. Journal of Plant Biology, 49 (6):463-468. doi: 10.1007/BF03031127 URL
[13]	Kim J S, Mizoi J, Kidokoro S, Maruyama K, Nakajima J, Nakashima K, Mitsuda N, Takiguchi Y, Ohme-Takagi M, Kondou Y, Yoshizumi T, Matsui M, Shinozaki K, Yamaguchi-Shinozaki K. 2012. Arabidopsis GROWTH-REGULATING FACTOR7 functions as a transcriptional repressor of abscisic acid- and osmotic stress-responsive genes,including DREB2A. Plant Cell, 24 (8):3393-3405. doi: 10.1105/tpc.112.100933 URL
[14]	Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, Jones S J, Marra M A. 2009. Circos:an information aesthetic for comparative genomics. Genome Research, 19 (9):1639-1645. doi: 10.1101/gr.092759.109 pmid: 19541911
[15]	Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X:molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35 (6):1547-1549. doi: 10.1093/molbev/msy096 URL
[16]	Lee T H, Tang H, Wang X, Paterson A H. 2013. PGDD:a database of gene and genome duplication in plants. Nucleic Acids Research, 41:D1152-1158.
[17]	Lescot M, Déhais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouzé P, Rombauts S. 2002. PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 30 (1):325-327.
[18]	Li S, Gao F, Xie K, Zeng X, Cao Y, Zeng J, He Z, Ren Y, Li W, Deng Q, Wang S, Zheng A, Zhu J, Liu H, Wang L, Li P. 2016. The OsmiR396c-OsGRF4-OsGIF 1 regulatory module determines grain size and yield in rice. Plant Biotechnology Journal, 14 (11):2134-2146. doi: 10.1111/pbi.12569 URL
[19]	Liu Pudong, Zhang Shuting, Chen Xiaohui, Su Liyao, Yao Deheng, Li Hansheng, Zhang Zihao. 2020. Genomic identification and expression patterns of the longan GRF family. Chinese Journal of Applied and Environmental Biology, 26 (2):236-245. (in Chinese)
	刘蒲东, 张舒婷, 陈晓慧, 苏立遥, 姚德恒, 李汉生, 张梓浩, 陈裕坤, 林玉玲, 赖钟雄. 2020. 龙眼GRF家族全基因组鉴定及表达模式. 应用与环境生物学报, 26 (2):236-245.
[20]	Liu X, Guo L X, Jin L F, Liu Y Z, Liu T, Fan Y H, Peng S A. 2016. Identification and transcript profiles of citrus growth-regulating factor genes involved in the regulation of leaf and fruit development. Molecular Biology Reports, 43 (10):1059-1067. doi: 10.1007/s11033-016-4048-1 URL
[21]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[22]	Omidbakhshfard M A, Proost S, Fujikura U, Mueller-Roeber B. 2015. Growth-regulating factors(GRFs):a small transcription factor family with important functions in plant biology. Molecular Plant, 8 (7):998-1010. doi: 10.1016/j.molp.2015.01.013 pmid: 25620770
[23]	Sun P, Zhang W, Wang Y, He Q, Shu F, Liu H, Wang J, Wang J, Yuan L, Deng H. 2016. OsGRF4 controls grain shape,panicle length and seed shattering in rice. Journal of Integrative Plant Biology, 58 (10):836-847. doi: 10.1111/jipb.12473 URL
[24]	van der Knaap E, Kim J H, Kende H. 2000. A novel gibberellin-induced gene from rice and its potential regulatory role in stem growth. Plant Physiology, 122 (3):695-704. pmid: 10712532
[25]	Wang F, Qiu N, Ding Q, Li J, Zhang Y, Li H, Gao J. 2014. Genome-wide identification and analysis of the growth-regulating factor family in Chinese cabbage(Brassica rapa L. ssp. pekinensis). BMC Genomics, 15:807. doi: 10.1186/1471-2164-15-807 URL
[26]	Wang L, Ahmad B, Liang C, Shi X X, Sun R Y, Zhang S L, Du G Q. 2020a. Bioinformatics and expression analysis of histone modification genes in grapevine predict their involvement in seed development,powdery mildew resistance,and hormonal signaling. BMC Plant Biology, 20 (1):412. doi: 10.1186/s12870-020-02618-7 URL
[27]	Wang L, Hu X, Jiao C, Li Z, Fei Z, Yan X, Liu C, Wang Y, Wang X. 2016. Transcriptome analyses of seed development in grape hybrids reveals a possible mechanism influencing seed size. BMC Genomics, 17 (1):898. pmid: 27829355
[28]	Wang L, Yin X, Cheng C, Wang H, Guo R, Xu X, Zhao J, Zheng Y, Wang X. 2015. Evolutionary and expression analysis of a MADS-box gene superfamily involved in ovule development of seeded and seedless grapevines. Molecular Genetics and Genomics, 290 (3):825-846. doi: 10.1007/s00438-014-0961-y pmid: 25429734
[29]	Wang L, Zhang S L, Zhang X M, Hu X Y, Guo C L, Wang X P, Song J Y. 2018. Evolutionary and expression analysis of Vitis vinifera OFP gene family. Plant Systematics and Evolution, 304 (8):995-1008. doi: 10.1007/s00606-018-1528-x URL
[30]	Wang J N, Zhou H J, Zhao Y Q, Sun P B, Tang F, Song X Q, Lu M Z. 2020b. Characterization of poplar growth regulating factors and analysis of their function in leaf size control. BMC Plant Biology, 20:509. doi: 10.1186/s12870-020-02699-4 URL
[31]	Wang Xiaofang, Chang Qinxiang, Wang Shufang, Yan Zhao, Li Xuyan, Gary Gao, Ji Wei. 2021. Bioinformatics analysis of GRF gene family in grape. Molecular Plant Breeding, 19 (18):5975-5983. (in Chinese)
	王晓芳, 昌秦湘, 王淑芳, 闫钊, 李戌彦, Gary Gao, 纪薇. 2021. 葡萄GRF基因家族的生物信息学分析. 分子植物育种, 19 (18):5975-5983.
[32]	Wei Yanhong, Liu Zhen, Li Ke, Meng Yuan, Wang Hui, Mao Jiangping, Ma Doudou, Li Shaohuan, Ma Juanjuan, Lu Xian, Zhang Dong. 2020. Genome-wide identification and expression analysis of miR396 family during adventitious root development in apple. Acta Horticulturae Sinica, 47 (7):1237-1252. (in Chinese)
	韦燕红, 刘桢, 李珂, 孟媛, 汪蕙, 毛江萍, 马豆豆, 李少欢, 马娟娟, 卢显, 张东. 2020. 苹果miR396 家族鉴定及在不定根发育过程中的表达分析. 园艺学报, 47 (7):1237-1252.
[33]	Wu L, Zhang D, Xue M, Qian J, He Y, Wang S. 2014. Overexpression of the maize GRF10,an endogenous truncated growth-regulating factor protein,leads to reduction in leaf size and plant height. Journal of Integrative Plant Biology, 56 (11):1053-1063. doi: 10.1111/jipb.12220 URL
[34]	Wu Z J, Wang W L, Zhuang J. 2017. Developmental processes and responses to hormonal stimuli in tea plant(Camellia sinensis)leaves are controlled by GRF and GIF gene families. Functional & Integrative Genomics, 17 (5):503-512.
[35]	Zhang D F, Li B, Jia G Q, Zhang T F, Dai J R, Li J S, Wang S C. 2008. Isolation and characterization of genes encoding GRF transcription factors and GIF transcriptional coactivators in maize(Zea mays L.). Plant Science, 175 (6):809-817. doi: 10.1016/j.plantsci.2008.08.002 URL
[36]	Zhang J, Li Z, Jin J, Xie X, Zhang H, Chen Q, Luo Z, Yang J. 2018. Genome-wide identification and analysis of the growth-regulating factor family in tobacco(Nicotiana tabacum). Gene, 639:117-127. doi: 10.1016/j.gene.2017.09.070 URL
[37]	Zhang S, Wang L, Sun X, Li Y, Yao J, van Nocker S, Wang X. 2019. Genome-wide analysis of the YABBY gene family in grapevine and functional characterization of VvYABBY4. Frontiers in Plant Science, 10:1207. doi: 10.3389/fpls.2019.01207 URL
[38]	Zhao Huixia, Li Fengxia, Guo Rui, Chen Chanyou. 2020. Genome-wide characterization and expression analysis of GRF gene family in Capsicum. Acta Horticulturae Sinica, 47 (11):2145-2160. (in Chinese)
	赵慧霞, 李凤霞, 郭瑞, 陈禅友. 2020. 辣椒属GRF基因家族全基因组鉴定和表达分析. 园艺学报, 47 (11):2145-2160.
[39]	Zheng L W, Ma J J, Song C H, Zhang L Z, Gao C, Zhang D, An N, Mao J P, Han M Y. 2018. Genome-wide identification and expression analysis of GRF genes regulating apple tree architecture. Tree Genetics & Genomes, 14 (4):54.
[40]	Zhou H J, Song X Q, Wei K L, Zhao Y Q, Jiang C, Wang J N, Tang F, Lu M Z. 2019. Growth-regulating factor 15 is required for leaf size control in Populus. Tree Physiology, 39 (3):381-390. doi: 10.1093/treephys/tpy107 URL
[41]	Zhu Y X, Li Y D, Zhang S L, Zhang X M, Yao J, Luo Q W, Sun F, Wang X P. 2019. Genome-wide identification and expression analysis reveal the potential function of ethylene responsive factor gene family in response to Botrytis cinerea infection and ovule development in grapes(Vitis vinifera L.). Plant Biology, 21 (4):571-584. doi: 10.1111/plb.12943 pmid: 30468551

基因Gene	上游引物（5'-3'）Forward primer	下游引物（5'-3'）Reverse primer
VvGRF1	GGGCTTCCAGTGCCTTATC	TTCCCATCCGTTCTTCTACAT
VvGRF2	CTGGTGGGACTCATTTCGC	AGGTTGCCTGGCTGTTATTG
VvGRF3	GCCTCAATGACTTCTGAAACCT	CGATGATGCCTGTAATCCCTAT
VvGRF4	CAGAAAGCCTGTGGAAGTTATG	TGTAGCAAGAAGGGTGACGAT
VvGRF5	CGCCTTTGGACAGCACAT	CGAGACTGACCCTCCTGTTTC
VvGRF6	TCGTTCTATCTTCAACCTCCGT	AGTACTTCTGATTGAGTGCCACAT
VvGRF7	GAAGCCCTTGATTCCGTTG	TCTCACAATACTTCTGATCGGC
VvGRF8	CGCCACTCAGGCTATCTCG	AAGCAGGCTCCCATGTTGT
EF1-α	AGGAGGCAGCCAACTTCACC	CAAACCCTGCATCACCATTC

基因Gene	上游引物（5'-3'）Forward primer	下游引物（5'-3'）Reverse primer
VvGRF1	GGGCTTCCAGTGCCTTATC	TTCCCATCCGTTCTTCTACAT
VvGRF2	CTGGTGGGACTCATTTCGC	AGGTTGCCTGGCTGTTATTG
VvGRF3	GCCTCAATGACTTCTGAAACCT	CGATGATGCCTGTAATCCCTAT
VvGRF4	CAGAAAGCCTGTGGAAGTTATG	TGTAGCAAGAAGGGTGACGAT
VvGRF5	CGCCTTTGGACAGCACAT	CGAGACTGACCCTCCTGTTTC
VvGRF6	TCGTTCTATCTTCAACCTCCGT	AGTACTTCTGATTGAGTGCCACAT
VvGRF7	GAAGCCCTTGATTCCGTTG	TCTCACAATACTTCTGATCGGC
VvGRF8	CGCCACTCAGGCTATCTCG	AAGCAGGCTCCCATGTTGT
EF1-α	AGGAGGCAGCCAACTTCACC	CAAACCCTGCATCACCATTC

基因 Gene	登录号 Accession No.	基因ID Gene locus ID	VCost.v3 ID	起始与终止位点/bp Start and end position	CDS/bp	ORF/ aa	亚细胞定位 Subcellular localization
VvGRF1	XP_002281639.1	GSVIVT01019913001	Vitvi02g00449	4 421 518-4 424 601	639	213	细胞核Nucleus
VvGRF2	XP_002277233.3	GSVIVT01033800001	Vitvi08g01498	17 725 858-17 728 519	1 176	392	细胞核Nucleus
VvGRF3	XP_010654438.1	GSVIVT01016762001	Vitvi09g00107	1 135 208-1 137 036	1 092	364	细胞核Nucleus
VvGRF4	XP_010656126.2	GSVIVT01015095001	Vitvi11g00092	1 000 359-1 001 747	1 164	388	细胞核Nucleus
VvGRF5	XP_002270427.2	GSVIVT01024326001	Vitvi16g00073	975 755-979 958	1 122	374	细胞核Nucleus
VvGRF6	XP_002271355.3	GSVIVT01038629001	Vitvi16g01354	21 346 554-21 349 482	1 812	604	细胞核Nucleus
VvGRF7	CBI19365.3	GSVIVT01009299001	Vitvi18g00623	7 086 633-7 089 964	1 428	476	细胞核Nucleus
VvGRF8	CBI33069.3	GSVIVT01007165001	Vitvi07g01551	30 596 800-30 600 327	1 674	558	细胞核Nucleus

基因 Gene	登录号 Accession No.	基因ID Gene locus ID	VCost.v3 ID	起始与终止位点/bp Start and end position	CDS/bp	ORF/ aa	亚细胞定位 Subcellular localization
VvGRF1	XP_002281639.1	GSVIVT01019913001	Vitvi02g00449	4 421 518-4 424 601	639	213	细胞核Nucleus
VvGRF2	XP_002277233.3	GSVIVT01033800001	Vitvi08g01498	17 725 858-17 728 519	1 176	392	细胞核Nucleus
VvGRF3	XP_010654438.1	GSVIVT01016762001	Vitvi09g00107	1 135 208-1 137 036	1 092	364	细胞核Nucleus
VvGRF4	XP_010656126.2	GSVIVT01015095001	Vitvi11g00092	1 000 359-1 001 747	1 164	388	细胞核Nucleus
VvGRF5	XP_002270427.2	GSVIVT01024326001	Vitvi16g00073	975 755-979 958	1 122	374	细胞核Nucleus
VvGRF6	XP_002271355.3	GSVIVT01038629001	Vitvi16g01354	21 346 554-21 349 482	1 812	604	细胞核Nucleus
VvGRF7	CBI19365.3	GSVIVT01009299001	Vitvi18g00623	7 086 633-7 089 964	1 428	476	细胞核Nucleus
VvGRF8	CBI33069.3	GSVIVT01007165001	Vitvi07g01551	30 596 800-30 600 327	1 674	558	细胞核Nucleus

Genome-wide Identification of Grape GRF Family and Expression Analysis

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