https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 477-486.doi: 10.16420/j.issn.0513-353x.2020-0304

• Research Papers • Previous Articles     Next Articles

Efficacy of Low Temperature and Mineral Oil Covering in Prolonging Subculture Interval During Grape Germplasm Preservation in Vitro

CHENG Xiaohua1, XU Zhiwei1,2, WANG Li1, SHI Xiaoxin1,**(), DU Guoqiang1,**()   

  1. 1College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China
    2Wuyi Hongda High School,Hengshui,Hebei 053400,China
  • Received:2020-09-24 Online:2021-03-25 Published:2021-04-02
  • Contact: SHI Xiaoxin,DU Guoqiang E-mail:shixx@hebau.edu.cn;gdu@hebau.edu.cn

Abstract:

Fourteen grape cultivars were used as plant materials to reveal the influences of low culture temperature and mineral oil covering on medium of plantlets in vitro. The results showed that the low temperature could significantly prolong the subculture period,but the optimal low temperature varied according to the different cultivars. Plantlets cultured in the low temperature could resume growth well after being transferred to routine temperature culture. When the survival rate of 30%-40% was a concern,the optimal temperature was 9 ℃ for‘Sweet Scarlet’,‘Melissa’,‘Summer Royal'and‘Crimson Seedless’cultivars,and the subculture period could reach more than 850 days,and maximum 1 060 days for certain cultivar;the optimal temperature was 12-15 ℃ for‘Kyoho’,‘Jumeigui’,‘Autumn Royal’,‘Flame Seedless’,‘Summer Black’,‘Princess’,‘Lilit’,‘Cabernet Sauvignon’,‘Manicure Finger'and‘Wink’,and the subculture period could last 350 to 600 days. Prior to the low temperature culture,the plantlets needed to be cultured under routine condition for a period of time(preculture). The duration depended on the culture temperature and the varieties,and it was usually 7 to 21 days. By inoculating shoots with single bud and without leaf,as well as adding 6-8 cm mineral oil on the top of the medium,the subculture period could be up to 420 days in routine temperature culture condition while the control was 150 days. The plantlets resumed growth after the mineral oil was removed.

Key words: grape, germplasm preservation, plantlet in vitro, retard growth, subculture interval, low temperature, preculture, mineral oil covering

CLC Number: