Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and
Metabolome

doi:10.16420/j.issn.0513-353x.2024-0376

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 933-946.doi: 10.16420/j.issn.0513-353x.2024-0376

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and Metabolome

SHAO Yifan¹，ZHU Baoqing¹，WANG Tongxin¹，LIAO Jianhe²，WU Fanhua³，YANG Siyi¹，FENG Jianhang¹，and YU Xudong^1,*(yuxd@hainanu.edu.cn)

¹Key Laboratory of Genetics and Germplasm Innovation of Tropical Forest Trees and Ornamental Plants（Ministry of Education）；Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province，School of Tropical Agriculture and Forestry, Hainan University，Danzhou，Hainan 571737，China ²School of Materials Science and Engineering，Hainan University，Haikou 570228，China ³College of life sciences，Hainan University，Haikou 570228，China

Received:2025-01-08 Revised:2025-02-20 Online:2025-04-25 Published:2025-04-25
Contact: YU Xudong

Abstract

Abstract: Key regulatory genes that create prickles in Bombax ceiba were explored via phytohormone assays，transcriptomics，and metabolomics by use of the stem bark from the upper less prickly zone and lower prickly zone. The phytohormone assay revealed that abscisic acid，jasmonic acid，and salicylic acid were higher in the less prickly zone than in the prickly zone，whereas gibberellin was higher in the more prickly zone than in the less prickly zone. Transcriptome sequencing revealed that in the growth hormone biosynthesis pathway，the expression of Aux/IAA protein genes and growth hormone response factor ARF genes was higher in the prickly zone than in the less prickly zone. In the cytokinin biosynthesis pathway，the expression of B-ARR genes was higher in the prickly zone than in the less prickly zone whereas，in the gibberellin biosynthesis pathway，the expression of the GID1，DELLA，and TF genes was higher in the less prickly zone than in the prickly area. Furthermore，the expression of the JAR1，JAZ，and MYC2 genes was higher in the less prickly zone than in the prickly zone in the jasmonic acid biosynthesis pathway. Overall，13 key genes（BRI1，BZR1/2，AUX1，AUX/IAA，ARF，DELLA，JAR1，TGA，PR-1，SIMKK，MYB62，MYB123，and WRKY28） that affected the development of cottonwood prickles were verified by qRT-PCR，which were similar to the results of high-throughput sequencing；thus，supporting the reliability of the RNA-Seq results. Metabolomics screened 2 187 metabolites and 92 differential metabolites and identified that the CCR and COMT genes in the phenylpropanoid biosynthesis pathway as well as FLS genes in the flavonoid biosynthesis pathway, were related to the formation of cottonwood prickles. In addition，the metabolites（2S）-2-isopropylmalic acid and （2R,3S）-3-isopropyl malate in the valine，leucine，and isoleucine metabolic pathways and quercetin in the flavonoid biosynthesis pathway are involved in cottonwood prickle formation.

Key words: Bombax ceiba, prickle, plant hormones, transcriptome, metabolome

CLC Number:

S 685.99

SHAO Yifan, ZHU Baoqing, WANG Tongxin, LIAO Jianhe, WU Fanhua, YANG Siyi, FENG Jianhang, YU Xudong. Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and Metabolome[J]. Acta Horticulturae Sinica, 2025, 52(4): 933-946.

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Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and Metabolome

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