Growth，Development and Transcriptome Analysis of Tomato Cultivars with Different Low Light Tolerances Under Low Light Condition

doi:10.16420/j.issn.0513-353x.2023-0775

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (2): 335-345.doi: 10.16420/j.issn.0513-353x.2023-0775

• Cultivation·Physiology&Biochemistry • Previous Articles Next Articles

Growth，Development and Transcriptome Analysis of Tomato Cultivars with Different Low Light Tolerances Under Low Light Condition

WANG Hong, MIAO Chen, DING Xiaotao, SHEN Haibin^*(), ZHU Weimin

Shanghai Key Laboratory of Protected Horticultural Technology，Horticultural Research Institute，Shanghai Academy of Agricultural Sciences，Shanghai 201403，China

Received:2023-10-07 Revised:2023-12-11 Online:2024-02-25 Published:2024-02-26
Contact: SHEN Haibin

Abstract

Abstract:

In this study，low-light sensitive cultivar‘K20’and low-light tolerant cultivar‘Shenfen 16’were selected as materials. The effects of low light on plant height，stem diameter，internode length，numbers of leaves，antioxidant enzyme activity，MDA content and RNA-seq data were analyzed. The results showed that low light inhibited the growth of tomato plants，accompanied with an increase in MDA content and decreases in antioxidant enzyme activities，but the reductions in ‘Shenfen 16’were smaller than those in‘K20’. Furthermore，898 differentially expressed genes（DEGs）were identified in‘K20’and 2 849 DEGs were obtained in‘Shenfen 16’under low light stress. The up-regulated pathway of Kyoto Encyclopedia of Genes and Genomes（KEGG）enrichments in‘K20’were‘protein processing in endoplasmic reticulum’and‘ribosome biogenesis in eukaryote’，whereas the largest amount of DEGs in‘Shenfen 16’were annotated with‘plant hormone signal transduction’and followed by‘MAPK signaling pathway’. These results suggested that low-light tolerant cultivar may increase its adaptation to low light by regulating the biosynthesis of hormones. In summary，low light inhibited the growth of tomato plants，but low light-tolerant cultivar may increase its adaptability to low light by regulating the synthesis of various hormone such as IAA and increasing the activities of antioxidant enzymes.

Key words: Solanum lycopersicum, stress, low light, hormone, transcriptome analysis

WANG Hong, MIAO Chen, DING Xiaotao, SHEN Haibin, ZHU Weimin. Growth，Development and Transcriptome Analysis of Tomato Cultivars with Different Low Light Tolerances Under Low Light Condition[J]. Acta Horticulturae Sinica, 2024, 51(2): 335-345.

Figures/Tables 7

Fig. 1 The dynamic changes in plant height，stem diameter，internode length and leaf number of different tomato cultivars under low light stress

Fig. 2 Effects of low light stress on fresh weightand dry weight in different tomato cultivars Different lowercase letters indicate significant statistical differences（P < 0.05）. The same below.

Fig. 3 Effects of low light stress on the content of MDA in different tomato cultivars

Fig. 4 Effects of low light stress on antioxidant enzyme activities in different tomato cultivars

Fig. 5 The number of differentially expressed genes in different tomato cultivars under low light stress

Fig. 6 Effects of low light stress on plant hormone signal transduction pathway in‘Shenfen 16’tomato Red indicates up-regulated genes，green indicates down-regulated genes，and yellow indicates non-differential genes.

Fig. 7 Effects of low light stress on plant hormone signal transduction pathway in‘K20’tomato Red indicates up-regulated genes，green indicates down-regulated genes，and yellow indicates non-differential genes.

References 36

[1]	Bawa G, Feng L, Chen G, Chen H, Hu Y, Pu T, Cheng Y, Shi J, Xiao T, Zhou W, Yong T, Sun X, Yang F, Yang W, Wang X. 2020. Gibberellins and auxin regulate soybean hypocotyl elongation under low light and high-temperature interaction. Physiologia Plantarum, 170 (3):345-356.
[2]	Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72:248-254. pmid: 942051
[3]	Cakmak I, Marschner H. 1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase,and glutathione reductase in bean leaves 1. Plant Physiology, 98:1222-1227. doi: 10.1104/pp.98.4.1222 pmid: 16668779
[4]	Chen N, Shao Q, Lu Q, Li X, Gao Y. 2022. Transcriptome analysis reveals differential transcription in tomato(Solanum lycopersicum)following inoculation with Ralstonia solanacearum. Scientific Reports, 12:22137. doi: 10.1038/s41598-022-26693-y pmid: 36550145
[5]	Cheng Ya-jiao, Shen Jun-xu, Wang Zhong-lin, Fan Yuan-fang, Chen Si-yu, Li Ze-lin, Liu Qin-lin, Li Zhong-chuan, Yang Feng, Yang Wen-yu. 2018. Effects of light intensity and light quality on morphological and photosynthetic characteristics of soybean seedlings. Scientia Agricultura Sinica, 51 (14):2655-2663. (in Chinese) doi: 10.3864/j.issn.0578-1752.2018.14.003
	程亚娇, 谌俊旭, 王仲林, 范元芳, 陈思宇, 李泽林, 刘沁林, 李中川, 杨峰, 杨文钰. 2018. 光强和光质对大豆幼苗形态及光合特性的影响. 中国农业科学, 51 (14):2655-2663. doi: 10.3864/j.issn.0578-1752.2018.14.003
[6]	Dai Y J, Shen Z G, Liu Y, Wang L L, Hannaway D. 2009. Effects of shade treatments on the photosynthetic capacity,chlorophyll fluorescence,and chlorophyll content of Tetrastigma hemsleyanum Diels et Gilg. Environmental and Experimental Botany, 65:177-182.
[7]	Deng Y M, Li C C, Shao Q S, Ye X Q, She J M. 2012. Differential responses of double petal and multi petal jasmine to shading:I. photosynthetic characteristics and chloroplast ultra structure. Plant Physiology and Biochemistry, 55:93-102.
[8]	Ding J, Zhao J, Pan T, Xi L, Zhang J, Zou Z. 2019. Comparative transcriptome analysis of gene expression patterns in tomato under dynamic light conditions. Genes, 10 (9):669.
[9]	Dong C, Fu Y, Liu G, Liu H. 2014. Light intensity effects on the growth,photosynthetic characteristics,antioxidant capacity,yield and quality of wheat(Triticum aestivum L.)at different growth stages in BLS. Advances in Space Research, 53:1557-1566.
[10]	Fiorucci A S, Fankhauser C. 2017. Plant strategies for enhancing access to sunlight. Current Biology, 27:931-940.
[11]	Giannopolitis C N, Ries S K. 1977. Superoxide dismutases:I. occurrence in higher plants. Plant Physiology, 59:309-314. doi: 10.1104/pp.59.2.309 pmid: 16659839
[12]	Hodges D M, Delong J M, Forney C F, Prange R K. 1999. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207:604-611.
[13]	Knapp S, Peralta I E. 2016. The tomato(Solanum lycopersicum L.,Solanaceae)and its botanical relatives. The Tomato Genome. Berlin, Heidelberg:Springer:7-21.
[14]	Kozuka T, Kobayashi J, Horiguchi G, Demura T, Sakakibara H, Tsukaya H, Nagatani A. 2010. Involvement of auxin and brassinosteroid in the regulation of petiole elongation under the shade. Plant Physiology, 153 (4):1608-1618. doi: 10.1104/pp.110.156802 pmid: 20538889
[15]	Kurepin L V, Emery R J N, Pharis R P, Reid D M. 2007. The interaction of light quality and irradiance with gibberellins,cytokinins,and auxin in regulating growth of Helianthus annuus hypocotyls. Plant,Cell and Environment, 30:147-155.
[16]	Li D, Yu F, Zhang Y, Hu K, Dai D, Song S, Sheng Y. 2022. Integrative analysis of different low-light-tolerant cucumber lines in response to low-light stress. Frontiers in Plant Science, 13:1093859.
[17]	Li Y F, Fan Y, Ma Y, Zhang Z, Yue H B, Wang L J, Li J, Jiao Y. 2017. Effects of exogenous gamma-aminobutyric acid(GABA)on photosynthesis and antioxidant system in pepper(Capsicum annuum L.) seedlings under low light stress. Journal of Plant Growth Regulation, 36:436-449.
[18]	Liu Q H, Wu X, Chen B C, Ma J Q, Gao J. 2014. Effects of low light on agronomic and physiological characteristics of rice including grain yield and quality. Rice Science, 2:243-251.
[19]	Liu W, Liu K, Chen D, Zhang Z, Li B, El-Mogy M M, Tian S, Chen T. 2022. Solanum lycopersicum,a model plant for the studies in developmental biology,stress biology and food science. Foods, 11:1-15.
[20]	Lu T, Yu H, Li Q, Chai L, Jiang W. 2019. Improving plant growth and alleviating photosynthetic inhibition and oxidative stress from low-light stress with exogenous GR24 in tomato(Solanum lycopersicum L.)seedlings. Frontiers in Plant Science, 10:490.
[21]	Lv B, Yan Z, Tian H, Zhang X, Ding Z. 2019. Local auxin biosynthesis mediates plant growth and development. Trends in Plant Science, 24:6-9. doi: S1360-1385(18)30247-4 pmid: 30448230
[22]	Meng Xian-min, Ji Yan-hai, Sun Wang-wang, Wu Zhan-hui, Chu Zhao-sheng, Liu Ming-chi. 2021. Response of chloroplast ultrastructure and photosynthetic physiology of two tomato varieties to low light stress. Scientia Agricultura Sinica, 54 (5):1017-1028. (in Chinese) doi: 10.3864/j.issn.0578-1752.2021.05.013
	孟宪敏, 季延海, 孙旺旺, 武占会, 储昭胜, 刘明池. 2021. 两个番茄品种叶绿体超微结构及光合生理对弱光胁迫的响应. 中国农业科学, 54 (5):1017-1028. doi: 10.3864/j.issn.0578-1752.2021.05.013
[23]	Miao Chen. 2020. Response and evaluation of different tomato varieties to supplement lighting cultivation in facility[M. D. Dissertation]. Nanjing: Nanjing Agricultural University. (in Chinese)
	苗辰. 2020. 不同品种番茄对设施栽培补光的响应与评价[硕士论文]. 南京: 南京农业大学.
[24]	Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22:867-880.
[25]	Patra H K, Kar M, Mishra D. 1978. Catalase activity in leaves and cotyledons during plant development and senescence. Biochemie und Physiologie der Pflanzen, 172:385-390.
[26]	Ren M F, Liu S Z, Mao G L, Tang C Z, Gai P P, Guo X L, Zheng H B, Wang W Q, Tang Q Y. 2023. Simultaneous application of red and blue light regulate carbon and nitrogen metabolism,induces antioxidant defense system and promote growth in rice seedlings under low light stress. International Journal of Molecular Sciences, 24 (13):10706.
[27]	Sekhar S, Panda D, Kumar J, Mohanty N, Biswal M, Baig M J, Kumar A, Umakanta N, Samantaray S, Pradhan S K, Shao B P, Swain P, Behera L. 2019. Comparative transcriptome profiling of low light tolerant and sensitive rice varieties induced by low light stress at active tillering stage. Scientific Reports, 9:5753. doi: 10.1038/s41598-019-42170-5 pmid: 30962576
[28]	Song Mengfei, Zha Gaohui, Chen Jinfeng, Lou Qunfeng. 2022. Research progress on molecular basis of plant architecture related traits in cucumber. Acta Horticulturae Sinica, 49 (12):2683-2702. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0674
	宋蒙飞, 查高辉, 陈劲枫, 娄群峰. 2022. 黄瓜株型性状分子基础研究进展. 园艺学报, 49 (12):2683-2702. doi: 10.16420/j.issn.0513-353x.2021-0674
[29]	Suzuki N, Koussevitzky S, Mittler R, Miller G. 2012. ROS and redox signalling in the response of plants to abiotic stress. Plant Cell and Environment, 35:259-270.
[30]	Tang Mingjia, Xu Jin, Lin Rui, Song Jianing, Yu Jingquan, Zhou Yanhong. 2022. Advances in physiological and molecular mechanism of tomato responses to light and temperature stress. Acta Horticulturae Sinica, 49 (10):2174-2188. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-0600
	唐明佳, 徐进, 林锐, 宋珈凝, 喻景权, 周艳虹. 2022. 番茄响应光温逆境的生理分子机制研究进展. 园艺学报, 49 (10):2174-2188. doi: 10.16420/j.issn.0513-353x.2022-0600
[31]	Tripodi P, Fig`as M R, Leteo F, Soler S, Díez M J, Campanelli G, Cardi T, Prohens J. 2022. Genotypic and environmental effects on morpho-physiological and agronomic performances of a tomato diversity panel in relation to nitrogen and water stress under organic farming. Frontier in Plant Science, 13:1-19.
[32]	Wang M, Jiang W J, Yu H J. 2010. Effects of exogenous epibrassinolide on photosynthetic characteristics in tomato(Lycopersicon esculentum Mill)seedlings under weak light stress. Journal of Agricultural and Food Chemistry, 58:3642-3645.
[33]	Xu G, Li L, Zhou J, Lyu D, Zhao D, Qin S. 2023. Comparison of transcriptome and metabolome analysis revealed differences in cold resistant metabolic pathways in different apple cultivars under low temperature stress. Horticultural Plant Journal, 9 (2):183-198.
[34]	Yang C, Li L. 2017. Hormonal regulation in shade avoidance. Frontiers in Plant Science, 8:1527. doi: 10.3389/fpls.2017.01527 pmid: 28928761
[35]	Zhang Q, Gong M, Xu X, Li H, Deng W. 2022. Roles of auxin in the growth,development,and stress tolerance of horticultural plants. Cell, 11:2761.
[36]	Zou Chang-ming, Wang Run-qing, Liu Ying, Zhang Xiao-hong, Tang Shan. 2015. Responses of photosynthesis and growth to weak light regime in four legume species. Chinese Journal of Plant Ecology, 39 (9):909-916. (in Chinese) doi: 10.17521/cjpe.2015.0087
	邹长明, 王允青, 刘英, 张晓红, 唐杉. 2015. 四种豆科作物的光合生理和生长发育对弱光的响应. 植物生态学报, 39 (9):909-916. doi: 10.17521/cjpe.2015.0087

Growth，Development and Transcriptome Analysis of Tomato Cultivars with Different Low Light Tolerances Under Low Light Condition

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 36

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Wenjing, SHI Shuo, ZHANG Hao, ZHANG Jiangyan, SONG Sihao, YANG Aizhen, SHEN Yuanyue, GUO Jiaxuan, and GAO Fan, . Effects of Exogenous Nitric Oxide and Putrescine on Drought Resistance of Strawberry Seedlings [J]. Acta Horticulturae Sinica, 2024, 51(9): 2131-2142.
[2]	GUO Qunyan, LIU Caixian, YU Qiuxiu, ZHANG Hua, ZHENG Meiting. Studies on the Physiological Indexes and Endogenous Hormones Variation During Flower Development in Michelia crassipes [J]. Acta Horticulturae Sinica, 2024, 51(8): 1881-1890.
[3]	WANG Jianzhao, GAO Yuan, DAO Mei, ZHANG Hongye, YANG Ziyun, CHEN Longqing, WU Tian. Establishment of Regeneration in Vitro System of Camellia japonica and Relationship Between Endogenous Hormones and Adventitious Bud Differentiation [J]. Acta Horticulturae Sinica, 2024, 51(8): 1891-1905.
[4]	LI Yadi, WANG Hanxiang, HU Baigeng, YANG Hui, HU Xinxi, XIONG Xingyao, WANG Wanxing. Research Progress on Plant Growth Promoting Rhizobacteria to Alleviate Abiotic Stress Tolerance of Horticultural Crops [J]. Acta Horticulturae Sinica, 2024, 51(8): 1964-1976.
[5]	LI Yachen, ZHENG Yanmei, SONG Wenpei, LI Dawei, LIANG Hong, and ZHANG Xianzhi, . Research Progress on the Role of Hydrogen-Rich Water in Plant Growth and Development and Stress Response [J]. Acta Horticulturae Sinica, 2024, 51(7): 1489-1500.
[6]	YANG Binan, LI Bowen, YANG Zhenyu, XU Yipeng, YAN Yunqing, LOU Yuxia, FENG Shucheng, MING Feng. The Mechanism of Heat Stress Response and the Exploration of Functional Genes in Rosa chinensis‘Angela’ [J]. Acta Horticulturae Sinica, 2024, 51(6): 1284-1296.
[7]	WU Xiangqi, SUN Li, YU Zheping, YU Qinpei, LIANG Senmiao, ZHENG Xiliang, QI Xingjiang, ZHANG Shuwen. Functional Study of MrSPL4 Gene in Response to Drought and Low Temperature Stress in Chinese Bayberry [J]. Acta Horticulturae Sinica, 2024, 51(5): 927-938.
[8]	LUO Jiandong, QIU Mengqing, ZHOU Huimin, XIE Weiwei, Huang Haixin, LIU Jieqi, ZHANG Zimin, XU Jian, CHEN Chengjie, HE Yehua, LIU Chaoyang. Cloning and Functional Analysis of Pineapple AcZFP1 Gene Under Low Temperature Stress [J]. Acta Horticulturae Sinica, 2024, 51(3): 495-508.
[9]	LIU Jinhong, WANG Zheng, YU Hao, XIN Yirui, QI Guoning, LIU Shenkui, REN Huimin. Identification of SLAC Gene Family in Phyllostachys edulis and Characterization of PheSLAC1 [J]. Acta Horticulturae Sinica, 2024, 51(3): 545-559.
[10]	ZHANG Wenhao, ZHANG Hui, LIU Yuting, WANG Yan, ZHANG Yingying, WANG Xinman, WANG Quanhua, ZHU Weimin, YANG Xuedong. Preliminary Transcriptome Analysis in Two Tomato Fruits Materials with Different Sugar Content [J]. Acta Horticulturae Sinica, 2024, 51(2): 281-294.
[11]	ZHAO Xiaobing, SUN Keyi, FAN Junmiao, DING Jing. Visible Fluorescence Analysis of Cytokinin and Auxin Responses in Tomato [J]. Acta Horticulturae Sinica, 2024, 51(2): 372-384.
[12]	ZHANG Yu, MEN Guotao, ZHAO Ju, LAN Kailong, ZHANG Ziyi. A New Cultivar of Jerusalem Artichoke‘Mengyu 3’ [J]. Acta Horticulturae Sinica, 2024, 51(2): 457-458.
[13]	LUO Xinrui, ZHANG Xiaoxu, WANG Yuping, WANG Zhi, MA Yuanyuan, ZHOU Bingyue. Identification and Expression Analysis of Trihelix Gene Family in Common Bean [J]. Acta Horticulturae Sinica, 2024, 51(12): 2775-2790.
[14]	FENG Zhijuan, LIU Na, ZHANG Guwen, BU Yuanpeng, WANG Bin, GONG Yaming. Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA [J]. Acta Horticulturae Sinica, 2024, 51(12): 2791-2799.
[15]	BU Wenxuan, YAO Yiping, HUANG Yu, YANG Xingyu, LUO Xiaoning, ZHANG Minhuan, LEI Weiqun, WANG Zheng, TIAN Jianing, CHEN Lujie, QIN Liping. Transcriptome Analysis of the Response of Tree Peony‘Hu Hong’Under High Temperature Stress [J]. Acta Horticulturae Sinica, 2024, 51(12): 2800-2816.