Analysis of the Difference of Flavonoids in Dendropanax dentiger Leaves with Different Drying Processes Based on Untargeted Metabolomics

doi:10.16420/j.issn.0513-353x.2023-0219

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 1069-1082.doi: 10.16420/j.issn.0513-353x.2023-0219

Previous Articles Next Articles

Analysis of the Difference of Flavonoids in Dendropanax dentiger Leaves with Different Drying Processes Based on Untargeted Metabolomics

YAO Fengping¹, WANG Yanbin², QIN Yuchuan², WANG Liling²^,^*()

¹ Qingyuan Forest Farm，Qingyuan，Zhejiang 323800，China
² Zhejiang Academy of Forestry Science，Hangzhou 310023，China

Received:2023-06-11 Revised:2023-09-19 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

Ultrahigh performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）was used to detect the flavonoids accumulated in leaves of Dendropanax dentiger under three drying methods（freeze-drying，hot-air drying and microwave drying）. Principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）were applied to identify the differential metabolites. The results indicated that 204 flavonoid compounds were identified，dominated by 53 flavonoids，37 flavonols and 24 isoflavones. A total of 80 differential compounds were screened，among which 69 different metabolites were found between hot-air drying and freeze-drying，67 were found between hot-air drying and microwave drying，and 45 were found between microwave drying and freeze-drying. The metabolites of these differences were mainly involved in the biosynthesis of secondary metabolites. The contribution rate of different substances to the characterization of different drying methods was obvious. Through the study of up-regulation and down-regulation of differential metabolites， it was found that the flavonoid components in the leaves could be retained better by freeze-drying，followed by microwave drying.

Key words: Dendropanax dentiger, untargeted metabolome, liquid chromatography-mass spectrometry, flavone

YAO Fengping, WANG Yanbin, QIN Yuchuan, WANG Liling. Analysis of the Difference of Flavonoids in Dendropanax dentiger Leaves with Different Drying Processes Based on Untargeted Metabolomics[J]. Acta Horticulturae Sinica, 2024, 51(5): 1069-1082.

Figures/Tables 8

Fig. 1 Total ion overlap diagram（a）and total ion flow diagram（b）

Fig. 2 Heat map of hot-air drying（HG），vacuum freeze-drying（DG），microwave drying（WB）groups to Dendropanax dentiger leaves

Fig. 3 PCA score plot of UPLC-MS/MS data from hot-air drying（HG），vacuum freeze-drying（DG），microwave drying（WB）to Dendropanax dentiger leaves（DG）

Fig. 4 OPLS-DA score plot of UPLC-MS/MS data from hot-air drying（HG），vacuum freeze-drying（DG），microwave drying（WB）to Dendropanax dentiger leaves

Fig. 5 Analysis of differential metabolites of Dendropanax dentiger leaves samples of hot-air drying（HG），vacuum freeze-drying（DG）and microwave drying（WB） Green dots are down-regulated metabolites，red dots are up-regulated metabolites，and black are metabolites with insignificant differences.

Fig. 6 Contents of flavonoids in the leaves of Dendropanax dentiger under different drying processes

Fig. 7 Violin plot of differential metabolites based on raw values of hot-air drying（HG）vac，uum freeze-drying（DG）and microwave drying（WB）to Dendropanax dentiger leaves The box in the middle indicates the interquartile range，the thin black line represents the 95% confidence interval，the black horizontal line in the middle is the median，and the outer shape indicates the density of the data distribution.

Fig. 8 KEGG pathway enrichment analysis of differential metabolites in Dendropanax dentiger leaves by hot-air drying（HG），vacuum freeze-drying（DG）and microwave drying（WB）

References 36

[1]	Anandhi R, Thomas P A, Geraldine P. 2014. Evaluation of the anti-atherogenic potential of chrysin in Wistar rats. Mol Cell Biochem, 385:103. doi: 10.1007/s11010-013-1819-z pmid: 24065391
[2]	Bassey E J, Cheng J H, Sun D W. 2021a. Improving drying kinetics,physicochemical properties and bioactive compounds of red dragon fruit (Hylocereus species)by novel infrared drying. Food Chemistry, 375:131886.
[3]	Bassey E J, Cheng J H, Sun D W. 2021b. Novel nonthermal and thermal pretreatments for enhancing drying performance and improving quality of fruits and vegetables. Trends in Food Science & Technology, 112:137-148.
[4]	Chen Yunqi, Lu Zhiming, Yang Yi, Wang Qiang. 2017. Active compounds extracted from leaves of Dendropanax dentiger and study on boost immunityfor rexbabbits. Non-timber Forst Products,(1):47-50. (in Chinese)
	陈云奇, 陆志敏, 杨丽, 王强. 2017. 树参嫩叶活性成分提取以及对提高獭兔免疫力的试验研究. 林副产品,(1):47-50.
[5]	Cruz L G, Guadarrama S V. 2016. Postharvest quality,soluble phenols,betalains content,and antioxidant activity of Stenocereuspruinosus and Stenocereusstellatus fruit. Postharvest Biol Technol, 111:69-76.
[6]	Deng L Z, Pan Z L, Mujumdar A S, Zhao J H, Zheng Z A, Gao Z J, Xiao H W. 2019. High-Humidity hot air impingement blanching(HHAIB)enhances drying quality of apricots by inactivating the enzymes,reducing drying time and altering cellular structure. Food Control, 96:104-111.
[7]	Fan Zhongxian. 2008. Health special dish-Tree ginseng. Rural Practical Science and Technology Information,(2):19-20. (in Chinese)
	范仲先. 2008. 保健特菜——树参菜. 农村实用科技信息,(2):19-20.
[8]	Geng Z, Wang J, Zhu L, Yu X L, Zhang Q, Li M Q, Hu B, Yang X H. 2023. Metabolomics provide a novel interpretation of the changes in flavonoids during sea buckthorn(Hippophaerhamnoides L.)drying. Food Chemistry, 413:135598.
[9]	Ghafoor K, Aljuhaimi F, Ozcan M M, Uslu N, Babiker E E, Ahmed I A M. 2020. Total phenolics,total carotenoids,individual phenolics and antioxidant activity of ginger(Zingiberofficinale)rhizome as affected by drying methods. LWT-Food Science and Technology, 126:109354.
[10]	Guo C, Zhang N, Liu C, Xue J J, Chu J Z, Yao X Q. 2020. Qualities and antioxidant activities of lotus leaf affected by different drying methods. Acta Physiologiae Plantarum, 42:14.
[11]	Huang Jingyao, Liu Chunmei, Qi Piliu, Liu Kangming. 1989. Studies on the antiarrhythmic effects of leaves of Dendropanax chevalieri(Vig.)Merr.et Chun. China J Chin Mater Med, 14 (6):367-370,384. (in Chinese)
	黄敬耀, 刘春梅, 齐丕骝, 刘康民. 1989. 树参叶抗心律失常作用的研究. 中国中药杂志, 14 (6):367-370,384.
[12]	Hou Rui, Chen Qi, Wang Li, Jia Yuchen, Li Shaowei. 2015. The research progress of anthocyanins and it’s biological activities. Progress in Modern Biomedicine, 15 (28):5590. (in Chinese)
	侯锐, 陈琦, 王利, 贾宇臣, 李少伟. 2015. 花青素及其生物活性的研究进展. 现代生物医学进展, 15 (28):5590.
[13]	Jing N, Wang M, Gao M, Zhong Z X, Ma Y P, Wei A Z. 2021. Color sensory characteristics,nutritional components and antioxidant capacity of Zanthoxylumbungeanum Maxim. as affected by different drying methods. Industrial Crops & Products, 160:113167.
[14]	Jiang Xiang, Wei Huan, Huang Yanhong, Lü Min, Huang Haixia, Wei Chunmeng, Yuan Rongsui. 2020. Determination of 14 plant growth regulators in Ophiopogon Japonicas,Chinese yam and chrysanthemum by UPLC-MS/MS. China Pharmacist, 23 (2):264-269. (in Chinese)
	蒋湘, 韦环, 黄燕红, 吕敏, 黄海霞, 韦春梦, 袁榕穗. 2020. 超高效液相色谱—串联质谱法测定麦冬、山药和菊花中14种植物生长调节剂. 中国药师, 23 (2):264-269.
[15]	Lauxmann M, Borsani J. 2014. Deciphering the metabolic pathways influencing heat and cold responses during post-harvest physiology of peach fruit. Plant Cell Environ, 37:601-616.
[16]	Ledesma-escobar C A, Priego-capote F, Luque de castro M D. 2016. Comparative study of the effect of sample pretreatment and extraction on the determination of flavonoids from lemon(Citrus limon). PLoS ONE, 11:e0148056.
[17]	Liu J, Jiao Z, Zhang C, Yang W B, Liu H, Lv Z Z. 2018. Effects of different drying methods on phenolic contents,antioxidant,and tyrosinase inhibitory activity of peach blossoms. J Food Meas Charact, 12:2339-2348.
[18]	Liu Y H, Miao S A, Wu J Y. 2015. Drying characteristics and modeling of vacuum far-infrared radiation drying of FlosLonicerae. J Food Proc Preserv, 39:338-348.
[19]	Liu Yuyao, Liao Fujuan, Li Yushan, Chen Hongping, Wang Fu, Hu Yuan, Liu Youping. 2023. Effects of different drying methods on quality of male flowers of Eucommia ulmoides based on color and chemical composition. Journal of Chinese Traditional Medicine, 48 (7):1876-1884. (in Chinese)
	刘宇瑶, 廖富娟, 李雨珊, 陈鸿平, 王福, 胡媛, 刘友平. 2023. 基于颜色和化学成分研究不同干燥方法对杜仲雄花品质影响. 中国中药杂志, 48 (7):1876-1884.
[20]	Lu Meng, Su Gang, Cheng Ju, Chen Jianjun, Zhou Tao, Zhao Junli, Wang Jing, Zhang Shasha, Ma Qinglong, Xie Xiaodong. 2023. Research progress on anti-tumor activity and mechanism of flavonoids. West China Journal of Pharmaceutical Sciences, 38 (2):236-240. (in Chinese)
	路梦, 苏刚, 程菊, 陈建军, 周涛, 赵君利, 王静, 张莎莎, 马青龙, 谢小冬. 2023. 黄酮类化合物抗肿瘤活性及机制的研究进展. 华西药学杂志, 38 (2):236-240.
[21]	Mei Guixue, Sun Jie, Yin Shujun, Wang Xiaojing. 2018. Research progress on flavonoids for prevention and treatment of diabetes mellitus and its complications. Food and Drugs, 20 (4):307. (in Chinese)
	梅桂雪, 孙捷, 殷姝君, 王晓静. 2018. 黄酮类化合物防治糖尿病及其并发症的研究进展. 食品与药品, 20 (4):307.
[22]	Nie H, Chen H C, Li G L, Su K Z, Song M B, Duan Z H, Li X C, Cao X H, Huang J G, Huang S Q, Luo Y H. 2021. Comparison of flavonoids and phenylpropanoids compounds in Chinese water chestnut processed with different methods. Food Chemistry, 335:127662.
[23]	Onwude D I, Hashim N, Abdan K, Janius R, Chen G N. 2019. The effectiveness of combined infrared and hot-air drying strategies for sweet potato. Journal of Food Engineering, 241:75-87.
[24]	Park S E, Sapkota K, Choi J H, Kim M K, Kim Y H, Kim K M, Kim K. 2014. Rutin from Dendropanax morbifera Léveille protects humandopaminergic cells against rotenone induced cell injurythrough inhibiting JNK and p 38 MAPK signaling. Neurochem Res, 39 (4):707-718.
[25]	Qian Hua, He Zhihua, Shao Shunliu, Yang Zaijuan, Hong Lixing. 2003. Resources and evaluation of edible wild plants in Zhejiang. Journal of Zhejiang Forestry Science and Technolog, 23 (4):36-42. (in Chinese)
	钱华, 何志华, 邵顺流, 杨在娟, 洪利兴. 2003. 浙江森林野菜资源及其评价. 浙江林业科技, 23 (4):36-42.
[26]	Qiu Y, Bi J F, Jin X, Wu X Y, Hu L N, Chen L M. 2022. Investigation on the rehydration mechanism of freeze-dried and hot-air dried shiitake mushrooms from pores and cell wall fibrous material. Food Chemistry, 383:132360.
[27]	Su Xuemei, Lai Lincheng, Yin Shuhua, Yao Bei, Gong Xiaoyu, Chen Yuyu. 2021. Comparative analysis of gum traditional chinese medicine based on UPLC-MS and metabonomics. China Pharmacist, 24 (6):1025-1029. (in Chinese)
	苏雪媚, 赖林城, 尹淑华, 姚贝, 巩晓宇, 陈雨钰. 2021. 基于UPLC-MS技术和代谢组学对胶类中药的比较分析. 中国药师, 24 (6):1025-1029.
[28]	Wang A M, Li R S, Ren L, Gao X L, Zhang Y G, Ma Z M, Ma D F, Luo Y H. 2018a. A comparative metabolomics study of flavonoids in sweet potato with different flesh colors[Ipomoea batatas(L.)Lam]. Food Chemistry, 260:124-134.
[29]	Wang J, Yang X H, Mujumdar A S, Fang X M, Zhang Q, Zheng Z A, Gao Z J, Xiao H W. 2018b. Effects of high-humidity hot air impingement blanching(HHAIB)pretreatment on the change of antioxidant capacity,the degradation kinetics of red pigment,ascorbic acid in dehydrated red peppers during storage. Food Chemistry, 259:65-72.
[30]	Yuan J, Hao L J, Wu G, Wang S, Duan J A, Xie G Y, Qin M J. 2015. Effects of drying methods on the phytochemicals contents and antioxidant properties of chrysanthemum flower heads harvested at two developmental stages. J Funct Foods, 19:786-795.
[31]	Yue X H, Abdallah A M, Xu Z M. 2010. Thermal dynamic properties of isoflavones during dry heating. International Journal of Food Science & Technology, 45 (9):1755-1963.
[32]	Yu Xiangying, Zheng Jianan, Chen Xiaochun, Cai Zhaochu, Li Lin. 2022. Effects of drying methods on the content of 7 kinds of flavonoids and antioxidant activity in Citri retriculatae Pericarpium. Journal of Food Safety and Quality, 13 (7):2075-2083. (in Chinese)
	余祥英, 郑佳楠, 陈晓纯, 蔡昭楚, 李琳. 2022. 不同干燥方式对陈皮中7种黄酮含量及抗氧化性的影响. 食品安全质量检测学报, 13 (7):2075-2083
[33]	Zhang L, Liu T F, Xue Y J, Liu C, Ru H, Dong M H, Yu Z F. 2016. Effects of drying methods on the aroma components and quality of Capsella Bursa-Pastoris L. J Food Process Eng, 39:107-120.
[34]	Zheng L P, He Z G, Wu Z J, Zhang C A. 2012. Chemical constituents from Dendropanax dentiger. Chem Nat Compd, 48 (5):883-885.
[35]	Zheng L P, He Z G. 2013. Antioxidant activity of phenolic compounds from Dendropanax dentiger(Harms)Merr. Asian J Chem: 25 (14):7809-7812.
[36]	Zhong Yike, Wu Di, Hua Xiaodan, Zhao Tong, Wang Yongxia. 2019. Progress in the study of antibacterial effect of flavonoids compounds. China Food Additives, 30 (8):166. (in Chinese)
	钟宜科, 吴迪, 花晓丹, 赵彤, 王永霞. 2019. 黄酮类化合物抑菌作用研究进展. 中国食品添加剂, 30 (8):166.

Analysis of the Difference of Flavonoids in Dendropanax dentiger Leaves with Different Drying Processes Based on Untargeted Metabolomics

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 36

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	WU Zhuoqun, CHEN Pengxu, CHEN Siyi, ZHANG Fuqiong, FANG Hong, ZHU Jian, XIE Zongzhou, LI Chunlong, and LIU Jihong, . Differences in Occurrence of Fruit Sunburn Between Satsuma Mandarin and Ponkan Tangerine and Possible Mechanisms [J]. Acta Horticulturae Sinica, 2024, 51(12): 2743-2757.
[2]	TIAN Jie, ZHOU Qianyi, TIE Yuanyu, SUN Haihong, and HUANG Sijie. Metabolomic Analysis of Garlic Seedling Under Drought Stress [J]. Acta Horticulturae Sinica, 2024, 51(1): 133-144.
[3]	Cheng Shuiyuan;Wang Yan;Li Junkai;Gu Manru;and Shu Huairui. Studies on the Change of Flavone Contents and Its Distribution in Ginkgo biloba Leaves [J]. ACTA HORTICULTURAE SINICA, 2001, 28(4): 353-355.