Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

doi:10.16420/j.issn.0513-353x.2024-0626

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 857-871.doi: 10.16420/j.issn.0513-353x.2024-0626

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

YUAN Juanwei¹^,², JIA Li², WANG Han², YAN Congsheng², ZHANG Qi’an², YU Feifei², GAN Defang¹^,^*(), JIANG Haikun²^,^*()

¹ College of Horticulture，Anhui Agricultural University，Hefei 230036，China
² Key Laboratory of Germplasm Creation and Utilisation of Horticultural Crops，Co-construction Ministry of Agriculture and Rural Affairs and Anhui Province，Key Laboratory of Genetic Improvement and Hingh-Efficiency Cultivation of Horticultural Crop，Institute of vegetable，Anhui Academy of Agricultural Sciences，Hefei 230031，China

Received:2024-12-24 Revised:2025-03-11 Online:2025-05-08 Published:2025-04-25
Contact: GAN Defang, JIANG Haikun

Abstract

Abstract:

In order to screen pepper germplasm for disease resistance and explore disease resistance related genes，12 pairs of SSR primers for disease resistance were used for initial and secondary screening of 214 pepper germplasm materials，resulting in 6 pairs of SSR resistant primers with good polymorphism and 56 pepper germplasm with disease resistance. Inoculate resistant pepper germplasm with pepper phytophthora spore suspension and record the disease status，among which 11 were highly resistant. Transcriptome sequencing was performed on pepper stems at different stages after inoculation，and GO，KEGG enrichment，and WGCNA analysis were performed on differentially expressed genes. A total of 15 candidate genes related to disease resistance were screened.

Key words: pepper, Phytophthora capsici, SSR molecular marker, transcriptome, WGCNA, candidate gene

YUAN Juanwei, JIA Li, WANG Han, YAN Congsheng, ZHANG Qi’an, YU Feifei, GAN Defang, JIANG Haikun. Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources[J]. Acta Horticulturae Sinica, 2025, 52(4): 857-871.

Figures/Tables 12

References 38

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引物名称Primer name	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
L0101	AACCAGCAATCCCATGAAAACC	GGGCTTTGGGGAGAATAGTGTG
L0132	ATTTTCCTCCATGCCTTCCTATCA	GTTTAGAAGCCCAGGGAATACTCGTACC
L0311	TGCTGGGAAAGATCTCAAAAGG	ATCAAGGAAGCAAACCAATGC
L0315	CACTGCAACTTGTGTACTACTACCAC	CGCTCTTACTGTTTCAAACTTATCTC
L0338	ACCAACTCTCTCTCGTGTCAACAA	GTTTAGGGTACCTTTTGGTGAAGGC
L0501	CCCGCGAAATCAAGGTAAT	AAAGCTATTGCTACTGGGTTCG
L0503	ATGGAGCCATCTGACAGCAAATTC	GTTTCCGAGCTCGATGAGGATGAACTA
L0505	ATGCAGAAGCCTCATCAATTTGGG	GTTTGTCATCATGATCCTCGTTTTTGG
L0601	AGTGTTGCACGTGTCGAGAAACTA	GTTTAGCCTTCAACGTTGGTTTAGCA
L0710	ATATTGTCACGTGGCTTCTCAACA	GTTTCAATAGATTAGTCATCCGTTGCCA
L0805	ACTTTCCTGCAAAGAGCGTATGAT	GTTTGACCTTTCCTCTTATTTCGTGGGG
OpD04.717	CCATAAGGGTTGGTAAATTTACAAAG	TCGAGAGATAATTCAGATAGTATAATC

引物名称Primer name	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
L0101	AACCAGCAATCCCATGAAAACC	GGGCTTTGGGGAGAATAGTGTG
L0132	ATTTTCCTCCATGCCTTCCTATCA	GTTTAGAAGCCCAGGGAATACTCGTACC
L0311	TGCTGGGAAAGATCTCAAAAGG	ATCAAGGAAGCAAACCAATGC
L0315	CACTGCAACTTGTGTACTACTACCAC	CGCTCTTACTGTTTCAAACTTATCTC
L0338	ACCAACTCTCTCTCGTGTCAACAA	GTTTAGGGTACCTTTTGGTGAAGGC
L0501	CCCGCGAAATCAAGGTAAT	AAAGCTATTGCTACTGGGTTCG
L0503	ATGGAGCCATCTGACAGCAAATTC	GTTTCCGAGCTCGATGAGGATGAACTA
L0505	ATGCAGAAGCCTCATCAATTTGGG	GTTTGTCATCATGATCCTCGTTTTTGG
L0601	AGTGTTGCACGTGTCGAGAAACTA	GTTTAGCCTTCAACGTTGGTTTAGCA
L0710	ATATTGTCACGTGGCTTCTCAACA	GTTTCAATAGATTAGTCATCCGTTGCCA
L0805	ACTTTCCTGCAAAGAGCGTATGAT	GTTTGACCTTTCCTCTTATTTCGTGGGG
OpD04.717	CCATAAGGGTTGGTAAATTTACAAAG	TCGAGAGATAATTCAGATAGTATAATC

基因Gene	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
Can02G020380	CAAGAGATGGTATTATACGAAGT	TGTGATGGACGAAGGATAT
Can05G006970	TATTCTACTGATAATGTCCA	AGTCTCACTAATGTTGTT
Can05G009560	GCGATGAATGGAAATAAC	TTAGGCACAACAATAGTC
Can08G006950	TAGACGATGGCTATAAGT	TTATCACATACCGAGAGT
Can07G001460	GGCAAGTTCAAGAAGACA	CAAGCATTAACAGCATCAC
Can12G034470	TTAGTTACAAGCACTTCTA	GCCACATCAATTATTAGC
Can09G002600	ACTTCTTCTGTTGCTGAT	TTGTGGATGTTGTTGTTG
Can05G018720	ATGAAGTCGTCTGTAGATTA	CCAATTCCACAACTCCTA
NewGene_12037	AGTGGAGGTCTAATAGTG	TGTATCAGAGCAAGGTTA
Can01G018740	CAACACTACAAGCAACAT	TAGCATAACGATTCACTATC
β-actin	AATCAATCCCTCCACCTCTTCACTC	CATCACCAGCAAATCCAGCCTT

基因Gene	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
Can02G020380	CAAGAGATGGTATTATACGAAGT	TGTGATGGACGAAGGATAT
Can05G006970	TATTCTACTGATAATGTCCA	AGTCTCACTAATGTTGTT
Can05G009560	GCGATGAATGGAAATAAC	TTAGGCACAACAATAGTC
Can08G006950	TAGACGATGGCTATAAGT	TTATCACATACCGAGAGT
Can07G001460	GGCAAGTTCAAGAAGACA	CAAGCATTAACAGCATCAC
Can12G034470	TTAGTTACAAGCACTTCTA	GCCACATCAATTATTAGC
Can09G002600	ACTTCTTCTGTTGCTGAT	TTGTGGATGTTGTTGTTG
Can05G018720	ATGAAGTCGTCTGTAGATTA	CCAATTCCACAACTCCTA
NewGene_12037	AGTGGAGGTCTAATAGTG	TGTATCAGAGCAAGGTTA
Can01G018740	CAACACTACAAGCAACAT	TAGCATAACGATTCACTATC
β-actin	AATCAATCCCTCCACCTCTTCACTC	CATCACCAGCAAATCCAGCCTT

基因Gene	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
NewGene_12193	GACCACATACCACTCAAG	TCCACAATCCACAATCAG
Can07G034320	TTGATGTAGACGAAGTGA	CATCTCCTGTAAGCATTG
Can01G023360	TGCTATACAATCTTCAAT	CTATTCAATCTCATCCAT
Can07G002780	ATGACAGACAAGGTAGAT	TTACGGTTAAGAGGAGAA
Can04G014490	GATATGTTGCTTGATGGTGGTGAT	GCTTATCTCTTGCTGCGTGAA
Can04G029410	ATGCTAACTTGGTAACAC	TTCATATTGGTCCTTCTTG
NewGene_13172	ATCCTTACAACAACAACA	TTAGAGGTAGAGGTATGG
Can09G002790	GCACTGGTATTGAAGAAG	AAGATTGAGGTATTGAAGATG
Can10G030550	ATAACATTCCATTCTCCAA	CCTCTTCTTCATCATCAG
Can07G025330	GAGTTCCAGTTGATATGA	GCTGTATCCATTATCTCTA
NewGene_11156	AAGGCTCATTCTATTATCAT	AAGGTAGGTATCTCAAGT
Can06G035780	TCCTTACGAGAACAATCATC	GCAAGAGTCACATTATCCA
Can09G017290	GACAACACACCCTATACT	ACATATCTGGATTCTCTTCA
Can06G036020	CATCTTCTACTCATCCACTTA	ATTCTTGCTCAATGTTCAC
Can08G028150	GGTAATTGGAGAGGACAA	AGACATCAGTTGGAAGTT
β-actin	AATCAATCCCTCCACCTCTTCACTC	CATCACCAGCAAATCCAGCCTT

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

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品种 Cultivar	果形 Fruit shape	病情指数 Disease index	抗性评价 Resistance evaluation	发病率/% Morbidity
H14-205	长牛角形Long bovine horn shape	4.44	R	11.00
V06C1825	长指形Long finger shape	0	HR	0
H18-GPJ	长牛角形Long bovine horn shape	6.67	R	22.00
XXYSJ-1	扁灯笼形Flat lantern shape	0	HR	0
小米辣	短指形Short finger shape	0	HR	0
H19-STJ-8	长锥形Long conical shape	4.00	R	15.00
H19-SZL-75	短羊角形Short caprine horn shape	19.99	MR	46.00
V06C0954	短羊角形Short caprine horn shape	2.00	R	10.00
佘干椒	短羊角形Short caprine horn shape	5.00	R	25.00
V06C0132	短羊角形Short caprine horn shape	1.18	R	6.00
CF-4	长羊角形Long caprine horn shape	1.11	R	6.00
H19-STJ-74	短指形Short finger shape	0	HR	0
H17-XM39-01	长锥形Long conical shape	3.53	R	18.00
H18-PC-YS-02	长羊角形Long caprine horn shape	1.43	R	7.00
V06C0670	长锥形Long conical shape	4.00	R	15.00
V06C1220	短羊角形Short caprine horn shape	4.00	R	20.00
8701	短牛角形Short bovine horn shape	10.00	R	25.00
V06C0503	长羊角形Long caprine horn shape	4.00	R	5.00
H19-1021	长牛角形Long bovine horn shape	10.00	R	15.00
H19-STJ-1	短牛角形Short bovine horn shape	14.00	MR	25.00
DS701	圆灯笼形Round lantern shape	1.00	HR	5.00
V06C0573	短羊角形Short caprine horn shape	18.00	MR	45.00
V06C172	短羊角形Short caprine horn shape	0	HR	0
AHYYH005394	短指形Short finger shape	6.00	R	15.00
H11-003	长灯笼形Long lantern shape	2.00	R	5.00
H12-115	长羊角形Long caprine horn shape	15.00	MR	35.00
H14-45	长指形Long finger shape	0	HR	0
AHYYH004309	长羊角形Long caprine horn shape	12.00	MR	30.00
SZHHFB01	线形Line shape	0	HR	0
H15-066	长羊角形Long caprine horn shape	0	HR	0
潜山辣椒	短羊角形Short caprine horn shape	6.00	R	15.00
H14-070	短羊角形Short caprine horn shape	3.00	R	10.00
AHYYH004115	长指形Long finger shape	4.00	R	10.00
H19-STJ-76	短羊角形Short caprine horn shape	17.00	MR	35.00
H11-011	短牛角形Short bovine horn shape	6.00	R	15.00
H19-STJ-10	短羊角形Short caprine horn shape	18.00	MR	25.00
V06C1902	长羊角形Long caprine horn shape	6.15	R	23.00
V06C1704	短羊角形Short caprine horn shape	4.00	R	15.00
H19-SZL-25	长牛角形Long bovine horn shape	11.00	MR	20.00
H19-LSF	长羊角形Long caprine horn shape	18.67	MR	40.00
M19-SZL-41	线形Line shape	7.00	R	25.00
AHYYH005152	短指形Short finger shape	3.16	R	11.00
Y-417	线形Line shape	7.37	R	16.00
2016-56	长灯笼形Long lantern shape	16.67	MR	25.00
V06C1657	长灯笼形Long lantern shape	6.15	R	31.00
H18-56-3	线形Line shape	14.29	MR	38.00
H18×9931 BS1	短羊角形Short caprine horn shape	4.00	R	5.00
V06C1205	短羊角形Short caprine horn shape	16.92	MR	31.00
H19-STJ-3	长指形Long finger shape	3.00	R	15.00
H19-SZL-70	长指形Long finger shape	5.00	R	15.00
V06C0330	长羊角形Long caprine horn shape	10.43	MR	22.00
V06V0960	长指形Long finger shape	0	HR	0
AHYYH005208	长羊角形Long caprine horn shape	6.00	R	15.00
AHYYH004364	短羊角形Short caprine horn shape	2.00	R	10.00
H19-STJ-7	短指形Short finger shape	1.00	HR	5.00
AHYYH005174	长指形Long finger shape	2.86	R	7.00

通路 Pathway	基因ID Gene ID	log₂FC	基因特征 Genetic trait
MAPK信号通路，植物—病原互作，激素信号转导 MAPK signaling pathway，plant pathogen interaction，plant hormone signaling transduction	NewGene_12193	5.13	BAK1
	Can07G034320	2.01	BAK1
	Can01G023360	6.06	BAK1
	Can08G028150	9.78	PR1
MAPK信号通路，植物—病原互作 MAPK signaling pathway，plant pathogen interaction	Can07G002780	7.42	FLS2
	Can04G014490	3.36	WRKY33
	NewGene_13172	1.75	FLS2
	Can04G029410	1.46	FLS2
植物—病原互作 Plant pathogen interaction	Can09G002790	2.65	RPS2
	Can10G030550	12.22	RPS2
	Can07G025330	2.91	PBS1
	NewGene_11156	3.21	RPM1
	Can06G035780	5.60	R1B-23
	Can09G017290	2.79	RIN_4
	Can06G036020	2.49	R1A-4

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[3]	ZOU Fen, NIE Wuxue, XIANG Qing, HE Liegan, WANG Chunqing, YU Junhui, HUANG Xiaomei, HUANG Shuilin, MA Huigang. A New Pod Pepper Cultivar‘Yuehong 461’ [J]. Acta Horticulturae Sinica, 2025, 52(S1): 119-120.
[4]	LU Jingqi, LIU Feng, ZOU Xuexiao. A New Pepper Cultivar‘Runjianghong 17’ [J]. Acta Horticulturae Sinica, 2025, 52(S1): 121-122.
[5]	WANG Guanghua, LI Lei, LIU Ling. A New Processing Pepper Cultivar‘Hongbaota’ [J]. Acta Horticulturae Sinica, 2025, 52(S1): 123-124.
[6]	TANG Lingli, HE Yuhua, WANG Qingtao, AN Lulu, XU Yongyang, ZHANG Jian, KONG Weihu, HU Keyun, ZHAO Guangwei. Hormonal and Transcriptome Analysis of Ripe Fruits of Non-Climacteric and Climacteric Melon [J]. Acta Horticulturae Sinica, 2025, 52(4): 883-896.
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