Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora
capsici Resistance in Pepper Germplasm Resources

doi:10.16420/j.issn.0513-353x.2024-0626

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 857-871.doi: 10.16420/j.issn.0513-353x.2024-0626

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

YUAN Juanwei^1,2，JIA Li²，WANG Han²，YAN Congsheng²，ZHANG Qi’an²，YU Feifei²，GAN Defang^1,*(gandf@ahau.edu.cn)，and JIANG Haikun^2,*(jhk211@163.com)

¹College of Horticulture，Anhui Agricultural University，Hefei 230036，China ²Key Laboratory of Germplasm Creation and Utilisation of Horticultural Crops，Co-construction Ministry of Agriculture and Rural Affairs and Anhui Province，Key Laboratory of Genetic Improvement and Hingh-Efficiency Cultivation of Horticultural Crop，Institute of vegetable，Anhui Academy of Agricultural Sciences，Hefei 230031，China

Received:2024-12-24 Revised:2025-03-11 Online:2025-04-25 Published:2025-04-25
Contact: GAN Defang，JIANG Haikun

Abstract

Abstract: In order to screen pepper germplasm for disease resistance and explore disease resistance related genes，12 pairs of SSR primers for disease resistance were used for initial and secondary screening of 214 pepper germplasm materials，resulting in 6 pairs of SSR resistant primers with good polymorphism and 56 pepper germplasm with disease resistance. Inoculate resistant pepper germplasm with pepper phytophthora spore suspension and record the disease status，among which 11 were highly resistant. Transcriptome sequencing was performed on pepper stems at different stages after inoculation，and GO，KEGG enrichment，and WGCNA analysis were performed on differentially expressed genes. A total of 15 candidate genes related to disease resistance were screened.

Key words: pepper, Phytophthora capsici, SSR molecular marker, transcriptome, WGCNA, candidate gene

CLC Number:

S 641.3

YUAN Juanwei, JIA Li, WANG Han, YAN Congsheng, ZHANG Qi’an, YU Feifei, GAN Defang, JIANG Haikun. Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources[J]. Acta Horticulturae Sinica, 2025, 52(4): 857-871.

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Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

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