Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (8): 1743-1757.doi: 10.16420/j.issn.0513-353x.2023-0593
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
KUANG Meimei1,2, LI Li2, MA Jianwei3, LIU Yuan3, JIANG Hongfei3, LEI Rui2, MAN Yuping2, WANG Yifan4, HUANG Bo3, WANG Yanchang2,*(), LIU Shibiao1,*(
)
Received:
2024-01-12
Revised:
2024-05-20
Online:
2024-08-25
Published:
2024-08-21
Contact:
WANG Yanchang, LIU Shibiao
KUANG Meimei, LI Li, MA Jianwei, LIU Yuan, JIANG Hongfei, LEI Rui, MAN Yuping, WANG Yifan, HUANG Bo, WANG Yanchang, LIU Shibiao. Screening of Kiwifruit Canker Resistance-Related Genes Based on the Transcriptome Sequencing Analysis of Hybrids from Actinidia chinensis[J]. Acta Horticulturae Sinica, 2024, 51(8): 1743-1757.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0593
基因编号 Gene ID | 引物序列 Primer sequence | 产物大小/bp Product length | 退火温度/℃ Tm |
---|---|---|---|
Actin | F:5′-TGAGAGATTCCGTTGCCCAGAAGT-3′/ R:5′-TTCCTTACTCATGCGGTCTGCGAT-3′ | 193 | 57.4/57.4 |
Acc23292 | F:5′-GGGAGCTGTCATTCGAGGAA-3′/ R:5′-GATAATCGCCCAGCCTTTCG-3′ | 140 | 53.8/55.0 |
Acc14142 | F:5′-TCAATCCACCATGCCAGAGT-3′/ R:5′-CAGCTGCCTTCGTGTTCTTT-3′ | 181 | 51.8/51.8 |
Acc28207 | F:5′-ATGGACCATCTACTTTGCTT-3′/ R:5′-GTGCTGAGTGCAATCTGCGG-3′ | 128 | 51.4/55.3 |
Acc04756 | F:5′-ATGTCGACGAGGAAGGATGT-3′/ R:5′-CGGATCGATCTTGCTAATGC-3′ | 126 | 56.5/58.3 |
Acc26787 | F:5′-CGTATGCTCTCCGAGTCACC-3′/ R:5′-ACTTGGCCGGAGAATTGGTT-3′ | 221 | 57.8/60.9 |
Acc04136 | F:5′-GACGAGTCGTGGGTTGTAGG-3′/ R:5′-TTTGGTCACTCCAGCTTCCG-3′ | 159 | 57.6/60.9 |
Acc06864 | F:5′-CTAGCCACGGTTCACTCCTC-3′/ R:5′-ATGTTCTCGCCGTACTTCCC-3′ | 203 | 55.9/53.8 |
Acc06865 | F:5′-AACACCGTTCGTGACCAAGT-3′/ R:5′-TGGTTAGCGTAGTTCTCGGC-3′ | 83 | 51.8/53.8 |
Acc06866 | F:5′-AACCAGAGGTCTGGCGATTG-3′/ R:5′-AACCTTACCTGAGGCACACG-3′ | 171 | 53.8/53.8 |
Acc06484 | F:5′-ATCACTCAGCCTGCCTCAAC-3′/ R:5′-CTTGGTCACGCAATACGCAG-3′ | 251 | 53.8/53.8 |
Table 1 Primers for qRT-PCR
基因编号 Gene ID | 引物序列 Primer sequence | 产物大小/bp Product length | 退火温度/℃ Tm |
---|---|---|---|
Actin | F:5′-TGAGAGATTCCGTTGCCCAGAAGT-3′/ R:5′-TTCCTTACTCATGCGGTCTGCGAT-3′ | 193 | 57.4/57.4 |
Acc23292 | F:5′-GGGAGCTGTCATTCGAGGAA-3′/ R:5′-GATAATCGCCCAGCCTTTCG-3′ | 140 | 53.8/55.0 |
Acc14142 | F:5′-TCAATCCACCATGCCAGAGT-3′/ R:5′-CAGCTGCCTTCGTGTTCTTT-3′ | 181 | 51.8/51.8 |
Acc28207 | F:5′-ATGGACCATCTACTTTGCTT-3′/ R:5′-GTGCTGAGTGCAATCTGCGG-3′ | 128 | 51.4/55.3 |
Acc04756 | F:5′-ATGTCGACGAGGAAGGATGT-3′/ R:5′-CGGATCGATCTTGCTAATGC-3′ | 126 | 56.5/58.3 |
Acc26787 | F:5′-CGTATGCTCTCCGAGTCACC-3′/ R:5′-ACTTGGCCGGAGAATTGGTT-3′ | 221 | 57.8/60.9 |
Acc04136 | F:5′-GACGAGTCGTGGGTTGTAGG-3′/ R:5′-TTTGGTCACTCCAGCTTCCG-3′ | 159 | 57.6/60.9 |
Acc06864 | F:5′-CTAGCCACGGTTCACTCCTC-3′/ R:5′-ATGTTCTCGCCGTACTTCCC-3′ | 203 | 55.9/53.8 |
Acc06865 | F:5′-AACACCGTTCGTGACCAAGT-3′/ R:5′-TGGTTAGCGTAGTTCTCGGC-3′ | 83 | 51.8/53.8 |
Acc06866 | F:5′-AACCAGAGGTCTGGCGATTG-3′/ R:5′-AACCTTACCTGAGGCACACG-3′ | 171 | 53.8/53.8 |
Acc06484 | F:5′-ATCACTCAGCCTGCCTCAAC-3′/ R:5′-CTTGGTCACGCAATACGCAG-3′ | 251 | 53.8/53.8 |
Fig. 1 Comparison of the phenotypes of disease-resistant individual‘21-2-4’and susceptible individual‘19-3-11’ isolated branches of kiwifruit hybrid progeny 35 d after inoculation with Psa
Fig. 2 GO and KEGG enrichment analysis of DEGs in kiwifruit disease resistant monocot‘21-2-4’at 6 h and 24 h after inoculation with Psa versus before inoculation
Fig. 3 GO and KEGG Enrichment analysis of DEGs between kiwifruit disease-resistant monocot‘21-2-4’ and susceptible monocot‘19-3-11’at 6 h and 24 h after inoculation with Psa
Fig. 4 Expression patterns of DEGs involved in plant-pathogen interaction pathway in‘21-2-4’and‘19-3-11’ A:Involved in the plant-pathogen interaction pathway;B:Involved in the ethylene/jasmonate signalling pathway;C:Involved in the salicylic acid signalling pathway;D:Involved in the phenylpropane biosynthesis pathway.
基因编号 Gene ID | 表达量 FPKM(‘21-2-4’) | KO | 途径 Pathway | NR注释 NR_annotation | ||
---|---|---|---|---|---|---|
0 h | 6 h | 24 h | ||||
Acc06866 | 33.27 b | 305.75 b | 2 308.34 a | PR1 | PTI、SA | 病程相关蛋白Pathogenesis-related protein |
Acc06865 | 64.83 b | 537.47 b | 2 678.93 a | PR1 | PTI、SA | 病程相关蛋白Pathogenesis-related protein |
Acc06864 | 367.59 b | 2 171.17 b | 10 865.11 a | PR1 | PTI、SA | 病程相关蛋白Pathogenesis-related protein |
Acc06484 | 14.20 b | 111.05 a | 25.56 b | FLS2 | PTI | LRR受体样激酶LRR receptor-like serine/threonine-protein kinase |
Acc25328 | 2.92 b | 15.19 a | 7.70 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
novel.6186 | 2.69 b | 12.26 a | 6.08 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
novel.36390 | 2.59 b | 11.14 a | 6.99 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
Acc25330 | 3.16 b | 12.70 a | 7.51 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
Acc23527 | 2.24 b | 7.74 a | 10.43 ab | CPK | PTI | 钙依赖蛋白激酶Calcium-dependent protein kinase |
Acc18178 | 11.18 b | 30.83 a | 39.39 a | EDS1 | ETI | EDS1样蛋白 Protein EDS1B like |
Acc07305 | 28.87 b | 86.32 a | 89.80 a | CTSF | ETI | 半胱氨酸蛋白酶Cysteine protease |
Acc11129 | 78.04 b | 159.43 a | 305.97 a | CTSF | ETI | 半胱氨酸蛋白酶Cysteine protease |
Acc02718 | 10.53 b | 32.00 a | 31.63 a | RAR1 | ETI | 富含半胱氨酸和组氨酸结构域蛋白 Cysteine and histidine-rich domain-containing protein |
Acc21162 | 321.06 ab | 193.38 b | 692.83 a | PR1 | SA | 病程相关蛋白基本形式Basic form of pathogenesis-related protein like |
Acc29710 | 2.74 b | 5.29 ab | 11.16 a | 4CL | PAL | 类4香豆酸辅酶 A 4-coumarate-CoA ligase-like |
Acc01331 | 7.41 b | 23.73 a | 12.13 ab | 4CL | PAL | 4香豆酸辅酶 A 4-coumarate-CoA ligase |
Acc16561 | 160.02 b | 284.77 ab | 449.28 a | POD | PAL | 过氧化物酶Peroxidase |
Acc20997 | 1.91 b | 1.70 b | 10.94 a | POD | PAL | 过氧化物酶Peroxidase |
Acc00538 | 5.41 b | 28.43 a | 37.94 a | POD | PAL | 过氧化物酶Peroxidase |
Acc32503 | 362.30 b | 390.84 b | 777.05 a | POD | PAL | 过氧化物酶超家族蛋白Peroxidase superfamily protein |
Acc32502 | 219.54 b | 247.40 ab | 544.78 a | POD | PAL | 过氧化物酶超家族阴性蛋白Peroxidase superfamily negative protein |
Acc30435 | 47.41 b | 60.30 b | 623.97 a | POD | PAL | 木质素阴性过氧化物酶Lignin-forming anionic peroxidase |
Table 2 DEGs in disease resistance-related pathways
基因编号 Gene ID | 表达量 FPKM(‘21-2-4’) | KO | 途径 Pathway | NR注释 NR_annotation | ||
---|---|---|---|---|---|---|
0 h | 6 h | 24 h | ||||
Acc06866 | 33.27 b | 305.75 b | 2 308.34 a | PR1 | PTI、SA | 病程相关蛋白Pathogenesis-related protein |
Acc06865 | 64.83 b | 537.47 b | 2 678.93 a | PR1 | PTI、SA | 病程相关蛋白Pathogenesis-related protein |
Acc06864 | 367.59 b | 2 171.17 b | 10 865.11 a | PR1 | PTI、SA | 病程相关蛋白Pathogenesis-related protein |
Acc06484 | 14.20 b | 111.05 a | 25.56 b | FLS2 | PTI | LRR受体样激酶LRR receptor-like serine/threonine-protein kinase |
Acc25328 | 2.92 b | 15.19 a | 7.70 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
novel.6186 | 2.69 b | 12.26 a | 6.08 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
novel.36390 | 2.59 b | 11.14 a | 6.99 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
Acc25330 | 3.16 b | 12.70 a | 7.51 ab | EIX1_2 | PTI | 受体样蛋白Receptor-like protein |
Acc23527 | 2.24 b | 7.74 a | 10.43 ab | CPK | PTI | 钙依赖蛋白激酶Calcium-dependent protein kinase |
Acc18178 | 11.18 b | 30.83 a | 39.39 a | EDS1 | ETI | EDS1样蛋白 Protein EDS1B like |
Acc07305 | 28.87 b | 86.32 a | 89.80 a | CTSF | ETI | 半胱氨酸蛋白酶Cysteine protease |
Acc11129 | 78.04 b | 159.43 a | 305.97 a | CTSF | ETI | 半胱氨酸蛋白酶Cysteine protease |
Acc02718 | 10.53 b | 32.00 a | 31.63 a | RAR1 | ETI | 富含半胱氨酸和组氨酸结构域蛋白 Cysteine and histidine-rich domain-containing protein |
Acc21162 | 321.06 ab | 193.38 b | 692.83 a | PR1 | SA | 病程相关蛋白基本形式Basic form of pathogenesis-related protein like |
Acc29710 | 2.74 b | 5.29 ab | 11.16 a | 4CL | PAL | 类4香豆酸辅酶 A 4-coumarate-CoA ligase-like |
Acc01331 | 7.41 b | 23.73 a | 12.13 ab | 4CL | PAL | 4香豆酸辅酶 A 4-coumarate-CoA ligase |
Acc16561 | 160.02 b | 284.77 ab | 449.28 a | POD | PAL | 过氧化物酶Peroxidase |
Acc20997 | 1.91 b | 1.70 b | 10.94 a | POD | PAL | 过氧化物酶Peroxidase |
Acc00538 | 5.41 b | 28.43 a | 37.94 a | POD | PAL | 过氧化物酶Peroxidase |
Acc32503 | 362.30 b | 390.84 b | 777.05 a | POD | PAL | 过氧化物酶超家族蛋白Peroxidase superfamily protein |
Acc32502 | 219.54 b | 247.40 ab | 544.78 a | POD | PAL | 过氧化物酶超家族阴性蛋白Peroxidase superfamily negative protein |
Acc30435 | 47.41 b | 60.30 b | 623.97 a | POD | PAL | 木质素阴性过氧化物酶Lignin-forming anionic peroxidase |
Fig. 5 qRT-PCR validation of the expression levels of disease resistance candidate genes in kiwifruit resistant variety‘Jinnong’ and susceptible variety‘Hongsheng’after inoculation with Psa Different lowercase letters indicate significant differences between periods(P < 0.05).
Fig. 6 RNA-Seq and qRT-PCR results of differentially expressed genes in kiwifruit resistant monocot‘21-2-4’ and susceptible monocot‘19-3-11’inoculated with pathogenic bacterium Psa
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