Effects of AMF on Photosynthetic Characteristics and Gene Expressions of Tea Plants Under Drought Stress

doi:10.16420/j.issn.0513-353x.2023-0655

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (10): 2358-2370.doi: 10.16420/j.issn.0513-353x.2023-0655

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Effects of AMF on Photosynthetic Characteristics and Gene Expressions of Tea Plants Under Drought Stress

CHEN Xin¹, WU Xiaolong¹, LIU Shengrui², HU Xianchun¹^,^*(), LIU Chunyan¹^,²^,^*()

¹ College of Horticulture and Gardening，Yangtze University，Jingzhou，Hubei 434025，China
² Anhui Agricultural University，State Key Laboratory of Tea Plant Biology and Utilization，Hefei 230036，China

Received:2024-04-28 Revised:2024-07-16 Online:2024-10-25 Published:2024-10-21
Contact: HU Xianchun, LIU Chunyan

Abstract

Abstract:

In order to explore the effect of arbuscular mycorrhizal fungi（AMF）on the photosynthetic characteristics of tea plants under drought stress，the seedlings of Camellia sinensis. ‘Fuding Dabaicha’were subjected to water and AMF inoculation experiments under greenhouse pot conditions. The results showed that drought stress（55% of the maximum field water capacity）significantly inhibited the AMF infection on tea plant roots，reduced the chlorophyll b content，maximum quantum effect（QY_max）value，intercellular CO₂ concentration（C_i），stomatal vertical and horizontal diameter and stomatal opening，as well as down-regulated the expressions of carbon assimilation-related enzymes （CsRbcL，CsTK，CsFBPase，CsPRK）genes and chlorophyll synthesis-related enzyme gene CsHEME. Whereas，drought stress significantly increased the chlorophyll a/b and non-photochemical quenching （NPQ_lss）parameters，and up-regulated the expression of chlorophyll synthase enzymes gene CsHEMA1 in tea seedlings. Howbeit，under well-watered（75% of the maximum field water capacity）and drought stress conditions，inoculation with AMF significantly increased the content of chlorophyll a and total chlorophyll，net photosynthetic rate（P_n），stomatal conductance（G_s），C_i，and transpiration rate（T_r）but decreased NPQ_lss and stomatal density，accompany with the up-regulated expressions of carbon assimilation-related enzyme genes CsRbcL，CsTK，CsFBPase，CsPRK and chlorophyll synthesis-related enzyme genes CsHEMA1，CsHEMC，CsHEME，CsHEMG and CsCHLE in different degrees. Meanwhile，AMF inoculation also significantly increased the chlorophyll b content，QY_max，stomatal vertical，horizontal diameter and aperture under drought stress and chlorophyll a/b under well-watered. The results indicated that AMF can improve the adaptability of tea plant under drought stress by promoting photosynthesis，and the promotion effect was more significant under drought stress.

Key words: tea plant, arbuscular mycorrhizal fungi, drought stress, stomatal, photosynthetic characteristics

CHEN Xin, WU Xiaolong, LIU Shengrui, HU Xianchun, LIU Chunyan. Effects of AMF on Photosynthetic Characteristics and Gene Expressions of Tea Plants Under Drought Stress[J]. Acta Horticulturae Sinica, 2024, 51(10): 2358-2370.

Figures/Tables 10

References 45

[1]	Bi Y, Zhou H. 2021. Changes in peanut canopy structure and photosynthetic characteristics induced by an arbuscular mycorrhizal fungus in a nutrient-poor environment. Scientific Reports, 11 (1):14832. doi: 10.1038/s41598-021-94092-w pmid: 34290277
[2]	Bu Chunlan, Yan Meijing, Dong Tingfa, Liu Gang, Huang Gaiqun, Xu Xiao. 2022. Effects of arbuscular mycorrhizal fungi(AMF)on biomass,photosynthetic characteristics and infection rate of mulberry(Morus alba)in different combination groups. Journal of Plant Physiology, 58 (11):2181-2190. (in Chinese)
	补春兰, 晏梅静, 董廷发, 刘刚, 黄盖群, 胥晓. 2022. 接种丛枝菌根真菌(AMF)对不同性别合栽模式下桑树生物量、光合及侵染率的影响. 植物生理学报, 58 (11):2181-2190.
[3]	Chen S C, Zhao H J, Zou C C, Li Y S, Chen Y F, Wang Z H, Jiang Y, Liu A R, Zhao P Y, Wang M M, Ahammed G J. 2017. Combined inoculation with multiple arbuscular mycorrhizal fungi improves growth,nutrient uptake and photosynthesis in cucumber seedlings. Frontiers in Microbiology,8:2516.
[4]	Chen Y, Liu L, Guo Q, Zhu Z, Zhang L. 2016. Effects of different water management options and fertilizer supply on photosynthesis,fluorescence parameters and water use efficiency of Prunella vulgaris seedlings. Biological Research,49:e66259.
[5]	Cheng S, Zou Y N, Kuča K, Hashem A, Abd Allah E F, Wu Q S. 2021. Elucidating the mechanisms underlying enhanced drought tolerance in plants mediated by arbuscular mycorrhizal fungi. Frontiers in Microbiology,12:809473.
[6]	Chu Wei, Guo Xinlai, Zhang Chen, Zhou Liuting, Wu Zeyan, Lin Wenxiong. 2022. Research progress and future directions of arbuscular mycorrhizal fungi-plantrhizosphere microbial interaction. Journal of Chinese Eco-Agriculture, 30 (11):1709-1721. (in Chinese)
	储薇, 郭信来, 张晨, 周柳婷, 吴则焰, 林文雄. 2022. 丛枝菌根真菌-植物-根际微生物互作研究进展与展望. 中国生态农业学报, 30 (11):1709-1721.
[7]	Dai F J, Rong Z Y, Wu Q S, Abd-allah E F, Liu C Y, Liu S R. 2022. Mycorrhiza improves plant growth and photosynthetic characteristics of tea plants in response to drought stress. Biocell, 46 (5):1339-1346.
[8]	Dong Sangjie, Ge Shibei, Li Lan, He Liqun, Fan Feijun, Qi Zhenyu, Yu Jingquan, Zhou Yanhong. 2022. Effects of supplemental lighting on growth,root colonization by arbuscular mycorrhizal fungi and phosphorus uptake in pepper seedlings. Acta Horticulturae Sinica, 49 (8):1699-1712. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0604
	董桑婕, 葛诗蓓, 李岚, 贺丽群, 范飞军, 齐振宇, 喻景权, 周艳虹. 2022. 不同光质补光对辣椒幼苗生长、丛枝菌根共生和磷吸收的影响. 园艺学报, 49 (8):1699-1712. doi: 10.16420/j.issn.0513-353x.2021-0604
[9]	Fang Bijun, Zhuo Dinglong, Liu Xiaozhou, Deng Yanwen, Zeng Feng. 2023. Effects of drought stress and re-hydration on photosynthetic and chlorophyll fluorescence parameters of Melastoma candidum D. Don. Journal of Chinese Tropical Agriculture, 43 (2):44-49. (in Chinese)
	方必君, 卓定龙, 刘晓洲, 邓演文, 曾凤. 2023. 干旱胁迫及复水对野牡丹光合和叶绿素荧光参数的影响. 热带农业科学, 43 (2):44-49.
[10]	Ge Shibei, Jiang Xiaochun, Wang Lingyu, Yu Jingquan, Zhou Yanhong. 2020. Recent advances in the role and mechanism of arbuscular mycorrhiza-induced improvement of abiotic stress tolerance in horticultural plants. Acta Horticulturae Sinica, 47 (9):1752-1776. (in Chinese)
	葛诗蓓, 姜小春, 王羚羽, 喻景权, 周艳虹. 2020. 园艺植物丛枝菌根抗非生物胁迫的作用机制研究进展. 园艺学报, 47 (9):1752-1776.
[11]	Han Y, Lou X, Zhang W, Xu T, Tang M. 2022. Arbuscular mycorrhizal fungi enhanced drought resistance of Populus cathayana by regulating the 14-3- 3 family protein genes. Microbiology Spectrum, 10 (3):e0245621.
[12]	Hu L, Xiang L, Zhang L, Zhou X, Zou Z, Hu X. 2014. The photoprotective role of spermidine in tomato seedlings under salinity-alkalinity stress. PLoS ONE, 9 (10):e110855.
[13]	Jian Minfei, Wang Sichen, Yu Houping, Li Lingyu, Jian Meifeng, Yu Guanjun. 2016. Influence of Cd²⁺ or Cu²⁺ stress on the growth and photosynthetic fluorescence characteristics of Hydrilla verticillata. Acta Ecologica Sinica, 36 (6):1719-1727. (in Chinese)
	简敏菲, 汪斯琛, 余厚平, 李玲玉, 简美锋, 余冠军. 2016. Cd²⁺、Cu²⁺胁迫对黑藻(Hydrilla verticillata)的生长及光合荧光特性的影响. 生态学报, 36 (6):1719-1727.
[14]	Jiang Ying, Liu Xiongsheng, Li Juan, Yang Jisheng, Jiang Yi, Wang Renjie. 2019. Effects of arbuscular mycorrhizal fungi on the photosynthetic characteristics and fluorescence parameters of Tectona grandis seedlings under drought stress. Journal of Forest and Environment, 39 (6):608-615. (in Chinese)
	姜英, 刘雄盛, 李娟, 杨继生, 蒋燚, 王仁杰. 2019. 干旱胁迫下丛枝菌根真菌对柚木光合及荧光参数的影响. 森林与环境学报, 39 (6):608-615.
[15]	Li Ze, Tan Xiaofeng, Lu Kun, Zhang Lin, Long Hongxu, Lü Jiabin, Lin Qing. 2017. Influence of drought stress on the growth,leaf gas exchange,and chlorophyll fluorescence in two varieties of tung tree seedlings. Acta Ecologica Sinica, 37 (5):1515-1524. (in Chinese)
	李泽, 谭晓风, 卢锟, 张琳, 龙洪旭, 吕佳斌, 林青. 2017. 干旱胁迫对两种油桐幼苗生长、气体交换及叶绿素荧光参数的影响. 生态学报, 37 (5):1515-1524.
[16]	Liang Shengmin, Zhang Fei, Wu Qiangsheng. 2023. Arbuscular mycorrhizal fungi improve drought tolerance of trifoliate orange seedlings by regulating root polyamines. Acta Horticulturae Sinica, 50 (12):2680-2688. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-1036
	梁圣敏, 张菲, 吴强盛. 2023. 丛枝菌根真菌通过调节枳根系多胺提高抗旱性. 园艺学报, 50 (12):2680-2688. doi: 10.16420/j.issn.0513-353x.2022-1036
[17]	Liu C Y, Wang Y J, Wu Q S, Yang T Y, Kuca K. 2020. Arbuscular mycorrhizal fungi improve the antioxidant capacity of tea(Camellia sinensis)seedlings under drought stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48 (4):1993-2005.
[18]	Liu Hui, Chen Meng, Huang Yindi, Ren Jiahong, Fan Dongfang, Zhao Juan. 2017. Diversity of arbuscular mycorrhizal fungi in the rhizosphere of tea plant from Anhui tea area. Journal of Applied Ecolog,(9):2897-2906. (in Chinese)
	刘辉, 陈梦, 黄引娣, 任嘉红, 范东芳, 赵娟. 2017. 安徽茶区茶树丛枝菌根真菌多样性. 应用生态学报,(9):2897-2906. doi: 10.13287/j.1001-9332.201709.037
[19]	Liu Guohai, Ou Hanbiao, Wei Qiusi, Lu Zhifeng, Wei Shuoxing, Chen Dongying, Li Chaoguo, Li Songhai. 2023. Effects of drought stress on growth and photosynthetic characteristics of Albizia odoratissima seedlings. Guangxi Forestry Science, 52 (2):181-185. (in Chinese) doi: 10.19692/j.issn.1006-1126.20230206
	柳国海, 欧汉彪, 韦秋思, 卢志锋, 韦铄星, 陈冬颖, 李朝国, 李松海. 2023. 干旱胁迫对香合欢幼苗生长和光合特性的影响. 广西林业科学, 52 (2):181-185. doi: 10.19692/j.issn.1006-1126.20230206
[20]	Liu Peiming, Liu Chun. 2019. Effects of different cultivation modes on summer tea quality. China Tropical Agriculture, 87 (2):31-36. (in Chinese)
	刘胚明, 刘春. 2019. 不同栽培模式对夏季茶叶品质影响的研究. 中国热带农业, 87 (2):31-36.
[21]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-∆∆CT method. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[22]	Long Yan, Ma Minjuan, Wang Yingyun, Song Huaibo. 2021. The drought stress state of tomato in seedling stage was identified by chlorophyll fluorescence kinetic parameters. Transactions of the Chinese Society of Agricultural Engineering, 37 (11):72-179. (in Chinese)
	龙燕, 马敏娟, 王英允, 宋怀波. 2021. 利用叶绿素荧光动力学参数识别苗期番茄干旱胁迫状态. 农业工程学报, 37 (11):172-179.
[23]	Ma Rui, Lin Yong, Ma Tingting. 2022. Influences of arbuscular mycorrhizal fungi on quality and related gene expression of Liubao tea. Jiangsu Agricultural Sciences,(17):157-163. (in Chinese)
	马蕊, 林勇, 马婷婷. 2022. 丛枝菌根真菌对六堡茶茶叶品质及其相关基因表达的影响. 江苏农业科学,(17):157-163.
[24]	Mathur S, Tomar R S, Jajoo A. 2019. Arbuscular mycorrhizal fungi(AMF)protects photosynthetic apparatus of wheat under drought stress. Photosynthesis Research,139:227-238.
[25]	Porcel R, Redondo-Gomez S, Mateos-Naranjo E, Aroca R, Garcia R, Ruiz-Lozano J M. 2015. Arbuscular mycorrhizal symbiosis ameliorates the optimum quantum yield of photo system Ⅱ and reduces non-photochemical quenching in rice plants subjected to salt stress. Journal of Plant Physiology,185:75-83.
[26]	Qian W, Hu J, Zhang X, Zhao L, Wang Y. 2018. Response of tea plants to drought stress// Stress physiology of tea in the face of climate change. Singapore:Springer:63-81.
[27]	Ren C G, Kong C C, Wang S X, Xie Z H. 2019. Enhanced phytoremediation of uranium-contaminated soils by arbuscular mycorrhiza and rhizobium. Chemosphere,217:773-779.
[28]	Shan D, Wang C, Song H, Bai Y, Zhang H, Hu Z, Wang L, Shi K, Zheng X, Yan T, Sun Y, Zhu Y, Zhang T, Zhou Z, Guo Y, Kong J. 2021. The MdMEK2-MdMPK6-MdWRKY 17 pathway stabilizes chlorophyll levels by directly regulating MdSUFB in apple under drought stress. The Plant Journal, 108 (3):814-828.
[29]	Shao Y D, Zhang D J, Hu X H, Wu Q S, Kuča K. 2018. Mycorrhiza-induced changes in root growth and nutrient absorption of tea plants. Plant Soil & Environment, 64 (6):283-289.
[30]	Shao Y D, Zhang D J, Hu X C, Wu Q S, Jiang C J, Gao X B, Kuča K. 2019. Arbuscular mycorrhiza improves leaf food quality of tea plants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47 (3):608-614.
[31]	Sharma M P, Grover M, Chourasiya D, Bharti A, Agnihotri R, Maheshwari H S, Pareek A, Buyer J S, Sharma S K, Schütz L, Mathimaran N, Singla-Pareek S L, Grossman J M, Bagyaraj D J. 2020. Deciphering the role of trehalose in tripartite symbiosis among rhizobia,arbuscular mycorrhizal fungi,and legumes for enhancing abiotic stress tolerance in crop plants. Frontiers in Microbiology,11:509919.
[32]	Song Fengping, Meng Zuqing. 2018. Research progress on photosynthetic parameters of crops in drought stress. Journal of Plateau Agriculture, 2 (2):138-144,212. (in Chinese)
	宋丰萍, 蒙祖庆. 2018. 干旱胁迫下作物光合参数研究进展. 高原农业, 2 (2):138-144,212.
[33]	Sun Jun, Zhu Liugang, Lin Zhikun, Zhang Wenjin. 2015. Research process on photosynthesis of tea plants. Fujian Journal of Agricultural Science, 30 (12):1231-1237. (in Chinese)
	孙君, 朱留刚, 林志坤, 张文锦. 2015. 茶树光合作用研究进展. 福建农业学报, 30 (12):1231-1237.
[34]	Sun Qilu. 2019. Effect of re-hydration method on tea plant under drought stress and cloning and expression of CsSnRK2 gene family[M. D. Dissertation]. Anhui: Anhui Agricultural University. (in Chinese)
	孙琪璐. 2019. 复水方式对干旱胁迫下茶树的影响及CsSnRK2基因家族的克隆与表达[硕士论文]. 安徽: 安徽农业大学.
[35]	Tyagi J, Tyagi J, Varma A, Pudake R N. 2017. Evaluation of comparative effects of arbuscular mycorrhiza(Rhizophagus intraradices)and endophyte(Piriformospora indica)association with finger millet(Eleusine coracana)under drought stress. European Journal of Soil Biology,81:1-10.
[36]	Upadhyaya H, Panda S K, Dutta B K. 2008. Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery. Acta Physiology Plant,30:457-468.
[37]	Wang Pingrong, Zhang Fantao, Gao Jiaxu, Sun Xiaoqiu, Deng Xiaojian. 2009. An overview of chlorophyll biosynthesis in higher plants. Acta Botanica Boreali-Occidentalia Sinica, 29 (3):629-636. (in Chinese)
	王平荣, 张帆涛, 高家旭, 孙小秋, 邓晓建. 2009. 高等植物叶绿素生物合成的研究进展. 西北植物学报, 29 (3):629-636.
[38]	Wang S J, Ren Y, Han L N, Nie Y Y, Zhang S Y, Xie X N, Hu W T, Chen H, Tang M. 2023. Insights on the impact of arbuscular mycorrhizal symbiosis on eucalyptus grandis tolerance to drought stress. Microbiology Spectrum, 11 (2):e0438122.
[39]	Wang W X, Zhang F, Chen Z L, Liu J, Guo C, He J D, Zou Y N, Wu Q S. 2017. Responses of phytohormones and gas exchange to mycorrhizal colonization in trifoliate orange subjected to drought stress. Archives of Agronomy and Soil Science,63:14-23.
[40]	Wang Xuekui. 2006. Experimental principles and techniques of plant physiology and biochemistry 2nd ed. Beijing: Higher Education Press. (in Chinese)
	王学奎. 2006. 植物生理生化实验原理和技术. 2版. 北京: 高等教育出版社.
[41]	Wang Yujuan, Gao Xiubing, Wu Qiangsheng, Ji Daobao, Cai Fan, Liu Chunyan. 2020. Influences of arbuscular mycorrhizal fungi on plant growth and tea quality of Fuding dabaicha seedlings under different water conditions. Journal of Tea Science, 40 (5):588-596. (in Chinese)
	王玉娟, 高秀兵, 吴强盛, 纪道保, 蔡樊, 刘春艳. 2020. 不同水分条件下AM真菌对福鼎大白茶生长和茶叶品质的影响. 茶叶科学, 40 (5):588-596.
[42]	Xia Zhenhua, Chen Yaning, Zhu Chenggang, Zhou Yingying, Chen Xiaolin. 2018. Stomatal change in leaves of Populus euphratica under drought stress. Arid Zone Research, 35 (5):1111-1117. (in Chinese) doi: 10.13866/j.azr.2018.05.14
	夏振华, 陈亚宁, 朱成刚, 周莹莹, 陈晓林. 2018. 干旱胁迫环境下的胡杨叶片气孔变化. 干旱区研究, 35 (5):1111-1117. doi: 10.13866/j.azr.2018.05.14
[43]	Xie Y, Yang L, He Z. 2019. Effects of AMF infection on photosynthetic characteristics of tomato under salt stress. 2019 5th International Conference on Energy Materials and Environment Engineering,doi:10.1088/1755-1315/295/2/012077.
[44]	Yang Ni, Wan Yiwen, Li Yimin, Han Miaohua, Teng Ruimin, Liu Jiexia, Zhuang Jing. 2022. Effects of exogenous spermidine on photosynthetic characteristics and gene expression of key enzymes under salt stress in tea plant. Acta Horticulturae Sinica, 49 (2):378-394. (in Chinese) doi: 10.16420/j.issn.0513-353x.2020-0946
	杨妮, 万绮雯, 李逸民, 韩妙华, 滕瑞敏, 刘洁霞, 庄静. 2022. 外源亚精胺对盐胁迫下茶树光合特性及关键酶基因表达的影响. 园艺学报, 49 (2):378-394. doi: 10.16420/j.issn.0513-353x.2020-0946
[45]	Zahoor R, Dong H, Abid M, Zhao W Q, Wang Y H, Zhou Z G. 2017. Potassium fertilizer improves drought stress alleviation potential in cotton by enhancing photosynthesis and carbohydrate metabolism. Environmental and Experimental Botany,37:73-83.

基因 Gene	名称 Name	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Reverse primer sequence
β-actin	肌动蛋白Actin	GGCGGATCAAGTGTTGGAAGGGAG	ACGCTTGGGATTGTATTCGGCATTA
CsCHLM	Mg-原卟啉Ⅸ甲基转移酶Magnesium protoporphyrin Ⅸ methyltransferase	CTCTATTGCCTCATTCCTC	ATTTAGTGTTTGGGTTGGT
CsCHLE	Mg-原卟啉Ⅸ单甲基酯环化酶Magnesium protoporphyrin Ⅸ monomethyl ester cyclase	CAATGACTGGAAGGCTAA	ATTCTTTGGTGTTGAGGC
CsHEMA1	谷氨酸-tRNA还原酶 Glutamyl-tRNA reductase	ATTCGTTGCGAGATTGTT	GCTGCTCCTTTCCTTTGT
CsHEMC	胆色素原脱氨酶 Porphobilinogen deaminase	TGACCGCCATTCTTTCTA	GCTAATCTTGTTTCCTCGT
CsHEMD	尿卟啉原Ⅲ合成酶 Uroporphyrinogen Ⅲ synthase	TGTCTGGGCTGTCTTCGA	CAAATCAGGCAACCGTGT
CsHEME	尿卟啉原Ⅲ脱羧酶 Uroporphyrinogen Ⅲ decarboxylase	ACATTCGCTTCTGTTCCC	TTTCTACTTCCAGCCCTC
CsHEMG	原卟啉原氧化酶Menaquinone dependent protoporphyrinogen oxidase	TCTGTGGAAGAAACGGAACT	CCGCAACGAAAGGGTCAA
CsPPOX	原卟啉Ⅳ原氧化酶 Protoporphyrinogen Ⅳ oxidase	GAAGCAGTTGACCGTGAC	AAAGCCCTCTGAACCCAC
CsRbcL	核酮糖-1,5-二磷酸羧化/加氧酶大亚基Ribulose- 1,5-bisphosphate carboxylase/ oxygenase large subunit	TGGCTGGAGATGGGACGA	CCTCTGGTAATCAGAACAGGGTT
CsTK	转酮醇酶 Transketolase	TGCCCAATGTTTTGATGCTACG	ATCCACCCTTTTCCACTCCCTC
CsFBPase	果糖-1,6-二磷酸酶 Furetose-1,6-bisphosphate phosphatase	GCAGATTGCTTCGTTGGTTC	TGCTATTATCCCTGTCCTCCC
CsPRK	核酮糖-5-磷酸激酶 Ribulose 5-phosphate kinase	AGTATTGGAGCCCGAAAGCC	CAAACAGGTAAACTGGGGTGAA

基因 Gene	名称 Name	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Reverse primer sequence
β-actin	肌动蛋白Actin	GGCGGATCAAGTGTTGGAAGGGAG	ACGCTTGGGATTGTATTCGGCATTA
CsCHLM	Mg-原卟啉Ⅸ甲基转移酶Magnesium protoporphyrin Ⅸ methyltransferase	CTCTATTGCCTCATTCCTC	ATTTAGTGTTTGGGTTGGT
CsCHLE	Mg-原卟啉Ⅸ单甲基酯环化酶Magnesium protoporphyrin Ⅸ monomethyl ester cyclase	CAATGACTGGAAGGCTAA	ATTCTTTGGTGTTGAGGC
CsHEMA1	谷氨酸-tRNA还原酶 Glutamyl-tRNA reductase	ATTCGTTGCGAGATTGTT	GCTGCTCCTTTCCTTTGT
CsHEMC	胆色素原脱氨酶 Porphobilinogen deaminase	TGACCGCCATTCTTTCTA	GCTAATCTTGTTTCCTCGT
CsHEMD	尿卟啉原Ⅲ合成酶 Uroporphyrinogen Ⅲ synthase	TGTCTGGGCTGTCTTCGA	CAAATCAGGCAACCGTGT
CsHEME	尿卟啉原Ⅲ脱羧酶 Uroporphyrinogen Ⅲ decarboxylase	ACATTCGCTTCTGTTCCC	TTTCTACTTCCAGCCCTC
CsHEMG	原卟啉原氧化酶Menaquinone dependent protoporphyrinogen oxidase	TCTGTGGAAGAAACGGAACT	CCGCAACGAAAGGGTCAA
CsPPOX	原卟啉Ⅳ原氧化酶 Protoporphyrinogen Ⅳ oxidase	GAAGCAGTTGACCGTGAC	AAAGCCCTCTGAACCCAC
CsRbcL	核酮糖-1,5-二磷酸羧化/加氧酶大亚基Ribulose- 1,5-bisphosphate carboxylase/ oxygenase large subunit	TGGCTGGAGATGGGACGA	CCTCTGGTAATCAGAACAGGGTT
CsTK	转酮醇酶 Transketolase	TGCCCAATGTTTTGATGCTACG	ATCCACCCTTTTCCACTCCCTC
CsFBPase	果糖-1,6-二磷酸酶 Furetose-1,6-bisphosphate phosphatase	GCAGATTGCTTCGTTGGTTC	TGCTATTATCCCTGTCCTCCC
CsPRK	核酮糖-5-磷酸激酶 Ribulose 5-phosphate kinase	AGTATTGGAGCCCGAAAGCC	CAAACAGGTAAACTGGGGTGAA

处理 Treatment	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b	叶绿素总含量 Total chlorophyll	叶绿素a/b Ratio of chlorophyll a/b
正常供水（对照）Well-watered（Control）	1.61 ± 0.07 c	0.65 ± 0.02 a	2.26 ± 0.10 c	2.48 ± 0.10 c
正常供水 + AMF Well-watered + AMF	1.85 ± 0.08 ab	0.68 ± 0.10 a	2.53 ± 0.17 a	2.72 ± 0.12 b
干旱胁迫 Drought stress	1.78 ± 0.03 b	0.46 ± 0.04 c	2.24 ± 0.04 c	3.87 ± 0.29 a
干旱胁迫 + AMF Drought stress + AMF	1.90 ± 0.07 a	0.55 ± 0.01 b	2.45 ± 0.08 b	3.45 ± 0.33 a

处理 Treatment	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b	叶绿素总含量 Total chlorophyll	叶绿素a/b Ratio of chlorophyll a/b
正常供水（对照）Well-watered（Control）	1.61 ± 0.07 c	0.65 ± 0.02 a	2.26 ± 0.10 c	2.48 ± 0.10 c
正常供水 + AMF Well-watered + AMF	1.85 ± 0.08 ab	0.68 ± 0.10 a	2.53 ± 0.17 a	2.72 ± 0.12 b
干旱胁迫 Drought stress	1.78 ± 0.03 b	0.46 ± 0.04 c	2.24 ± 0.04 c	3.87 ± 0.29 a
干旱胁迫 + AMF Drought stress + AMF	1.90 ± 0.07 a	0.55 ± 0.01 b	2.45 ± 0.08 b	3.45 ± 0.33 a

处理 Treatment	气孔密度/（个 · m^-2） Stomatal density	气孔横径/mm Stomatal vertical diameter	气孔纵径/mm Stomatal horizontal diameter	气孔开度/mm²Stomatal aperture
正常供水（对照）Well-watered	2.33 ± 0.21 a	0.175 ± 0.017 a	0.233 ± 0.015 a	0.032 ± 0.004 a
正常供水 + AMF Well watered + AMF	1.43 ± 0.12 c	0.184 ± 0.013 a	0.227 ± 0.014 ab	0.033 ± 0.004 a
干旱胁迫Drought stress	2.54 ± 0.19 a	0.147 ± 0.008 b	0.215 ± 0.010 b	0.025 ± 0.002 b
干旱胁迫 + AMF Drought stress + AMF	1.83 ± 0.19 b	0.172 ± 0.012 a	0.231 ± 0.009 a	0.031 ± 0.003 a

Effects of AMF on Photosynthetic Characteristics and Gene Expressions of Tea Plants Under Drought Stress

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 45

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Songyan, DIE Pengxiang, SONG Mengting, LI Zhijian, ZHOU Jian. Effect of Overexpression of Robinia pseudoacacia RpACBP3 Gene on Photosynthetic Physiological Characteristics of Nicotiana tabacum [J]. Acta Horticulturae Sinica, 2024, 51(9): 2155-2167.
[2]	WU Xiangqi, SUN Li, YU Zheping, YU Qinpei, LIANG Senmiao, ZHENG Xiliang, QI Xingjiang, ZHANG Shuwen. Functional Study of MrSPL4 Gene in Response to Drought and Low Temperature Stress in Chinese Bayberry [J]. Acta Horticulturae Sinica, 2024, 51(5): 927-938.
[3]	LIU Jinhong, WANG Zheng, YU Hao, XIN Yirui, QI Guoning, LIU Shenkui, REN Huimin. Identification of SLAC Gene Family in Phyllostachys edulis and Characterization of PheSLAC1 [J]. Acta Horticulturae Sinica, 2024, 51(3): 545-559.
[4]	XIA Hongyi, LIU Qiao, PENG Jiaqing, WU Wei, GONG Linzhong. Effects of f-Shaped Tree Shape on Photosynthetic Characteristics and Fruit Quality in‘Shine Muscat’Grapevines with Rain-Shelter Cultivation [J]. Acta Horticulturae Sinica, 2024, 51(3): 560-570.
[5]	YUAN Qingyun, HAN Yu, HE Wei, SU Hui, BAN Qiuyan, WU Chunlai, ZHOU Qiongqiong, XU Wenjing, WANG Liyuan, ZHANG Fen. Cloning and ABA Transport Function Analysis of CsNPF6.1/6.3 in Tea Plants [J]. Acta Horticulturae Sinica, 2024, 51(12): 2817-2828.
[6]	YANG Jiangshan, CHEN Yajuan, DAI Zibo, LI Dou, SHAO Zhang, JIN Xin, WANG Yuhang, WANG Chunheng. Effects of Potassium Fulvic Acid on Photosynthetic Characteristics and Fruit Quality of‘Cabernet Gernischt’Grape [J]. Acta Horticulturae Sinica, 2024, 51(12): 2843-2856.
[7]	LU Jianzeng, ZHOU Liyan, HUANG Xinyi, WU Fengzhi, GAO Danmei. Effects of Carbon Pool Strength on Plant Growth and Potassium Uptake of Tomato Intercropped with Potato-Onion [J]. Acta Horticulturae Sinica, 2024, 51(11): 2620-2632.
[8]	TIAN Jie, ZHOU Qianyi, TIE Yuanyu, SUN Haihong, and HUANG Sijie. Metabolomic Analysis of Garlic Seedling Under Drought Stress [J]. Acta Horticulturae Sinica, 2024, 51(1): 133-144.
[9]	PENG Hua, WANG Zhihui, YUE Cuinan, YANG Puxiang , LI Wenjin , TONG Zhongfei, and GUO Jin. A New Tea Cultivar‘Gancha 5’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 199-200.
[10]	WANG Leigang, JIAO Xiaoyu, LIU Dandan, RUAN Xu, WU Qiong, and WANG Wenjie. A New Yellowish Tea Plant Cultivar‘Huohuang 1’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 203-204.
[11]	ZHANG Chen, LI Mengjie, YANG Xiaoxue, WANG Meiyun, XIAO Dong, WANG Jianjun, HOU Xilin, HU Jun, LIU Tongkun. The Creation and Study on Characteristics of New Material of Autotetraploid Purple Tsai-tai [J]. Acta Horticulturae Sinica, 2023, 50(7): 1419-1428.
[12]	LI Yuting, REN Lihui, WANG Yuan, ZHOU Aiying, YANG Wei, HUANG Jian. Photosynthetic Characteristics of Ziziphus jujuba‘Dongzao’Under Protected Cultivation [J]. Acta Horticulturae Sinica, 2023, 50(3): 647-656.
[13]	CHENG Qinghua, ZHANG Zhipeng, WU Yanping, WAN Yuhe, CHEN Yingjuan. Studies on the Antifungal Effect of Matrine on Colletotrichum Species on Tea Plant [J]. Acta Horticulturae Sinica, 2023, 50(2): 432-440.
[14]	LIU Hui, HUANG Ting, TAO Jianping, ZHANG Jiaqi, ZHANG Rongrong, SONG Liuxia, ZHAO Tongmin, YOU Xiong, XIONG Aisheng. Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato [J]. Acta Horticulturae Sinica, 2023, 50(10): 2079-2090.
[15]	YUE Cuinan, WANG Zhihui, YANG Puxiang, LI Wenjin, PENG Hua, CHEN Luojun, and ZHOU Hanzhong. A New Tea Cultivar‘Ningzhouzao 1’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 281-282.