Effect of Overexpression of Robinia pseudoacacia RpACBP3 Gene on Photosynthetic Physiological Characteristics of Nicotiana tabacum

doi:10.16420/j.issn.0513-353x.2023-0690

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (9): 2155-2167.doi: 10.16420/j.issn.0513-353x.2023-0690

• Cultivation · Physiology & Biochemistry • Previous Articles Next Articles

Effect of Overexpression of Robinia pseudoacacia RpACBP3 Gene on Photosynthetic Physiological Characteristics of Nicotiana tabacum

ZHANG Songyan¹, DIE Pengxiang¹, SONG Mengting¹, LI Zhijian¹, ZHOU Jian¹^,²^,^*()

¹ School of Horticulture and Landscape Architecture，Henan Institute of Science and Technology，Xinxiang，Henan 453003，China
² Henan Province Engineering Center of Horticultural Plant Resource Utilization and Germplasm Enhancement，Xinxiang，Henan 453003，China

Received:2023-11-26 Revised:2024-04-01 Online:2024-09-25 Published:2024-09-19
Contact: ZHOU Jian

Abstract

Abstract:

To investigate the regulatory effects of RpACBP3 from Robinia pseudoacacia on the photosynthetic-physiological functions of Nicotiana tabacum，this gene was transferred into N. tabacum‘NC89’via Agrobacterium-mediated method，and the relevant indices of RpACBP3 over-expressing tobacco plants were measured using wild-type plants as the control. The results showed that seven strains of N. tabacum with RpACBP3 over-expression were obtained in this experiment. Compared to those in wild-type plants，chlorophyll a，chlorophyll b，and total chlorophyll content considerably increased and carotenoid content decreased in RpACBP3 over-expression strains. Additionally，some indices such as initial fluorescence（F_o），non-photochemical quenching coefficient（q_N）decreased，maximum photochemical efficiency（F_v/F_m），actual photochemical quantum efficiency（Φ_PSII），photosynthetic rate （P_n），and intercellular CO₂ concentration（C_i）increased，whereas others such as stomatal conductance（G_s），transpiration rate（T_r）decreased，stomatal length，and stomatal area increased in RpACBP3-over- expressing-strains. Correlation analysis revealed significant correlations between most of the indicators，and random forest regression showed that RpACBP3 overexpression had the greatest effects on tobacco indices such as chlorophyll a content，Φ_PSII，C_i，and stomatal area，respectively. In summary，the RpACBP3 over-expression enhanced the light energy utilization of tobacco plants by increasing the chlorophyll content，improving the efficiency of light absorption and transformation by the photosystem，and promoting the stomatal development.

Key words: Robinia pseudoacacia, Nicotiana tabacum, RpACBP3, photosynthetic characteristics, stomata

ZHANG Songyan, DIE Pengxiang, SONG Mengting, LI Zhijian, ZHOU Jian. Effect of Overexpression of Robinia pseudoacacia RpACBP3 Gene on Photosynthetic Physiological Characteristics of Nicotiana tabacum[J]. Acta Horticulturae Sinica, 2024, 51(9): 2155-2167.

Figures/Tables 10

Table 1 The primers used in this experiment

用途 Function	引物名称 Primer name	序列（5′-3′） Sequence
转基因阳性苗鉴定Identification of transgenic positive seedlings	OE-RpACBP3	F：GAGAACACGGGGGACTCTAGAATGGAGCTTGTAACTGCAAGTG R：CGATCGGGGAAATTCGAGCTCTCATTTCTTTACATTGTTCTC
qRT-PCR	NtActin	F：ACCTCTATGGCAACATTGTGCTCAG R：CTGGGAGCCAAAGCGGTGATT
qRT-PCR	qRpACBP3	F：ACCGGTACACGGTTCAAACA R：GACAATGTCTGGTTTCGCCG

Fig. 1 PCR assay of Nicotiana tabacum-transformed Robinia pseudoacacia RpACBP3 strains M：DNA marker DL2000；P：Positive plasmid control；N：Negative control；WT：Wild type plants；OE：Positive lines. The same below.

Fig. 2 qRT-PCR detection of Nicotiana tabacum trans-Robinia pseudoacacia RpACBP3 strains（OE）and wild type（WT） Different lowercase letters represent the significant difference between the lines（P < 0.05）. The same below.

Fig. 3 Chlorophyll and carotenoid content of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

Fig. 4 Chlorophyll fluorescence characterization of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

Fig. 5 Changes in photosynthetic function of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

Table 2 The in stomatal morphology of Nicotiana tabacum turned Robinia pseudoacacia RpACBP3 strain（OE）and wild type（WT）

株系 Lines	长度/μm Length	宽度/μm Width	面积/μm² Area
WT	17.87 ± 1.10 b	8.42 ± 0.65 bc	92.18 ± 13.78 c
OE1	19.27 ± 1.15 ab	8.29 ± 0.44 c	129.73 ± 9.12 abc
OE3	19.36 ± 0.18 ab	8.31 ± 0.22 c	118.34 ± 6.46 bc
OE5	21.35 ± 0.44 a	8.45 ± 0.20 bc	144.81 ± 10.84 ab
OE6	19.76 ± 0.38 ab	8.96 ± 0.55 abc	143.18 ± 6.47 ab
OE9	21.29 ± 0.56 a	9.79 ± 0.30 ab	159.23 ± 15.25 ab
OE10	21.80 ± 1.27 a	10.33 ± 0.63 a	175.04 ± 29.95 a
OE12	20.30 ± 0.74 ab	9.70 ± 0.19 abc	143.35 ± 6.35 ab

Table 3 Principal component analysis of indicators in Nicotiana tabacum transgenic Robinia pseudoacacia RpACBP3 strains %

指标 Index	第1主成分 Prin 1	第2主成分 Prin 2	第3主成分 Prin 3	第4主成分 Prin 4	第5主成分 Prin 5
方差贡献率Variance contribution rate	43.56	24.27	10.72	6.88	4.48
累计贡献率Cumulative contribution rate	43.56	67.83	78.55	85.43	89.91

Fig. 6 PCA（a）and random forest regression analysis（b）of indicators in Nicotiana tabacum transgenic Robinia pseudoacacia RpACBP3 strains

Fig. 7 Correlation（a）and cluster heat map（b）of indicators in Nicotiana tabacum transgenic Robinia pseudoacacia RpACBP3 strains In figure a，red represents positively correlated，and blue represents negatively correlated. The darker the color，the larger the correlation.

References 40

[41]	Yu Hongyan, Sun Mei, Chen Hongyi, Liu Zhenya, Yang Hangmei. 2023. Functional adaptation of a lakeside plant Scirpus validus in northwest Yunnan. Journal of Northeast Forestry University, 51 (1):45-53,60. (in Chinese)
	余洪艳, 孙梅, 陈弘毅, 刘振亚, 杨航美. 2023. 滇西北湖滨带植物水葱的功能适应性. 东北林业大学学报, 51 (1):45-53,60.
[42]	Zhang Xiaojing. 2022. Physiological characteristics and RpACBP3 gene transformation of Robinia pseudoacacia tissue culture seedlings under lead stress[M. D. Dissertation]. Xinxiang: Henan Institute of Science and Technology. (in Chinese)
	张晓静. 2022. 铅胁迫下刺槐组培苗生理特性及RpACBP3基因转化研究[硕士论文]. 新乡: 河南科技学院.
[43]	Zhou J, Jiang Z P, Ma J, Yang L F, Wei Y. 2017. The effects of lead stress on photosynthetic function and chloroplast ultrastructure of Robinia pseudoacacia seedlings. Environmental Science and Pollution Research, 24 (11):10718-10726.
[44]	Zhou Jian, Yang Lifeng, Hao Fengge, You Yang. 2009. Photosynthesis and chlorophyll-fluorescence of Magnolia grandiflora seedlings under low temperature stress. Acta Botanica Boreali-Occidentalia Sinica, 29 (1):136-142. (in Chinese)
	周建, 杨立峰, 郝峰鸽, 尤扬. 2009. 低温胁迫对广玉兰幼苗光合及叶绿素荧光特性的影响. 西北植物学报, 29 (1):136-142.
[45]	Zhou Yuanyuan. 2021. Molecular characterization,expression and functional analysis of Acyl-CoA-binding protein gene family in maize[M. D. Dissertation]. Jinan: University of Jinan. (in Chinese)
	周远远. 2021. ZmACBPs家族的分子鉴定、表达及功能分析[硕士论文]. 济南: 济南大学.
[1]	Bai Suhua, Zhu Jun, Dai Hongyi. 2012. Cloning and expression analysis of a new acyl-CoA-binding protein 2(MdACBP2)identified from Malus domestica. Acta Horticulturae Sinica, 39 (10):1893-1902. (in Chinese)
	柏素花, 祝军, 戴洪义. 2012. 苹果酰基辅酶A结合蛋白2编码基因MdACBP2的克隆和表达分析. 园艺学报, 39 (10):1893-1902.
[2]	Bao Yinmanda, Li Zhaojia, Liu Xiaodong, Wang Xu, Deng Wenjian, Zhou Guangyi, Pi Zhihao. 2023. Chlorophyll fluorescence characteristics and their photosynthetic capacity in response to diﬀerent provenance sources of Swida wilsoniana. Non-wood Forest Research, 41 (2):205-213. (in Chinese)
	宝音满达, 李兆佳, 刘晓东, 王旭, 邓文剑, 周光益, 皮志豪. 2023. 不同种源光皮树叶绿素荧光特性及其光合响应能力. 经济林研究, 41 (2):205-213.
[3]	Burton M, Rose T M, Faergeman N J, Knudsen J. 2005. Evolution of the Acyl-CoA-binding protein(ACBP). The Biochemical journal,392:299-307.
[4]	Cao Xiongjun, Han Jiayu, Cheng Guo, Wang Bo, Ma Guangren, Lin Ling, Tan Zongkun, Huang Qiumi, Chen Xiao, Chen Fuyi, Shi Xiaofang, Pan Fengping, Bai Xianjin. 2023. Effects of light duration and intensity on yield formation of‘Shine-Muscat’grape. Acta Horticulturae Sinica, 50 (8):1739-1746. (in Chinese)
	曹雄军, 韩佳宇, 成果, 王博, 马广仁, 林玲, 谭宗琨, 黄秋秘, 陈潇, 陈孚仪, 时晓芳, 盘丰平, 白先进. 2023. 光照时数及强度对‘阳光玫瑰’葡萄产量形成的影响. 园艺学报, 50 (8):1739-1746.
[5]	Chen Y Z, Fu M C, Li H, Wang L G, Liu R Z, Liu Z J. 2023. Molecular characterization of the Acyl-CoA-binding protein genes reveals their significant roles in oil accumulation and abiotic stress response in cotton. Genes, 14 (4):859.
[6]	Du Z Y, Chen M X, Chen Q F, Xiao S, Chye M L. 2013. Arabidopsis Acyl-CoA-binding protein ACBP1 participates in the regulation of seed germination and seedling development. The Plant Journal, 74 (2):294-309.
[7]	Faergeman N J, Wadum M, Feddersen S, Burton M, Kragelund B B, Knudsen J. 2007. Acyl-CoA-binding proteins;structural and functional conservation over 2000 MYA. Molecular & Cellular Biochemistry, 299 (1-2):55-65.
[8]	Fan J J, Liu J, Culty M, Papadopoulos V. 2010. Acyl-coenzyme A binding domain containing 3(ACBD3;PAP7;GCP60):an emerging signaling molecule. Progress in Lipid Research, 49 (3):218-234.
[9]	Gao W, Li H Y, Xiao S, Chye M L. 2010. Protein interactors of Acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis. Plant Signaling & Behavior, 5 (8):1025-1027.
[10]	Gui Huiying, Fang Fazhi, Mai Youzhuan, Wu Erhuan, Zhang Xiaofeng. 2022. Effects of pruning intensity on growth and physiological characteristics of Hopea hainanensis seedlings and comprehensive evaluation. Journal of West China Forestry Science, 51 (6):79-85,100. (in Chinese)
	桂慧颖, 方发之, 麦有专, 吴二焕, 张晓凤. 2022. 修枝强度对坡垒幼树生长和生理特性的影响及综合评价. 西部林业科学, 51 (6):79-85,100.
[11]	Guo Z H, Haslam R, Michaelson L, Yeung E C, Lung S C, Napier J A, Chye M L. 2019. The overexpression of rice Acyl-CoA-binding protein 2 increases grain size and bran oil content in transgenic rice. The Plant Journal, 100 (6):1132-1147.
[12]	Guo Z H, Pogancev G, Meng W, Du Z Y, Pan L, Zhang R, Chye M L. 2021. The overexpression of rice Acyl-CoA-binding protein 4 improves salinity tolerance in transgenic rice. Environmental and Experimental Botany,183:104349.
[13]	Hills M J, Dann R, Lydiate D, Sharpe A. 1994. Molecular cloning of a cDNA from Brassica napus L. for a homologue of Acyl-CoA-binding protein. Plant Molecular Biology, 25 (5):917-920. pmid: 8075407
[14]	Hsiao A S. 2013. Functional analysis of cytosolic Acyl-coenzyme a-binding proteins in floral and seedling development[Ph. D. Dissertation]. Hong Kong: The University of Hong Kong.
[15]	Hu T H, Lung S C, Ye Z W, Chye M L. 2018. Depletion of Arabidopsis Acyl-CoA-binding protein 3 affects fatty acid composition in the phloem. Frontiers in Plant Science,9:2.
[16]	Hu Xuanping, Ji Chengjun, An Lihua. 2015. Leaf stomatic characteristics of dicotyledonous plants in the Tibetan Plateau grasslands. Acta Botanica Boreali-Occidentalia Sinica, 35 (7):1356-1366. (in Chinese)
	胡选萍, 吉成均, 安丽华. 2015. 青藏高原草地双子叶植物叶片的气孔特征研究. 西北植物学报, 35 (7):1356-1366.
[17]	Jin Yiting, Liu Wenhui, Liu Kaiqiang, Liang Guoling, Jia Zhifeng. 2022. Effect of water deficit stress on the chlorophyll fluorescence parameters of Avena sativa‘Qingyan No.1’over the whole crop growth period. Acta Prataculturae Sinica, 31 (6):112-126. (in Chinese) doi: 10.11686/cyxb2021154
	金祎婷, 刘文辉, 刘凯强, 梁国玲, 贾志锋. 2022. 全生育期干旱胁迫‘青燕1号’燕麦叶绿素荧光参数的影响. 草业学报, 31 (6):112-126. doi: 10.11686/cyxb2021154
[18]	Lai S H, Chye M L. 2021. Plant Acyl-CoA-binding proteins-their lipid and protein interactors in abiotic and biotic s tresses. Cells, 10 (5):1064.
[19]	Li Donglin, Wang Huo, Jiang Hao, Zhu Yayun, Jin Yaqin, Cui Mengfan. 2019. Effects of shading on photosynthetic characteristics and leaf anatomical structure of Emmenopterys henryi seedlings. Acta Ecologica Siniea, 39 (24):9089-9100. (in Chinese)
	李冬林, 王火, 江浩, 祝亚云, 金雅琴, 崔梦凡. 2019. 遮光对香果树幼苗光合特性及叶片解剖结构的影响. 生态学报, 39 (24):9089-9100.
[20]	Li Yaliang, Liu Ling, Li Changai, Wang Hui, Liu Haitao, Miao Guopeng. 2023. Analysis of carbon fixation,oxygen release characteristics and leaf stomatal micromorphology of 10 Rosaceae greening plants. Journal of Central South University of Forestry & Technology, 43 (8):102-112. (in Chinese)
	李亚亮, 刘玲, 李长爱, 王慧, 刘海涛, 缪国鹏. 2023. 10种蔷薇科绿化植物固碳释氧特性及叶片气孔微形态分析. 中南林业科技大学学报, 43 (8):102-112.
[21]	Lin L, Niu Z M, Jiang C D, Yu L, Wang H N, Qiao M Y. 2022. Influences of open-central canopy on photosynthetic parameters and fruit quality of apples(Malus × domestica)in the Loess Plateau of China. Horticultural Plant Journal, 8 (2):133-142.
[22]	Liu Bao, Chen Cunji, Lin Dading, Lin Sizu. 2014. Analyses of photosynthetic pigment content and chlorophyll fluorescence parameter in leaves of 21 provenances of Phoebe bournei. Acta Agriculturae Universitatis Jiangxiensis, 36 (1):115-121. (in Chinese)
	刘宝, 陈存及, 林达定, 林思祖. 2014. 21个闽楠种源叶片光合色素含量及叶绿素荧光参数分析. 江西农业大学学报, 36 (1):115-121.
[23]	Liu Yaoquan, Zhang Xiaoyang, Bai Bin. 2023. Comparative analysis of leaf stomatal density and morphology among wheat varieties of different ecotypes. Acta Agriculturae Boreali-occidentalis Sinica, 32 (5):677-684. (in Chinese)
	刘耀权, 张晓洋, 白斌. 2023. 不同生态型小麦品种叶片气孔密度及形态差异分析. 西北农业学报, 32 (5):677-684.
[24]	Ni Xin. 2021. Identification and functional analysis of PutACBP gene family in Puccinellia tenuiflora[M. D. Dissertation]. Harbin:Northeast Forestry University. (in Chinese)
	倪鑫. 2021. 星星草PutACBP基因家族鉴定及功能分析[硕士论文]. 哈尔滨: 东北林业大学.
[25]	Pu Xuan, Wang Linghui. 2023. Effects of nitrogen application levels on photosynthetic characteristics and drought resistance of four landscape tree species. Journal of West China Forestry Science, 52 (5):146-154. (in Chinese)
	蒲璇, 王凌晖. 2023. 施氮水平对4个景观树种光合特性及抗旱性影响. 西部林业科学, 52 (5):146-154.
[26]	Qi B B, Zhang X, Mao Z Q, Qin S J, Lv D G. 2023. Integration of root architecture,root nitrogen metabolism, and photosynthesis of‘Hanfu’apple trees under the cross-talk between glucose and IAA. Horticultural Plant Journal, 9 (4):631-644.
[27]	Qiao Jianlei, Yu Haiye, Song Shuyao, Xiao Yingkui, Zhang Lei, Zhang Yanping. 2013. Effects of nitrogen forms on photosynthetic pigment and chlorophyll fluorescence characteristics of potato leaves. Journal of China Agricultural University, 18 (3):39-44. (in Chinese)
	乔建磊, 于海业, 宋述尧, 肖英奎, 张蕾, 张艳平. 2013. 氮素形态对马铃薯叶片光合色素及其荧光特性的影响. 中国农业大学学报, 18 (3):39-44.
[28]	Qiao K, Wang M, Takano T, Liu S K. 2018. Overexpression of Acyl-CoA-binding protein 1(ChACBP1) from saline-alkali-tolerant Chlorella sp. enhances stress tolerance in Arabidopsis. Frontiers in Plant Science,9:1772.
[29]	Qin Peng-fei, Shang Xiaoguang, Song Jian, Guo Wangzhen. 2016. Genome-wide identification of Acyl-CoA-binding protein(ACBP)gene family and their functional analysis in abiotic stress tolerance in cotton. Acta Agronomica Sinica, 42 (11):1577-1591. (in Chinese) doi: 10.3724/SP.J.1006.2016.01577
	秦朋飞, 尚小光, 宋健, 郭旺珍. 2016. 棉花酰基辅酶A结合蛋白(ACBP)家族基因的发掘及在非生物胁迫抗性中的功能鉴定. 作物学报, 42 (11):1577-1591. doi: 10.3724/SP.J.1006.2016.01577
[30]	Qiu S, Zeng B. 2020. Advances in understanding the Acyl-CoA-binding protein in plants,mammals,yeast,and filamentous fungi. Journal of Fungi, 6 (1):34.
[31]	Sun Yanan, Zhao Yang, Zhao Yuanxiang, Cao Hai, Long Jianlei. 2021. Effects of drought and rewatering on photosynthetic characteristics and chlorophyll fluorescence of Trachycarpus fortunei seedlings. Journal of Central South University of Forestry & Technology, 41 (9):45-52. (in Chinese)
	孙娅楠, 赵杨, 赵渊祥, 曹海, 龙建磊. 2021. 棕榈幼苗光合和叶绿素荧光对干旱胁迫及复水的响应. 中南林业科技大学学报, 41 (9):45-52.
[32]	Takato H, Shimidzu M, Ashizawa Y, Takei H, Suzuki S. 2013. An Acyl-CoA-binding protein from grape that is induced through ER stress confers morphological changes and disease resistance in Arabidopsis. Journal of Plant Physiology, 170 (6):591-600.
[33]	Wen Jing, Hu Hongling, Chen Hong, Liu Xijian, Dai Dachuan, Zhang Ruyi. 2022. Response of photosynthetic physiology of Eucalyptus grandis saplings to cadmium stress. Journal of Northwest A & F University(Nat Sci Ed), 50 (3):30-39. (in Chinese)
	文静, 胡红玲, 陈洪, 刘喜建, 代大川, 张如义. 2022. 巨桉幼树对镉胁迫的光合生理响应. 西北农林科技大学学报(自然科学版), 50 (3):30-39.
[34]	Wei M, Su Y, Saunders R, Chye M L. 2011. The rice Acyl-CoA-binding protein gene family:phylogeny,expression and functional analysis. New Phytologist, 189 (4):1170-1184.
[35]	Xiao S, Chye M L. 2008. Arabidopsis ACBP1 overexpressors are Pb(II)-tolerant and accumulate Pb(II). Plant Signaling & Behavior, 3 (9):693-694.
[36]	Xiao S, Gao W, Chen Q F, Ramalingam S, Chye M L. 2010. Overexpression of membrane-associated Acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis. The Plant Journal, 54 (1):141-151.
[37]	Xu Shanshan, Tang Yin, Zhong Minghui, Li Lingyan, Wu Lihua, Lin Kaimin, Cao Guangqiu, Ye Yiquan. 2022. Effects of shading on the growth,photosynthetic characteristics and nutrient content of Aglaonema commutatum. Pratacultural Science, 39 (10):2083-2094. (in Chinese)
	许珊珊, 唐银, 钟明慧, 李玲燕, 伍丽华, 林开敏, 曹光球, 叶义全. 2022. 遮阴对粗肋草生长、光合特性和养分含量的影响. 草业科学, 39 (10):2083-2094.
[38]	Xue Fangting, Liu Xinliang, Liu Yuhua, Yu Pengfei, Yu Wanwen. 2023. Photosynthetic and chlorophyll fluorescence characteristics of gold-coloured mutant leaves of Ginkgo biloba. Journal of West China Forestry Science, 52 (4):129-136. (in Chinese)
	薛芳婷, 刘新亮, 刘玉华, 余鹏飞, 郁万文. 2023. 芽变黄叶银杏光合和叶绿素荧光特性. 西部林业科学, 52 (4):129-136.
[39]	Yao Xinzhuan, Lü Litang, Zhao Degang. 2016. Improving photosynthetic characteristics of tobacco plants with BAS1 gene in transgenic Arabidopsis thaliana. Journal of Mountain Agriculture and Biology, 35 (3):30-34,62. (in Chinese)
	姚新转, 吕立堂, 赵德刚. 2016. 转拟南芥BAS1基因提高烟草光合特性. 山地农业生物学报, 35 (3):30-34,62.
[40]	Ye X, Yu K, Gao Q M, Wilson E V, Navarre D, Kachroo P, Kachroo A. 2012. Acyl-CoA-binding proteins are required for cuticle formation and plant responses to microbes. Frontiers in Plant Science,3:224.

Effect of Overexpression of Robinia pseudoacacia RpACBP3 Gene on Photosynthetic Physiological Characteristics of Nicotiana tabacum

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 40

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XIA Hongyi, LIU Qiao, PENG Jiaqing, WU Wei, GONG Linzhong. Effects of f-Shaped Tree Shape on Photosynthetic Characteristics and Fruit Quality in‘Shine Muscat’Grapevines with Rain-Shelter Cultivation [J]. Acta Horticulturae Sinica, 2024, 51(3): 560-570.
[2]	YANG Jiangshan, CHEN Yajuan, DAI Zibo, LI Dou, SHAO Zhang, JIN Xin, WANG Yuhang, WANG Chunheng. Effects of Potassium Fulvic Acid on Photosynthetic Characteristics and Fruit Quality of‘Cabernet Gernischt’Grape [J]. Acta Horticulturae Sinica, 2024, 51(12): 2843-2856.
[3]	CHEN Xin, WU Xiaolong, LIU Shengrui, HU Xianchun, LIU Chunyan. Effects of AMF on Photosynthetic Characteristics and Gene Expressions of Tea Plants Under Drought Stress [J]. Acta Horticulturae Sinica, 2024, 51(10): 2358-2370.
[4]	ZHANG Chen, LI Mengjie, YANG Xiaoxue, WANG Meiyun, XIAO Dong, WANG Jianjun, HOU Xilin, HU Jun, LIU Tongkun. The Creation and Study on Characteristics of New Material of Autotetraploid Purple Tsai-tai [J]. Acta Horticulturae Sinica, 2023, 50(7): 1419-1428.
[5]	LI Yuting, REN Lihui, WANG Yuan, ZHOU Aiying, YANG Wei, HUANG Jian. Photosynthetic Characteristics of Ziziphus jujuba‘Dongzao’Under Protected Cultivation [J]. Acta Horticulturae Sinica, 2023, 50(3): 647-656.
[6]	LIU Hui, HUANG Ting, TAO Jianping, ZHANG Jiaqi, ZHANG Rongrong, SONG Liuxia, ZHAO Tongmin, YOU Xiong, XIONG Aisheng. Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato [J]. Acta Horticulturae Sinica, 2023, 50(10): 2079-2090.
[7]	PENG Yi, LI Yuanhui, YANG Rui, ZHANG Ziyi, LI Yanan, HAN Yunhao, ZHAO Wenchao, WANG Shaohui. The Jasmonic Acid Synthesis Gene LoxD Participates in the Regulation of Tomato Drought Resistance [J]. Acta Horticulturae Sinica, 2022, 49(2): 319-331.
[8]	YANG Ni, WAN Qiwen, LI Yimin, HAN Miaohua, TENG Ruimin, LIU Jiexia, ZHUANG Jing. Effects of Exogenous Spermidine on Photosynthetic Characteristics and Gene Expression of Key Enzymes Under Salt Stress in Tea Plant [J]. Acta Horticulturae Sinica, 2022, 49(2): 378-394.
[9]	ZHONG Zaofa, ZHANG Lijuan, GAO Sisi, PENG Ting. Leaf Cytological Characteristics and Resistance Comparison of Four Citrus Rootstocks Under Drought Stress [J]. Acta Horticulturae Sinica, 2021, 48(8): 1579-1588.
[10]	LIANG Qiaolan*，WEI Liexin，XU Bingliang，ZHANG Shuwu，and HAN Liang. Effect of the Trichoderma atroviride T2 Proteinaceous TraT2A Induction Treatment on Photoresponsive and Fluorescent Characteristics of Lily Leaves inoculated Botrytis cinerea [J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 769-778.
[11]	HU Shu，AN Yuyan，and WANG Liangju*. Ethylene is Involved in the Regulation of Stomatal Movement by ALA-ABA/dark in Apple Leaves [J]. ACTA HORTICULTURAE SINICA, 2020, 47(3): 409-420.
[12]	FANG Xia1，BAI Zhiliang1，ZHOU Shengcai2，ZHANG Lizhen2，WU Qiang 2，ZHANG Junhong1，and TONG Zaikang1,*. The Photosynthetic Characteristics of Phoebe zhennan f. elliptical and P. zhennan f. oblong [J]. ACTA HORTICULTURAE SINICA, 2019, 46(5): 964-974.
[13]	ZHOU Gaofeng，LI Bixian，FU Yanling，GUAN Guan，YAO Fengxian，and LIU Guidong*. Effects of Iron，Manganese and Zinc Deficiency on the Symptom，Photosynthetic Characteristics and Nutrient Status of‘Nanfeng’Tangerine [J]. ACTA HORTICULTURAE SINICA, 2019, 46(4): 691-700.
[14]	HU Jian，AN Yuyan，CAI Changyu，HE Shasha，and WANG Liangju*. Cytoplasmic pH is Involved in 5-aminolevulinic Acid（ALA）-induced Stomatal Opening in Apple Leaves [J]. ACTA HORTICULTURAE SINICA, 2019, 46(10): 1869-1881.
[15]	XIONG Lijun，AN Yuyan，and WANG Liangju*. The Role of Microtubule Skeleton and PP1/PP2A Protein Phosphatase in ALA-ABA Regulating Stomatal Movement in Apple Leaves [J]. ACTA HORTICULTURAE SINICA, 2018, 45(11): 2073-2088.