Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato

doi:10.16420/j.issn.0513-353x.2022-0627

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (10): 2079-2090.doi: 10.16420/j.issn.0513-353x.2022-0627

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato

LIU Hui¹, HUANG Ting¹, TAO Jianping¹, ZHANG Jiaqi¹, ZHANG Rongrong¹, SONG Liuxia³, ZHAO Tongmin³, YOU Xiong²^,^**(), XIONG Aisheng¹^,^**()

¹ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization，Key Laboratory of Horticultural Crop Biology and Germplasm Creation in East China of Ministry of Agriculture and Rural Affairs，College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China
² College of Sciences，Nanjing Agricultural University，Nanjing 210095，China
³ Institute of Vegetable Crops，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China

Received:2023-05-05 Revised:2023-08-03 Online:2023-10-25 Published:2023-10-30

Abstract

Abstract:

Circadian clock genes play important roles in the regulation of plant growth，development and metabolism. LNK1，EID1 and ELF3 are important circadian clock genes in tomato. In this study，orthologous genes of SlLNK1，SlEID1 and SlELF3 were identified by BLAST software in 35 species. The phylogenetic analysis found these genes were originated from land plants. qRT-PCR experiments showed that the three genes responded to photoperiod and different light intensities with different expression patterns. During two photoperiods（48 h），the net photosynthetic rate，intercellular CO₂ concentration，stomatal conductance，and transpiration rate changed rhythmically. Transcriptome analysis of temporal and spatial expression patterns of clock genes SlLNK1，SlEID1，and SlELF3 in tomato showed differences in different tissues.

Key words: tomato, circadian clock gene, light intensity, expression analysis, photosynthetic characteristics

LIU Hui, HUANG Ting, TAO Jianping, ZHANG Jiaqi, ZHANG Rongrong, SONG Liuxia, ZHAO Tongmin, YOU Xiong, XIONG Aisheng. Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato[J]. Acta Horticulturae Sinica, 2023, 50(10): 2079-2090.

Figures/Tables 10

References 30

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基因名称	基因号	引物（5′-3′）
Gene name	Gene ID	Primer
SlLNK1	Solyc04g009050	F：TCAGACTACGGTGGCTGGTA；R：TCCCGTGCTGACACTTTCAA
SlEID1	Solyc09g075080	F：AGTTCGACGTCTTCGTGTCC；R：GCCAAATGTGAACAAGGCGA
SlELF3	Solyc11g070100	F：AGATTCTGAGTTGCACGCCA；R：AAAATGAGGCTGAGGCCGAA
Tubulin	Solyc04g077020	F：TGACGAAGTCAGGACAGGAA；R：CTGCATCTTCTTTGCCACTG

基因名称	基因号	引物（5′-3′）
Gene name	Gene ID	Primer
SlLNK1	Solyc04g009050	F：TCAGACTACGGTGGCTGGTA；R：TCCCGTGCTGACACTTTCAA
SlEID1	Solyc09g075080	F：AGTTCGACGTCTTCGTGTCC；R：GCCAAATGTGAACAAGGCGA
SlELF3	Solyc11g070100	F：AGATTCTGAGTTGCACGCCA；R：AAAATGAGGCTGAGGCCGAA
Tubulin	Solyc04g077020	F：TGACGAAGTCAGGACAGGAA；R：CTGCATCTTCTTTGCCACTG

物种 Species	数据来源 Data sources	物种 Species	数据来源
物种 Species	数据来源 Data sources	物种 Species	Data sources
脐形紫菜Porphyra umbilicalis	Phytozome	藜麦Chenopodium quinoa	Phytozome
莱茵衣藻Chlamydomonas reinhardtii	Phytozome	甜菜Beta vulgaris	Phytozome
小立碗藓Physcomitrium patens	Phytozome	辣椒Capsicum chinense	NCBI
江南卷柏Selaginella moellendorffii	Phytozome	茄子Solanum melongena	Phytozome
无油樟Amborella trichopoda	Phytozome	番茄Solanum lycopersicum	Phytozome
睡莲Nymphaea colorata	Phytozome	向日葵Helianthus annuus	Phytozome
凤梨Ananas comosus	Phytozome	莴苣Lactuca sativa	Phytozome
玉米Zea mays	Phytozome	三七Panax stipuleanatus	NCBI
水稻Oryza sativa	Phytozome	西洋参Panax quinquefolium	NCBI
黄连Coptis chinensis	NCBI	人参Panax ginseng	NCBI
耧斗菜Aquilegia coerulea	Phytozome	竹节参Panax japonicus	NCBI
葡萄Vitis vinifera	Phytozome	龙牙楤木Aralia elata	NCBI
长寿花Kalanchoe laxiflora	Phytozome	水芹Oenanthe sinensis	Unpublished
黄瓜Cucumis sativus	Phytozome	鸭儿芹Cryptotaenia japonica	Unpublished
森林草莓Fragaria vesca	Phytozome	香菜Coriandrum sativum	NCBI
拟南芥Arabidopsis thaliana	Phytozome	芹菜Apium graveolens	NCBI
萝卜Raphanus sativus	NCBI	欧芹Petroselinum crispum	Unpublished
白菜Brassica rapa	Phytozome	胡萝卜Daucus carota	NCBI

物种 Species	数据来源 Data sources	物种 Species	数据来源
物种 Species	数据来源 Data sources	物种 Species	Data sources
脐形紫菜Porphyra umbilicalis	Phytozome	藜麦Chenopodium quinoa	Phytozome
莱茵衣藻Chlamydomonas reinhardtii	Phytozome	甜菜Beta vulgaris	Phytozome
小立碗藓Physcomitrium patens	Phytozome	辣椒Capsicum chinense	NCBI
江南卷柏Selaginella moellendorffii	Phytozome	茄子Solanum melongena	Phytozome
无油樟Amborella trichopoda	Phytozome	番茄Solanum lycopersicum	Phytozome
睡莲Nymphaea colorata	Phytozome	向日葵Helianthus annuus	Phytozome
凤梨Ananas comosus	Phytozome	莴苣Lactuca sativa	Phytozome
玉米Zea mays	Phytozome	三七Panax stipuleanatus	NCBI
水稻Oryza sativa	Phytozome	西洋参Panax quinquefolium	NCBI
黄连Coptis chinensis	NCBI	人参Panax ginseng	NCBI
耧斗菜Aquilegia coerulea	Phytozome	竹节参Panax japonicus	NCBI
葡萄Vitis vinifera	Phytozome	龙牙楤木Aralia elata	NCBI
长寿花Kalanchoe laxiflora	Phytozome	水芹Oenanthe sinensis	Unpublished
黄瓜Cucumis sativus	Phytozome	鸭儿芹Cryptotaenia japonica	Unpublished
森林草莓Fragaria vesca	Phytozome	香菜Coriandrum sativum	NCBI
拟南芥Arabidopsis thaliana	Phytozome	芹菜Apium graveolens	NCBI
萝卜Raphanus sativus	NCBI	欧芹Petroselinum crispum	Unpublished
白菜Brassica rapa	Phytozome	胡萝卜Daucus carota	NCBI

Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato

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