Identification and Bioinformatics Analysis of the HSF Family Gene in Pinellia ternata

doi:10.16420/j.issn.0513-353x.2023-0710

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (10): 2371-2385.doi: 10.16420/j.issn.0513-353x.2023-0710

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification and Bioinformatics Analysis of the HSF Family Gene in Pinellia ternata

YOU Qian, LIU Xiao, LIU Mengmeng, LIU Dan, BO Chen, ZHU Yanfang, DUAN Yongbo, XUE Jianping, ZHANG Aimin^*(), XUE Tao^*()

Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants，College of Life Sciences，Huaibei Normal University，Huaibei，Anhui 235000，China

Received:2024-05-14 Revised:2024-08-08 Online:2024-10-25 Published:2024-10-21
Contact: ZHANG Aimin, XUE Tao

Abstract

Abstract:

In this study，the PtHSF genes involved in the high-temperature-induced ST of Pinellia ternata were identified and characterized. Twenty-two members of the PtHSF gene family were accordingly identified in P. ternata，which were unevenly distributed across its 10 chromosomes. All PtHSF genes were found to contain HSF-characteristic domains（DBD conserved domains），and phylogenetic tree analysis revealed that these genes can be divided into three major subclasses. PtHSF genes in the same subclass were established to have similar gene structures and conserved motifs，and encode similar amino acids. Subcellular localization prediction indicated that 81.82% of the PtHSF protein is located in the nucleus，with the remainder being distributed outside the cell. Collinearity analysis revealed that the PtHSF gene family has only a single collinear pair，and that PtHSF and rice OsHSF have a close homologous evolutionary relationship. Expression pattern analysis revealed that the expression of PtHSF family members differs significantly among different plant tissues，with most members being observed to be highly expressed in roots and tubers. In response to exposure to high temperature stress，the induced expression of 12 PtHSF genes and inhibited expression of eight PtHSF genes were detected.

Key words: Pinellia ternata, HSF, bioinformatics, transcription factor, gene expression

YOU Qian, LIU Xiao, LIU Mengmeng, LIU Dan, BO Chen, ZHU Yanfang, DUAN Yongbo, XUE Jianping, ZHANG Aimin, XUE Tao. Identification and Bioinformatics Analysis of the HSF Family Gene in Pinellia ternata[J]. Acta Horticulturae Sinica, 2024, 51(10): 2371-2385.

Figures/Tables 10

References 51

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基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
PtHSF2	AACTTCTCCAGCTTCGTTCG	TCGCCCTTTTTGAAGCAGTC
PtHSF5	CAGCTCAACACCTATGGA	GGATGGTCTTCAGCAGAT
PtHSF7	TCACTCGTCTAACTCTTCC	CATTGCAGAGGTTCTTCAG
PtHSF8	TCATCTCCTGGAACGACGAC	AGCTGGAGAAGTTGTTGTGC
PtHSF9	AAGTCGGGAATTGCGACATC	AGTCCAGGAAGAGAGCAAGC
PtHSF15	ACTTCAAGCACGGCAACTTC	AACCCTTCGTTGGCAAACTC
PtHSF16	TGCAAGCAAGAAACGGAGAC	AGCATTGCTTTGGCTGCTTC
PtHSF18	CCCCAAGTACTTCAAGCACAAC	ACGATCTTCCGGAAACCGTAG
PtHSF19	ACATTCGAGATGGTGGAG	GGATGAAGGTGGAGAAGT
PtHSF20	TCACCAAGACGTACCAGTTGG	TGAAGCAGTCGTTGTTGTGC

基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
PtHSF2	AACTTCTCCAGCTTCGTTCG	TCGCCCTTTTTGAAGCAGTC
PtHSF5	CAGCTCAACACCTATGGA	GGATGGTCTTCAGCAGAT
PtHSF7	TCACTCGTCTAACTCTTCC	CATTGCAGAGGTTCTTCAG
PtHSF8	TCATCTCCTGGAACGACGAC	AGCTGGAGAAGTTGTTGTGC
PtHSF9	AAGTCGGGAATTGCGACATC	AGTCCAGGAAGAGAGCAAGC
PtHSF15	ACTTCAAGCACGGCAACTTC	AACCCTTCGTTGGCAAACTC
PtHSF16	TGCAAGCAAGAAACGGAGAC	AGCATTGCTTTGGCTGCTTC
PtHSF18	CCCCAAGTACTTCAAGCACAAC	ACGATCTTCCGGAAACCGTAG
PtHSF19	ACATTCGAGATGGTGGAG	GGATGAAGGTGGAGAAGT
PtHSF20	TCACCAAGACGTACCAGTTGG	TGAAGCAGTCGTTGTTGTGC

基因 Gene	CDS长度/bp CDS length	氨基酸数 Number of amino acids	分子量/kD Molecular weight	等电点 Isoelectric point	不稳定系数 Isoelectric point	脂溶系数 Fat solubility coefficient	亲水系数 Hydrophilicity coefficient	亚细胞定位 Subcellular localization
PtHSF1	1 206	401	44.79	5.22	48.70	65.69	-0.618	细胞核 Nucleus
PtHSF2	891	296	32.22	5.08	64.05	67.60	-0.670	细胞核 Nucleus
PtHSF3	1 767	588	64.51	5.23	67.80	66.38	-0.732	细胞外 Extracellular
PtHSF4	1 293	430	45.19	10.24	58.77	72.77	-0.342	细胞外 Extracellular
PtHSF5	1 065	354	38.99	4.78	65.20	69.55	-0.578	细胞核 Nucleus
PtHSF6	798	265	28.97	9.25	65.36	67.09	-0.639	细胞外 Extracellular
PtHSF7	1 098	365	39.83	5.35	78.99	58.36	-0.808	细胞核 Nucleus
PtHSF8	1 080	359	39.12	7.63	62.48	64.48	-0.685	细胞核 Nucleus
PtHSF9	759	252	26.26	4.91	78.68	61.59	-0.240	细胞外 Extracellular
PtHSF10	1 350	449	51.10	5.16	63.68	65.99	-0.845	细胞核 Nucleus
PtHSF11	1 506	501	54.56	4.88	63.56	70.04	-0.517	细胞核 Nucleus
PtHSF12	666	221	25.29	8.83	58.87	73.35	-0.726	细胞核 Nucleus
PtHSF13	1 329	442	47.04	6.62	61.51	64.75	-0.469	细胞核 Nucleus
PtHSF14	1 245	414	44.04	6.78	58.68	67.22	-0.348	细胞核 Nucleus
PtHSF15	1 017	338	38.30	5.24	59.85	64.32	-0.835	细胞核 Nucleus
PtHSF16	1 599	532	57.54	4.90	60.15	73.18	-0.493	细胞核 Nucleus
PtHSF17	990	329	36.39	8.95	56.12	72.37	-0.473	细胞核 Nucleus
PtHSF18	1 107	368	40.50	6.20	68.71	57.55	-0.818	细胞核 Nucleus
PtHSF19	1 188	395	44.06	5.00	59.81	82.66	-0.429	细胞核 Nucleus
PtHSF20	756	251	27.38	6.11	71.88	63.07	-0.738	细胞核 Nucleus
PtHSF21	1 290	429	49.44	5.20	60.05	71.75	-0.674	细胞核 Nucleus
PtHSF22	1 158	385	41.33	5.86	54.83	64.16	-0.498	细胞核 Nucleus

基因 Gene	CDS长度/bp CDS length	氨基酸数 Number of amino acids	分子量/kD Molecular weight	等电点 Isoelectric point	不稳定系数 Isoelectric point	脂溶系数 Fat solubility coefficient	亲水系数 Hydrophilicity coefficient	亚细胞定位 Subcellular localization
PtHSF1	1 206	401	44.79	5.22	48.70	65.69	-0.618	细胞核 Nucleus
PtHSF2	891	296	32.22	5.08	64.05	67.60	-0.670	细胞核 Nucleus
PtHSF3	1 767	588	64.51	5.23	67.80	66.38	-0.732	细胞外 Extracellular
PtHSF4	1 293	430	45.19	10.24	58.77	72.77	-0.342	细胞外 Extracellular
PtHSF5	1 065	354	38.99	4.78	65.20	69.55	-0.578	细胞核 Nucleus
PtHSF6	798	265	28.97	9.25	65.36	67.09	-0.639	细胞外 Extracellular
PtHSF7	1 098	365	39.83	5.35	78.99	58.36	-0.808	细胞核 Nucleus
PtHSF8	1 080	359	39.12	7.63	62.48	64.48	-0.685	细胞核 Nucleus
PtHSF9	759	252	26.26	4.91	78.68	61.59	-0.240	细胞外 Extracellular
PtHSF10	1 350	449	51.10	5.16	63.68	65.99	-0.845	细胞核 Nucleus
PtHSF11	1 506	501	54.56	4.88	63.56	70.04	-0.517	细胞核 Nucleus
PtHSF12	666	221	25.29	8.83	58.87	73.35	-0.726	细胞核 Nucleus
PtHSF13	1 329	442	47.04	6.62	61.51	64.75	-0.469	细胞核 Nucleus
PtHSF14	1 245	414	44.04	6.78	58.68	67.22	-0.348	细胞核 Nucleus
PtHSF15	1 017	338	38.30	5.24	59.85	64.32	-0.835	细胞核 Nucleus
PtHSF16	1 599	532	57.54	4.90	60.15	73.18	-0.493	细胞核 Nucleus
PtHSF17	990	329	36.39	8.95	56.12	72.37	-0.473	细胞核 Nucleus
PtHSF18	1 107	368	40.50	6.20	68.71	57.55	-0.818	细胞核 Nucleus
PtHSF19	1 188	395	44.06	5.00	59.81	82.66	-0.429	细胞核 Nucleus
PtHSF20	756	251	27.38	6.11	71.88	63.07	-0.738	细胞核 Nucleus
PtHSF21	1 290	429	49.44	5.20	60.05	71.75	-0.674	细胞核 Nucleus
PtHSF22	1 158	385	41.33	5.86	54.83	64.16	-0.498	细胞核 Nucleus

Identification and Bioinformatics Analysis of the HSF Family Gene in Pinellia ternata

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