Metabolomic Analysis of Garlic Seedling Under Drought Stress

doi:10.16420/j.issn.0513-353x.2022-0888

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (1): 133-144.doi: 10.16420/j.issn.0513-353x.2022-0888

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Metabolomic Analysis of Garlic Seedling Under Drought Stress

TIAN Jie¹^,², ZHOU Qianyi¹, TIE Yuanyu¹, SUN Haihong¹, and HUANG Sijie³^,^**()

¹ Key Laboratory of Qinghai-Tibetan Plateau Biotechnology of Ministry of Education，Academy of Agricultural and Forestry Sciences of Qinghai University，Xining 810016，China
² Laboratory for Research and Utilization of Germplasm Resources in Qinghai-Tibetan Plateau，Qinghai Academy of Agricultural and Forestry Sciences，Xining 810016，China
³ Nanjing Institute of Environmental Sciences，Ministry of Ecology and Environment，Nanjing 210042，China

Received:2023-07-17 Revised:2023-09-15 Online:2024-01-25 Published:2024-01-16
Contact: **（E-mail：hsjofrcc@126.com）

Abstract

Abstract:

Using high performance liquid chromatography tandem mass spectrometry extensively targeted metabolomics，garlic leaves metabolic components were detected and analyzed by principal component analysis，orthogonal partial least squares discriminant analysis，cluster analysis，enrichment analysis and correlation analysis. As a result，a total of 743 metabolites in 23 classes were identified，313 up-regulated and 430 down-regulated metabolites，with 134 differential metabolites，accounting for 18.03% of the overall metabolites. The highest number of up- and down-regulated differential metabolites were flavone，phenylpropanoids，flavonol，flavonoid，nucleotide and derivatives，amino acid and derivatives，carbohydrates，organic acid and derivatives，which were mainly enriched in the flavone and flavonol biosynthesis. The number of different metabolites of flavonoids in the leaves of garlic sprout after drought stress was higher and up-regulated. The metabolic pathways of garlic flavonoids were analyzed，and caffeate acid and tricin O-malonylhexoside were the most up-regulated metabolites. It was speculated that the increase of flavone and phenylpropanoids under drought stress significantly improved the drought resistance of garlic.

Key words: garlic, drought stress, metabolomics, flavone, phenylpropanoids

TIAN Jie, ZHOU Qianyi, TIE Yuanyu, SUN Haihong, and HUANG Sijie. Metabolomic Analysis of Garlic Seedling Under Drought Stress[J]. Acta Horticulturae Sinica, 2024, 51(1): 133-144.

Figures/Tables 8

References 29

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Metabolomic Analysis of Garlic Seedling Under Drought Stress

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代谢物种类 Metabolite species	总计 Sum	上调 Up	下调 Down	差异显著 Significant difference
黄酮Flavone	106	73	33	24
氨基酸及衍生物Amino acid and derivatives	97	30	67	20
有机酸及衍生物Organic acids and derivatives	85	30	55	10
脂质Lipids	70	20	50	4
核苷酸及衍生物Nucleotide and derivates	63	10	53	24
苯丙素Phenylpropanoids	59	38	21	7
生物碱Alkaloids	37	17	20	7
黄酮醇Flavonol	28	9	19	2
糖类Carbohydrates	20	4	16	14
维生素及衍生物Vitamins and derivatives	19	6	13	4
醇类Alcohols	17	10	7	2
花青素Anthocyanins	16	6	10	2
萜类Terpene	16	8	8	2
黄酮类Flavonoid	15	8	7	3
酚胺Phenolamides	15	6	9	2
黄烷酮Flavanone	13	5	8	1
吲哚及衍生物Indole derivatives	11	5	6	1
异黄酮Isoflavone	9	6	3	0
多酚Polyphenol	6	3	3	1
甾体Sterides	5	3	2	0
醌类Quinones	3	2	1	0
原花青素Proanthocyanidins	1	1	0	0
其他Others	32	13	19	4
总计Sum	743	313	430	134

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