AFLP Analysis of Genetic Resources of Dendrobium from China

doi:10.16420/j.issn.0513-353x.2021-0625

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1339-1350.doi: 10.16420/j.issn.0513-353x.2021-0625

• Research Papers • Previous Articles Next Articles

AFLP Analysis of Genetic Resources of Dendrobium from China

ZHANG Meng¹, SHAN Yuying¹, YANG Yebo¹, ZHAI Feifei², WANG Zhaoshan¹, JU Guansheng¹, SUN Zhenyuan¹, LI Zhenjian¹^,^*()

¹Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China
²School of Architectural and Artistic Design,Henan University of Technology,Jiaozuo,Henan 454000,China

Received:2022-03-13 Revised:2022-04-21 Online:2022-06-25 Published:2022-07-05
Contact: LI Zhenjian E-mail:zhenjianli@163.com

Abstract

Abstract:

Dendrobium plants are mainly distributed in Yunnan,Guizhou and Sichuan Province in China. China is the distribution center of Dendrobium plants in the world. In this study,Amplified Length Polymorphism（AFLP）was used to study the genetic diversity and phylogenetic relationship of 92 species of Dendrobium in China. Ten primer combinations were screened out from 24 pairs of primer combinations,and a total of 2 329 loci were amplified,among which 2 307 were polymorphic,accounting for 99.04% of the total loci. Phylogenetic analysis results showed that 92 Dendrobium samples were divided into four groups,and the genetic differentiation was significant. AFLP molecular markers supported that the two species of D. leptocladum and D. xichouense were classified into Sect. Dendrobium,and D. hookerianum was divided into Sect. Holochrysa. The results also showed that the shape and texture of the stem were important characteristics for the classification of Dendrobium,and the flower lip and enlargement of stem node were not the main factors for Dendrobium classification.

Key words: Orchidaceae, Dendrobium, AFLP, amplification, phylogenetic relationship, genetic diversity

CLC Number:

S682.31

ZHANG Meng, SHAN Yuying, YANG Yebo, ZHAI Feifei, WANG Zhaoshan, JU Guansheng, SUN Zhenyuan, LI Zhenjian. AFLP Analysis of Genetic Resources of Dendrobium from China[J]. Acta Horticulturae Sinica, 2022, 49(6): 1339-1350.

Figures/Tables 4

References 20

[1]	Agendae Academiae Sinicae Edita. 1999. Flora reipublicae popularis sinicae (Tomus 19). Beijing: Science Press. (in Chinese)
	中国科学院中国植物志编辑委员会. 1999. 中国植物志. 19卷. 北京: 科学出版社.
[2]	Bai Yin, Bao Yinghua, Wang Wenquan, Jiang Lili, Yan Yuning. 2007. Analysis of the phylogenetic relationship of Dendrobium in China by AFLP technique. Acta Horticulturae Sinica, 34 (6):1569-1574. (in Chinese)
	白音, 包英华, 王文权, 姜丽丽, 阎玉凝. 2007. 国产石斛属植物亲缘关系的AFLP分析. 园艺学报, 34 (6):1569-1574.
[3]	Chen S P, Ma L, Lan S R, Li M H. 2016. Morphological and molecular evidence for a new species from China:Dendrobium zhenghuoense (Epidendroideae;Orchidaceae). Phytotaxa, 275 (3):277-286. doi: 10.11646/phytotaxa.275.3.5 URL
[4]	Clements M A. 2006. Molecular phylogentics systematics in Dendrobieae(Orchidaceae). Aliso, 22 (1):465-480. doi: 10.5642/aliso.20062201.37 URL
[5]	Ding G, Xu G, Zhang W C, Lu S, Li X X, Gu S, Ding X Y. 2008. Preliminary geoherbalism study of Dendrobium officinale food by DNA molecular markers. European Food Research and Technology, 227 (4):1283-1286. doi: 10.1007/s00217-007-0812-8 URL
[6]	Feng S G, Jiang Y, Wang S, Jiang M Y, Chen Z, Ying Q C, Wang H Z. 2015. Molecular identification of Dendrobium species(Orchidaceae)based on the DNA barcode ITS 2 region and its application for phylogenetic study. International Journal of Molecular Sciences, 16 (9):21975-21988. doi: 10.3390/ijms160921975 URL
[7]	Flora of China Editorial Committee. 2009. Flora of China (Volume 29). Beijing: Science Press.
[8]	Jaime A, Silva T D, Jin X H, Dobránszki J, Lu J J, Wang H Z, Zotz G, Cardoso J C, Zeng S J. 2016. Advances in Dendrobium molecular research:applications in genetic variation,identification and breeding. Molecular Phylogenetics and Evolution, 95 (1):196-216. doi: 10.1016/j.ympev.2015.10.012 URL
[9]	Li Dan, Li Zhenjian, Mao Ping, Yan Xuefeng, Chun Ze, Ma Xinrong. 2012. Phylogenetic analysis and identification of Dendrobium species based on ribosomal DNA internal transcribed spacer(ITS)sequence. Acta Horticulturae Sinica, 39 (8):1539-1550. (in Chinese)
	栗丹, 李振坚, 毛萍, 严雪锋, 淳泽, 马欣荣. 2012. 基于ITS序列石斛材料的鉴定及系统进化分析. 园艺学报, 39 (8):1539-1550.
[10]	Li Haixia. 2012. Identify intraspecific and interspecific of the Dendrobium based on rDNA ITS sequence analysis[M. D. Dissertation]. Guangzhou: Guangzhou University of Chinese Medicine. (in Chinese)
	李海霞. 2012. 基于rDNA ITS序列分析的石斛属种内及种间鉴别[硕士论文]. 广州: 广州中医药大学.
[11]	Li Zhenjian, Wang Yuancheng, Han Bin, Yang Yebo, Wang Zhen, Sun Zhenyuan. 2019. Research progress on constituents of alkaloids in plants from Dendrobium Sw. Chinese Traditional and Herbal Drugs, 50 (13):3246-3254. (in Chinese)
	李振坚, 王元成, 韩彬, 杨业波, 王振, 孙振元. 2019. 石斛属植物生物碱成分研究进展. 中草药, 50 (13):3246-3254.
[12]	Lu Jiashi, Bu Zhaoyang, Lü Weili, Su Jianmu, Huang Changyan, Li Chunniu. 2013. ISSR analysis on genetic diversity of germplasms resources in Dendrobium Sw. from different habitats. Chinese Traditional and Herbal Drugs, 44 (1):96-100. (in Chinese)
	卢家仕, 卜朝阳, 吕维莉, 苏建睦, 黄昌艳, 李春牛. 2013. 不同产地石斛属种质资源的ISSR遗传多样性分析. 中草药, 44 (1):96-100.
[13]	Ren Yu, Wang Chengdan, Xu Shisong, Huang Shaohua, Zhang Yindong. 2013. Analysis of genetic relationship of ornamental Dendrobium by SSR marker. Chinese Journal of Tropical Crops, 34 (7):1252-1256. (in Chinese)
	任羽, 王呈丹, 徐世松, 黄少华, 张银东. 2013. 石斛兰亲缘关系的SSR分析. 热带作物学报, 34 (7):1252-1256.
[14]	Song Shuang, Zhou Yangfan, Liu Zhengjie, Zhao Mingfu, Yang Juan, Xu Shaozhong, Wen Guosong. 2016. Genetic diversity among Dendrobium with known origins based on the ISSR and AFLP markers. Journal of Yunnan Agricultural University(Natural Science Edition), 31 (4):688-695. (in Chinese)
	宋爽, 周洋帆, 刘正杰, 赵明富, 杨娟, 徐绍忠, 文国松. 2016. 利用ISSR和AFLP标记分析石斛种质资源的遗传多样性. 云南农业大学学报(自然科学版), 31 (4):688-695.
[15]	Takamiya T, Wongsawad P, Tajima N, Shioda N, Lu J F, Wen C L, Wu J B, Handa T, Iijima H, Kitanaka S. 2011. Identification of Dendrobium species used for herbal medicines based on ribosomal DNA internal transcribed spacer sequence. Biological & Pharmaceutical Bulletin, 34 (5):779-782.
[16]	Takamiya T, Wongsawad P, Sathapattayanon A, Tajima N, Suzuki S, Kitamura S, Shioda N, Handa T, Kitanaka S, Iijima H, Yukawa T. 2014. Molecular phylogenetics and character evolution of morphologically diverse groups,Dendrobium section Dendrobium and allies. AoB Plants, 6 (1):1-25.
[17]	Xiang X G, Mi X C, Zhou H L, Li J W, Chung S W, Li D Z, Huang W C, Jin W T, Li Z Y, Huang L Q. 2016. Biogeographical diversification of mainland Asian Dendrobium(Orchidaceae)and its implications for the historical dynamics of evergreen broad-leaved forests. Journal of Biogeography, 43 (7):1310-1323. doi: 10.1111/jbi.12726 URL
[18]	Xiang X G, Schuiteman A, Li D Z, Huang W C, Chung S W, Li J W, Zhou H L, Jin W T, Lai Y J, Li Z Y, Jin X H. 2013. Molecular systematics of Dendrobium(Orchidaceae,Dendrobieae)from mainland Asia based on plastid and nuclear sequences. Molecular Phylogenetics and Evolution, 69 (3):950-960. doi: 10.1016/j.ympev.2013.06.009 URL
[19]	Xu S Z, Li D Z, Li J W, Xiang X G, Jin W T, Huang W C, Jin X H, Huang L Q. 2015. Evaluation of the DNA barcodes in Dendrobium(Orchidaceae)from mainland Asia. PLoS ONE, 10 (1):e0115168. doi: 10.1371/journal.pone.0115168 URL
[20]	Ye M R, Hou B W, Luo J, Yan W J, Liu W, Ding X Y. 2015. Genetic diversity and conservation of the endangered herb Dendrobium moniliforme based on amplified fragment length polymorphism markers. Scientia Horticulturae, 189 (9):51-58. doi: 10.1016/j.scienta.2015.03.035 URL

引种地 Introducing site	种质 Species（cultivars）	引种地 Introducing site	种质 Species（cultivars）
云南景洪 Jinghong,Yunnan	长苏石斛 D. brymerianum	云南文山 Wenshan,Yunnan	翅梗石斛 D. trigonopus
	短棒石斛 D. capillipes		西畴石斛 D. xichouense
	草石斛 D. compactum	云南西双版纳 Xishuangbanna,Yunnan	黑毛石斛 D. williamsonii
	齿瓣石斛 D. devonianum	贵州兴义 Xingyi,Guizhou	钩状石斛 D. aduncum
	景洪石斛 D. exile		束花石斛 D. chrysanthum
	流苏石斛 D. fimbriatum		玫瑰石斛 D. crepidatum
	棒节石斛 D. findlayanum		叠鞘石斛 D. denneanum
	曲轴石斛 D. gibsonii		重唇石斛 D. hercoglossum
	杯鞘石斛 D. gratiosissimum		聚石斛 D. lindleyi
	苏瓣石斛 D. harveyanum		罗河石斛 D. lohohense
	疏花石斛 D. henryi		杓唇石斛 D. moschatum
	尖刀唇石斛 D. heterocarpum	贵州赤水 Chishui,Guizhou	金钗石斛 D. nobile
	金耳石斛 D. hookerianum	贵州铜仁 Tongren,Guizhou	梵净山石斛 D. fanjingshanense
	小黄花石斛 D. jenkinsii	广东始兴 Shixing,Guangdong	始兴石斛 D. shixingense
	长距石斛 D. longicornu	广西崇左 Chongzuo,Guangxi	檀香石斛 D. anosmum
	铁皮石斛 D. officinale	广西金秀 Jinxiu,Guangxi	矩唇石斛 D. linawianum
	少花石斛 D. parciflorum	广西凌云 Lingyun,Guangxi	美花石斛 D. loddigesii
	肿节石斛 D. pendulum	广西那坡 Napo,Guangxi	藏南石斛 D. monticola
	单葶草石斛 D. porphyrochilum	广西玉林 Yulin,Guangxi	密花石斛 D. densiflorum
	报春石斛 D. primulinum		滇桂石斛 D. guangxiense
	梳唇石斛 D. strongylanthum	安徽霍山 Huoshan,Anhui	霍山石斛 D. huoshanense
	叉唇石斛 D. stuposum	海南儋州 Danzhou,Hainan	竹枝石斛 D. salaccense
	广东石斛 D. wilsonii	海南广坝 Guangba,Hannan	广坝石斛 D. lagarum
	球花石斛 D. thyrsiflorum	海南琼中 Qiongzhong,Hainan	海南石斛 D. hainanense
	黄花石斛 D. dixanthum		华石斛 D. sinense
云南丽江 Lijiang,Yunnan	王亮石斛 D. wangliangii	河南南阳 Nanyang,Henan	曲茎石斛 D. flexicaule
云南勐腊 Mengla,Yunnan	勐腊石斛 D. chrysocrepis	河南南召 Nanzhao,Henan	河南石斛 D. henanense
	反瓣石斛 D. ellipsophyllum	四川夹江 Jiajiang,Sichuan	夹江石斛 D. jiajiangense
	针叶石斛 D. pseudotenellum	四川泸定 Luding,Sichuan	细叶石斛 D. hancockii
	勐海石斛 D. sinominutiflorum	台湾兰屿 Lanyu,Taiwan,China	燕石斛 D. equitans
	刀叶石斛 D. terminale		双花石斛 D. furcatopedicellatum
	大苞鞘石斛 D. wardianum		红花石斛 D. goldschmidtianum
	具槽石斛 D. sulcatum		吕宋石斛 D. luzonense
	喇叭唇石斛 D. lituiflorum		木石斛 D. crumenatum
	剑叶石斛 D. acinaciform	台湾台东Taidong,Taiwan,China	菱唇石斛 D. leptocladum
云南屏边 Pingbian,Yunnan	高山石斛 D. infundibulum	菲律宾 Philippines	紫晶石斛 D. amethystoglossum
	矮石斛 D. bellatulum		澳洲石斛 D. kingianum
	翅萼石斛 D. cariniferum	澳大利亚 Australia	黄喉石斛 D. signatum
	河口石斛 D. hekouense		宝石石斛 D. smillieae
云南文山 Wenshan,Yunnan	串珠石斛 D. falconeri	越南 Vietnam	黄玉石斛 D. topaziacum
	兜唇石斛 D. aphyllum	云南昆明 Kunming,Yunnan	红星 D.‘Akane’
	喉红石斛 D. christyanum		梦幻 D.‘Dream’
	线叶石斛 D. aurantiacum		火鸟 D.‘Fire Bird’
	鼓槌石斛 D. chrysotoxum		女王 D.‘Princess’
	晶帽石斛 D. crystallinum	海南三亚 Sanya,Hainan	树兰 Epidendrum secundum
	细茎石斛 D. moniliforme	海南昌江 Changjiang,Hainan	拟石斛 Oxystophyllum changjiangense
	紫瓣石斛 D. parishii	台湾台南 Tainan,Taiwan,China	武夷兰 Angraecum leonis
	紫婉石斛 D. transparens

引种地 Introducing site	种质 Species（cultivars）	引种地 Introducing site	种质 Species（cultivars）
云南景洪 Jinghong,Yunnan	长苏石斛 D. brymerianum	云南文山 Wenshan,Yunnan	翅梗石斛 D. trigonopus
	短棒石斛 D. capillipes		西畴石斛 D. xichouense
	草石斛 D. compactum	云南西双版纳 Xishuangbanna,Yunnan	黑毛石斛 D. williamsonii
	齿瓣石斛 D. devonianum	贵州兴义 Xingyi,Guizhou	钩状石斛 D. aduncum
	景洪石斛 D. exile		束花石斛 D. chrysanthum
	流苏石斛 D. fimbriatum		玫瑰石斛 D. crepidatum
	棒节石斛 D. findlayanum		叠鞘石斛 D. denneanum
	曲轴石斛 D. gibsonii		重唇石斛 D. hercoglossum
	杯鞘石斛 D. gratiosissimum		聚石斛 D. lindleyi
	苏瓣石斛 D. harveyanum		罗河石斛 D. lohohense
	疏花石斛 D. henryi		杓唇石斛 D. moschatum
	尖刀唇石斛 D. heterocarpum	贵州赤水 Chishui,Guizhou	金钗石斛 D. nobile
	金耳石斛 D. hookerianum	贵州铜仁 Tongren,Guizhou	梵净山石斛 D. fanjingshanense
	小黄花石斛 D. jenkinsii	广东始兴 Shixing,Guangdong	始兴石斛 D. shixingense
	长距石斛 D. longicornu	广西崇左 Chongzuo,Guangxi	檀香石斛 D. anosmum
	铁皮石斛 D. officinale	广西金秀 Jinxiu,Guangxi	矩唇石斛 D. linawianum
	少花石斛 D. parciflorum	广西凌云 Lingyun,Guangxi	美花石斛 D. loddigesii
	肿节石斛 D. pendulum	广西那坡 Napo,Guangxi	藏南石斛 D. monticola
	单葶草石斛 D. porphyrochilum	广西玉林 Yulin,Guangxi	密花石斛 D. densiflorum
	报春石斛 D. primulinum		滇桂石斛 D. guangxiense
	梳唇石斛 D. strongylanthum	安徽霍山 Huoshan,Anhui	霍山石斛 D. huoshanense
	叉唇石斛 D. stuposum	海南儋州 Danzhou,Hainan	竹枝石斛 D. salaccense
	广东石斛 D. wilsonii	海南广坝 Guangba,Hannan	广坝石斛 D. lagarum
	球花石斛 D. thyrsiflorum	海南琼中 Qiongzhong,Hainan	海南石斛 D. hainanense
	黄花石斛 D. dixanthum		华石斛 D. sinense
云南丽江 Lijiang,Yunnan	王亮石斛 D. wangliangii	河南南阳 Nanyang,Henan	曲茎石斛 D. flexicaule
云南勐腊 Mengla,Yunnan	勐腊石斛 D. chrysocrepis	河南南召 Nanzhao,Henan	河南石斛 D. henanense
	反瓣石斛 D. ellipsophyllum	四川夹江 Jiajiang,Sichuan	夹江石斛 D. jiajiangense
	针叶石斛 D. pseudotenellum	四川泸定 Luding,Sichuan	细叶石斛 D. hancockii
	勐海石斛 D. sinominutiflorum	台湾兰屿 Lanyu,Taiwan,China	燕石斛 D. equitans
	刀叶石斛 D. terminale		双花石斛 D. furcatopedicellatum
	大苞鞘石斛 D. wardianum		红花石斛 D. goldschmidtianum
	具槽石斛 D. sulcatum		吕宋石斛 D. luzonense
	喇叭唇石斛 D. lituiflorum		木石斛 D. crumenatum
	剑叶石斛 D. acinaciform	台湾台东Taidong,Taiwan,China	菱唇石斛 D. leptocladum
云南屏边 Pingbian,Yunnan	高山石斛 D. infundibulum	菲律宾 Philippines	紫晶石斛 D. amethystoglossum
	矮石斛 D. bellatulum		澳洲石斛 D. kingianum
	翅萼石斛 D. cariniferum	澳大利亚 Australia	黄喉石斛 D. signatum
	河口石斛 D. hekouense		宝石石斛 D. smillieae
云南文山 Wenshan,Yunnan	串珠石斛 D. falconeri	越南 Vietnam	黄玉石斛 D. topaziacum
	兜唇石斛 D. aphyllum	云南昆明 Kunming,Yunnan	红星 D.‘Akane’
	喉红石斛 D. christyanum		梦幻 D.‘Dream’
	线叶石斛 D. aurantiacum		火鸟 D.‘Fire Bird’
	鼓槌石斛 D. chrysotoxum		女王 D.‘Princess’
	晶帽石斛 D. crystallinum	海南三亚 Sanya,Hainan	树兰 Epidendrum secundum
	细茎石斛 D. moniliforme	海南昌江 Changjiang,Hainan	拟石斛 Oxystophyllum changjiangense
	紫瓣石斛 D. parishii	台湾台南 Tainan,Taiwan,China	武夷兰 Angraecum leonis
	紫婉石斛 D. transparens

接头 Adaptor	引物 Primer	序列（5′-3′） Sequence	引物 Primer	序列（5′-3′） Sequence
EcoR I-1		CTCGTAGACTGCGTACC	E83	GACTGCGTACCAATTCTCA
EcoR I-2		AATTGGTACGCAGTCTAC	M48	GATGAGTCCTGAGTAACAC
Mse I-1		GACGATGAGTCCTGAG	M49	GATGAGTCCTGAGTAACAG
Mse I-2		TACTCAGGACTCAT	M50	GATGAGTCCTGAGTAACAT
	EOO	GACTGCGTACCAATTC	M51	GATGAGTCCTGAGTAACCA
	MOO	GATGAGTCCTGAGTAA	M55	GATGAGTCCTGAGTAACGA
	E32	GACTGCGTACCAATTCAAC	M59	GATGAGTCCTGAGTAACTA
	E33	GACTGCGTACCAATTCAAG	M60	GATGAGTCCTGAGTAACTC
	E35	GACTGCGTACCAATTCACA	M61	GATGAGTCCTGAGTAACTG
	E37	GACTGCGTACCAATTCACG	M62	GATGAGTCCTGAGTAACTT
	E38	GACTGCGTACCAATTCACT	M84	GATGAGTCCTGAGTAATCC
	E75	GACTGCGTACCAATTCGTA

接头 Adaptor	引物 Primer	序列（5′-3′） Sequence	引物 Primer	序列（5′-3′） Sequence
EcoR I-1		CTCGTAGACTGCGTACC	E83	GACTGCGTACCAATTCTCA
EcoR I-2		AATTGGTACGCAGTCTAC	M48	GATGAGTCCTGAGTAACAC
Mse I-1		GACGATGAGTCCTGAG	M49	GATGAGTCCTGAGTAACAG
Mse I-2		TACTCAGGACTCAT	M50	GATGAGTCCTGAGTAACAT
	EOO	GACTGCGTACCAATTC	M51	GATGAGTCCTGAGTAACCA
	MOO	GATGAGTCCTGAGTAA	M55	GATGAGTCCTGAGTAACGA
	E32	GACTGCGTACCAATTCAAC	M59	GATGAGTCCTGAGTAACTA
	E33	GACTGCGTACCAATTCAAG	M60	GATGAGTCCTGAGTAACTC
	E35	GACTGCGTACCAATTCACA	M61	GATGAGTCCTGAGTAACTG
	E37	GACTGCGTACCAATTCACG	M62	GATGAGTCCTGAGTAACTT
	E38	GACTGCGTACCAATTCACT	M84	GATGAGTCCTGAGTAATCC
	E75	GACTGCGTACCAATTCGTA

引物 Primer	N_a	N_e	H	I	多态位点 Polymorphic loci	多态百分数/% Polymorphism percentage
E32-M48	1.9963	1.2604	0.1879	0.3211	269	99.63
E35-M48	1.9774	1.4286	0.2698	0.4234	260	97.74
E35-M60	1.9937	1.3928	0.2479	0.3944	158	99.37
E37-M49	1.9953	1.5302	0.3144	0.4765	213	99.53
E37-M59	2.0000	1.5961	0.3452	0.5156	246	100.00
E37-M60	1.9817	1.5571	0.3252	0.4890	214	98.17
E38-M48	1.9942	1.5507	0.3236	0.4881	172	99.42
E38-M49	1.9913	1.4182	0.2662	0.4199	229	99.13
E38-M60	1.9850	1.4554	0.2872	0.4473	263	98.50
E75-M55	1.9895	1.4117	0.2613	0.4130	283	98.95

AFLP Analysis of Genetic Resources of Dendrobium from China

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 20

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Rui, HONG Wenjuan, LUO Hua, ZHAO Lina, CHEN Ying, and WANG Jun, . Construction of SSR Fingerprints of Pomegranate Cultivars and Male Parent Identification of Hybrids [J]. Acta Horticulturae Sinica, 2023, 50(2): 265-278.
[2]	WANG Mengmeng, SUN Deling, CHEN Rui, YANG Yingxia, ZHANG Guan, LÜ Mingjie, WANG Qian, XIE Tianyu, NIU Guobao, SHAN Xiaozheng, TAN Jin, and YAO Xingwei, . Construction and Evaluation of Cauliflower Core Collection [J]. Acta Horticulturae Sinica, 2023, 50(2): 421-431.
[3]	LI Wenting, LI Cuixiao, LIN Xiaoqing, ZHENG Yongqin, ZHENG Zheng, DENG Xiaoling. Population Genetic Structure of Xanthomonas citri pv. citri in Guangdong Province Based on the STR Locus [J]. Acta Horticulturae Sinica, 2022, 49(6): 1233-1246.
[4]	YANG Yi, LI Tingyao, LI Guojing, CHEN Hancai, SHEN Zhuo, ZHOU Xuan, WU Zengxiang, WU Xinyi, ZHANG Yan. Development and Application of Insertion-Deletion（InDel）Markers in Asparagus Bean Based on Whole Genome Re-sequencing Data [J]. Acta Horticulturae Sinica, 2022, 49(4): 778-790.
[5]	NIE Xinghua, LI Yiran, TIAN Shoule, WANG Xuefeng, SU Shuchai, CAO Qingqin, XING Yu, QIN Ling. Construction of DNA Fingerprint Map and Analysis of Genetic Diversity for Chinese Chestnut Cultivars（Lines） [J]. Acta Horticulturae Sinica, 2022, 49(11): 2313-2324.
[6]	LI Shifan, SHAO Yuchun, QI Haonan, YANG Lan, LI Xiaowei, XU Bingliang, CHEN Yahan. Detection and Genetic Diversity Analysis of Apple Necrotic Mosaic Virus in Gansu Province [J]. Acta Horticulturae Sinica, 2022, 49(11): 2431-2438.
[7]	HOU Tianze, YI Shuangshuang, ZHANG Zhiqun, WANG Jian, LI Chonghui. Selection and Validation of Reference Genes for RT-qPCR in Phalaenopsis- type Dendrobium Hybrid [J]. Acta Horticulturae Sinica, 2022, 49(11): 2489-2501.
[8]	GONG Jianying, WANG Huaxin, WANG Xiaoyu, LONG Dingjian, TANG Qiuming, CHEN Baoling, SHU Lihua. A New Dendrobium Cultivar‘Lüying’ [J]. Acta Horticulturae Sinica, 2022, 49(11): 2525-2526.
[9]	CHEN Mingkun, CHEN Lu, SUN Weihong, MA Shanhu, LAN Siren, PENG Donghui, LIU Zhongjian, AI Ye. Genetic Diversity Analysis and Core Collection of Cymbidium ensifolium Germplasm Resources [J]. Acta Horticulturae Sinica, 2022, 49(1): 175-186.
[10]	GONG Jianying, WANG Huaxin, LONG Dingjian, CHEN Guoyong, WANG Xiaoyu, CHEN Yuqi, SHU Lihua, TANG Qiuming, and LIU Hua. A New Dendrobium Cultivar‘GFRI Ziyu’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2943-2944.
[11]	LI Yanhong, NIE Jun, ZHENG Jinrong, TAN Delong, ZHANG Changyuan, SHI Liangliang, XIE Yuming. Genetic Diversity Analysis and Multivariate Evaluation of Cherry Tomato by Phenotypic Traits in South China [J]. Acta Horticulturae Sinica, 2021, 48(9): 1717-1730.
[12]	ZHAO Yujing, LU Yin, FENG Daling, LUO Lei, WU Fang, YANG Rui, CHEN Yueqi, SUN Xiaoxue, WANG Yanhua, CHEN Xueping, SHEN Shuxing, LUO Shuangxia, ZHAO Jianjun. Association Analysis Between Background Selection InDel Markers and Important Agronomic Traits in Inbred Lines of Chinese Cabbage [J]. Acta Horticulturae Sinica, 2021, 48(7): 1282-1294.
[13]	ZHAO Qing, DU Zhenzhen, LI Xixiang, SONG Jiangping, ZHANG Xiaohui, YANG Wenlong, JIA Huixia, WANG Haiping. Genetic Diversity of Garlic Germplasm Resources Based on SSRseq Molecular Markers [J]. Acta Horticulturae Sinica, 2021, 48(7): 1397-1408.
[14]	JIANG Xibing, ZHANG Pingsheng, XU Yang, WU Conglian, ZHANG Dongbei, GONG Bangchu, WU Kaiyun, LAI Junsheng. Genetic Diversity of F₁ Hybrids of Chestnut Based on SSR Markers [J]. Acta Horticulturae Sinica, 2021, 48(5): 897-907.
[15]	MA Zhimin, DUAN Yu, XU Jianjian, BIN Yu, ZHOU Changyong, SONG Zhen. The Rapid Detection of Xanthomonas citri ssp. citri（Xcc）Based on Recombinase Polymerase Amplification（RPA）Assay [J]. Acta Horticulturae Sinica, 2021, 48(3): 590-599.