Enhanced Dehydration Tolerance in Lemon by Overexpression of CrNCED1（9-cis-epoxycarotenoid dioxygenase gene）from Citrus reshni

doi:10.16420/j.issn.0513-353x.2019-0375

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (3): 551-561.doi: 10.16420/j.issn.0513-353x.2019-0375

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Enhanced Dehydration Tolerance in Lemon by Overexpression of CrNCED1（9-cis-epoxycarotenoid dioxygenase gene）from Citrus reshni

DAI Wenshan1,2，WANG Min1,2，and LIU Jihong1,*

1Key Laboratory of Horticultural Plant Biology，College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China；2National Navel Orange Engineering Research Center，College of Navel Orange，Gannan Normal University，Ganzhou，Jiangxi 341000，China

Online:2020-03-25 Published:2020-03-25

Abstract

Abstract: To explore the function of CrNCED1，a key enzyme gene during abscisic acid biosynthesis in Cleopatra Mandarin（Citrus reshni），the expression characteristics of CrNCED1 was analyzed. Transgenic plants were obtained by overexpression of CrNCED1 in lemon for assaying dehydration resistance. The results showed that CrNCED1 showed the highest expression levels in leaves of Citrus reshni. Transcript levels of CrNCED1 were induced by dehydration，low temperature and ABA，but not by salt stress，among which ABA treatment strongly induced the expression of CrNCED1. The ABA content of transgenic lemon plants overexpressing CrNCED1 was remarkably higher than that of wild type. Upon exposure to dehydration treatment，the transgenic lines displayed less severe leaf wilting degree，concurrent with noticeably lower relative water loss，MDA and reactive oxygen species contents，and substantially smaller stomatal aperture in comparison with the wild type，indicating that overexpression of CrNCED1 conferred enhanced dehydration resistance in transgenic lemon plants.

Key words: Citrus reshni, lemon, CrNCED1, dehydration, abiotic stress, abscisic acid, functional analysis

CLC Number:

S 666

DAI Wenshan1,2，WANG Min1,2，and LIU Jihong1,*. Enhanced Dehydration Tolerance in Lemon by Overexpression of CrNCED1（9-cis-epoxycarotenoid dioxygenase gene）from Citrus reshni[J]. ACTA HORTICULTURAE SINICA, 2020, 47(3): 551-561.

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Enhanced Dehydration Tolerance in Lemon by Overexpression of CrNCED1（9-cis-epoxycarotenoid dioxygenase gene）from Citrus reshni

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