Abstract: Forty-seven accessions of rootstock-used pumpkin were collected in this research. Analyses
of genetic diversity and relationships were done with 63 phenotypes and 40 SSR markers. It was observed
that H’> 1.00 with eight qualitative characters（tender gourd skin color，fruit shape，main vine color，
skin speckle，foliage white spotting，leaf shape，tender gourd speckle and color）and 23 quantitative
characters. One hundred and sixty-seven polymorphic bands were generated. An average of 4.18
polymorphic bands were detected from one pair SSR primers，and they ranged from 2 to 8. H’ranged from
0.16 to 1.18，and its average was 0.62. Forty-seven germplasms were divided into four groups by 63
morphological traits，and most of the germplasms were clustered into one group. Three groups were
divided by 40 higher polymorphic SSR primers，similar to the result of our previous morphological study. The genetic diversity of collected rootstock-used pumpkin germplasms was low，and the genetic relationship
was close. SSR marker CMTm11，with a high polymorphism，could be used in molecular marker assisted
selection. Eight quality traits and most of the quantitative traits could be used as morphological markers，
as an auxiliary means of rootstock-used pumpkin germplasms evaluation and breeding of new varieties.

Key words: pumpkin, rootstock, phenotype, SSR